Differential retention of lymph-borne CD8 memory T cell subsets in the subcapsular sinus of resting and inflamed lymph nodes

2020 ◽  
Author(s):  
Ginka Nikolova ◽  
Siegfried Weiss ◽  
Berislav Bosnjak ◽  
Reinhold Förster
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
T Cell Subsets ◽  
Memory T Cell ◽  
Subcapsular Sinus ◽  
Differential Retention

scholarly journals CD8-positive memory T cells in tumor-draining lymph nodes of patients with breast cancer

2019 ◽  
Author(s):  
Yasmin Vahidi ◽  
Mandana Bagheri ◽  
Abbas Ghaderi ◽  
Zahra Faghih
Keyword(s):  
Breast Cancer ◽  
T Cells ◽  
T Cell ◽  
Lymph Nodes ◽  
Memory T Cells ◽  
T Cell Subsets ◽  
Axillary Lymph ◽  
Memory Cells ◽  
Memory T Cell ◽  
Draining Lymph Nodes

Abstract Background Human immunological memory is a hallmark of the adaptive immune system and plays an important role in the development of effective immune responses against tumors. In the present study, we aimed to determine the frequencies of CD8 + memory T cell subsets including stem memory T cells (TSCM) in tumor-draining lymph nodes of patients with breast cancer (BC).Methods Mononuclear cells were obtained from axillary lymph nodes of 52 untreated patients with BC and stained for CD8, CCR7, CD45RO, CD95 markers to detect different subtypes of memory cells in the CD8 + lymphocyte population. Data were acquired with four-color flow cytometry and analyzed with CellQuest Pro software.Results We observed that 47.65±2.66 of CD8+ lymphocytes expressed the CD45RO marker for memory T cells. Statistical analysis showed that the total frequency of central memory T cells (TCM) and their subset with low CD45RO expression was significantly higher in tumor-involved nodes compared to tumor-free ones (P=0.024 and P=0.017, respectively). Mean CD96 expression (based on mean fluorescence intensity) on the surface of TCM, their CD45RO hi TCM and CD45RO low subsets, and TSCM was higher in patients with stage II compared to those with stage I disease (P<0.05). The percentage of naive CD8 + T cells was significantly higher in tumor-involved lymph nodes compared to tumor-free ones (P=0.025).Conclusions Our data collectively indicate no significant differences in the frequencies of CD8 + lymphocytes or their memory T cell subsets in tumor-draining lymph nodes of patients with BC. However, the frequency of CD45 low TCM along with naive CD8 + lymphocytes was higher in tumor-involved nodes, which suggests that after long-term exposure to the antigen, and despite the immune reaction in order to provide a pool of effective memory cells, memory cell differentiation is blocked in early-stage (CD45RO low ) due to tumor-derived suppressive factors. Identifying the molecular and cellular mechanisms behind this suppression can provide invaluable tools for adoptive T cell therapies in cancer.

scholarly journals Altered levels of memory T cell subsets and common γc cytokines in Strongyloides stercoralis infection and partial reversal following anthelmintic treatment

2018 ◽  
Vol 12 (5) ◽  
pp. e0006481
Author(s):  
Anuradha Rajamanickam ◽  
Saravanan Munisankar ◽  
Yukti Bhootra ◽  
Chandra Kumar Dolla ◽  
Kannan Thiruvengadam ◽  
...  
Keyword(s):  
T Cell ◽  
Strongyloides Stercoralis ◽  
T Cell Subsets ◽  
Anthelmintic Treatment ◽  
Memory T Cell ◽  
Partial Reversal

Death of memory T-cell subsets in humans: changes during aging

2007 ◽  
Vol 3 (4) ◽  
pp. 637-645 ◽  
Author(s):  
Sudhir Gupta ◽  
Ankmalika A Gupta
Keyword(s):  
T Cell ◽  
T Cell Subsets ◽  
Memory T Cell

The Increase of Memory T Cell Subsets in Children with Idiopathic Nephrotic Syndrome

Nephron ◽  
1998 ◽  
Vol 79 (3) ◽  
pp. 274-278 ◽  
Author(s):  
Kunimasa Yan ◽  
Kazuhiko Nakahara ◽  
Shouichi Awa ◽  
Yukino Nishibori ◽  
Noriko Nakajima ◽  
...  
Keyword(s):  
Nephrotic Syndrome ◽  
T Cell ◽  
Idiopathic Nephrotic Syndrome ◽  
T Cell Subsets ◽  
Memory T Cell

T-cell subsets in lymph nodes identify a subgroup of follicular lymphoma patients with favorable outcome

2016 ◽  
Vol 58 (4) ◽  
pp. 842-850 ◽  
Author(s):  
Laura Magnano ◽  
Antonio Martínez ◽  
Joaquim Carreras ◽  
Alejandra Martínez-Trillos ◽  
Eva Giné ◽  
...  
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
Follicular Lymphoma ◽  
Favorable Outcome ◽  
T Cell Subsets

scholarly journals The Repertoires of Circulating Human CD8+ Central and Effector Memory T Cell Subsets Are Largely Distinct

Immunity ◽  
2003 ◽  
Vol 18 (2) ◽  
pp. 193-204 ◽  
Author(s):  
Véronique Baron ◽  
Cécile Bouneaud ◽  
Ana Cumano ◽  
Annick Lim ◽  
T.Petteri Arstila ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Subsets ◽  
Effector Memory ◽  
Memory T Cell

scholarly journals Comparison of selected CD45+ cell subsets’ response and cytokine levels on exhaustive effort among soccer players

2019 ◽  
Vol 38 (3) ◽  
pp. 256-267 ◽  
Author(s):  
Dorota Kostrzewa-Nowak ◽  
Rafał Buryta ◽  
Robert Nowak
Keyword(s):  
T Cell ◽  
Biological Response ◽  
T Cell Subsets ◽  
Alternative Mechanism ◽  
Post Exercise ◽  
Memory T Cell ◽  
Cell Percentage ◽  
Significant Release ◽  
Efficiency Test ◽  
The Impact

SummaryBackgroundImmunological alterations may led to the reduction in capacity and endurance levels in elite athletes by e.g. increased susceptibility to infections. There is a need to explain the impact of intensive physical effort on the CD4+memory T cell subsets.MethodsFourteen participants median aged 19 years old (range 17–21 years) were recruited form Pogoń Szczecin S.A., soccer club. They performed progressive efficiency test on mechanical treadmill until exhaustion twice: during preparatory phases to spring and autumn competition rounds. We examined the influence of exhaustive effort on the selected CD45+, especially CD4+memory T cell subsets and inflammation markers determined before, just after the test and during recovery time.ResultsSignificant changes in total CD45+cells and decrease in T lymphocytes percentage after the run was observed. Significant fluctuations in T cells’ distribution were related not only to the changes in Th or Tc subsets but also to increase in naïve T cell percentage during recovery. Increase in TNF-α and IL-8 post-exercise, IL-6 and IL-10 plasma levels in recovery was also found.ConclusionsThe novel finding of our study is that the run performed on mechanical treadmill caused a significant release of CD4+T naïve cells into circulation. Post-exercise increase in circulating NK cells is related with fast biological response to maximal effort. However, at the same time an alternative mechanism enhancing inflammation is involved.

scholarly journals Identification of Melanoma-reactive CD4+ T-Cell Subsets From Human Melanoma Draining Lymph Nodes

2016 ◽  
Vol 39 (1) ◽  
pp. 15-26 ◽  
Author(s):  
Mei Zhang ◽  
Hallie Graor ◽  
Lu Yan ◽  
Julian Kim
Keyword(s):  
T Cell ◽  
Lymph Nodes ◽  
Human Melanoma ◽  
T Cell Subsets ◽  
Cd4 T Cell ◽  
Draining Lymph Nodes

CD4+ T cells are essential for the development of destructive thyroiditis induced by anti–PD-1 antibody in thyroglobulin-immunized mice

2021 ◽  
Vol 13 (593) ◽  
pp. eabb7495
Author(s):  
Yoshinori Yasuda ◽  
Shintaro Iwama ◽  
Daisuke Sugiyama ◽  
Takayuki Okuji ◽  
Tomoko Kobayashi ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Lymph Nodes ◽  
Mononuclear Cells ◽  
Critical Role ◽  
T Cell Subsets ◽  
Effector Memory ◽  
Histopathological Analysis ◽  
Activated T Cells ◽  
Cervical Lymph Nodes

Immune-related adverse events induced by anti–programmed cell death–1 antibodies (PD-1-Ab), including destructive thyroiditis (thyroid-irAE), are thought to be caused by activated T cells. However, the T cell subsets that are directly responsible for damaging self-organs remain unclear. To clarify which T cell subsets are involved in the development of thyroid-irAE, a mouse model of thyroid-irAE was analyzed. PD-1-Ab administration 2.5 months after immunization with thyroglobulin caused destructive thyroiditis. Thyroiditis was completely prevented by previous depletion of CD4+ T cells and partially prevented by depleting CD8+ T cells. The frequencies of central and effector memory CD4+ T cell subsets and the secretion of interferon-γ after stimulation with thyroglobulin were increased in the cervical lymph nodes of mice with thyroid-irAE compared with controls. Histopathological analysis revealed infiltration of CD4+ T cells expressing granzyme B in thyroid glands and major histocompatibility complex class II expression on thyrocytes in mice with thyroid-irAE. Adoptive transfer of CD4+ T cells from cervical lymph nodes in mice with thyroid-irAE caused destruction of thyroid follicular architecture in the irradiated recipient mice. Flow cytometric analyses showed that the frequencies of central and effector memory CD4+ T cells expressing the cytotoxic marker CD27 were higher in peripheral blood mononuclear cells collected from patients with thyroid-irAE induced by PD-1-Ab versus those without. These data suggest a critical role for cytotoxic memory CD4+ T cells activated by PD-1-Ab in the pathogenesis of thyroid-irAE.

Sign in / Sign up

Export Citation Format

Share Document