EARLY BUD-BREAK 1 and EARLY BUD-BREAK 3 control resumption of poplar growth after winter dormancy

AbstractBud-break is an economically and environmentally important process in trees and shrubs from boreal and temperate latitudes, but its molecular mechanisms are poorly understood. Here, we show that two previously reported transcription factors, EARLY BUD BREAK 1 (EBB1) and SHORT VEGETATIVE PHASE-Like (SVL) directly interact to control bud-break. EBB1 is a positive regulator of bud-break, whereas SVL is a negative regulator of bud-break. EBB1 directly and negatively regulates SVL expression. We further report the identification and characterization of the EBB3 gene. EBB3 is a temperature-responsive, epigenetically-regulated, positive regulator of bud-break that provides a direct link to activation of the cell cycle during bud-break. EBB3 is an AP2/ERF transcription factor that positively and directly regulates CYCLIND3.1 gene. Our results reveal the architecture of a putative regulatory module that links temperature-mediated control of bud-break with activation of cell cycle.

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Abstract PR-008: Identification and characterization of the molecular mechanisms of SCLC chemo-radiation resistance

10.1158/1557-3265.radsci21-pr-008 ◽

2021 ◽

Author(s):

Francesca Anna Carrieri ◽

Nick Connis ◽

Eloise Grasset ◽

Eddie Luidy-Imada ◽

Andrew Ewald ◽

...

Keyword(s):

Radiation Resistance ◽

Molecular Mechanisms ◽

Identification And Characterization

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Identification and characterization of a negative regulator of FtsZ ring formation in Bacillus subtilis

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.96.17.9642 ◽

1999 ◽

Vol 96 (17) ◽

pp. 9642-9647 ◽

Cited By ~ 157

Author(s):

P. A. Levin ◽

I. G. Kurtser ◽

A. D. Grossman

Keyword(s):

Bacillus Subtilis ◽

Ring Formation ◽

Negative Regulator ◽

Ftsz Ring ◽

Identification And Characterization

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Identification and Characterization of a Paralog of Human Cell Cycle Checkpoint Gene HUS1

Genomics ◽

10.1006/geno.2002.6737 ◽

2002 ◽

Vol 79 (4) ◽

pp. 487-492 ◽

Cited By ~ 20

Author(s):

Haiying Hang ◽

Yuzhu Zhang ◽

Roland L. Dunbrack ◽

Cuidong Wang ◽

Howard B. Lieberman

Keyword(s):

Cell Cycle ◽

Human Cell ◽

Cell Cycle Checkpoint ◽

Checkpoint Gene ◽

Cycle Checkpoint ◽

Identification And Characterization

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Neuronal KGB-1 JNK MAPK signaling regulates the dauer developmental decision in response to environmental stress in C. elegans

10.1101/2021.06.28.450206 ◽

2021 ◽

Author(s):

Deepshikha Dogra ◽

Warakorn Kulalert ◽

Frank Schroeder ◽

Dennis H Kim

Keyword(s):

Elevated Temperature ◽

Signaling Pathways ◽

Mapk Pathway ◽

Negative Regulator ◽

Loss Of Function ◽

C Elegans ◽

Metabolic Remodeling ◽

Dauer Formation ◽

Identification And Characterization

In response to stressful growth conditions of high population density, food scarcity and elevated temperature, young larvae of nematode Caenorhabditis elegans can enter a developmentally arrested stage called dauer that is characterized by dramatic anatomic and metabolic remodeling. Genetic analysis of dauer formation of C. elegans has served as an experimental paradigm for the identification and characterization of conserved neuroendocrine signaling pathways. Here, we report the identification and characterization of a conserved JNK-like mitogen-activated protein kinase (MAPK) pathway that is required for dauer formation in response to environmental stressors. We observed that loss-of-function mutations in the MLK-1-MEK-1-KGB-1 MAPK pathway suppress dauer entry. Loss-of-function mutation in the VHP-1 MAPK phosphatase, a known negative regulator of KGB-1 signaling, results in constitutive dauer formation which is dependent on the presence of dauer pheromone but independent of diminished food levels or elevated temperatures. Our data suggest that KGB-1 pathway acts in the sensory neurons, in parallel to established insulin and TGF-β signaling pathways, to transduce the dauer-inducing environmental cues of diminished food levels and elevated temperature.

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Deciphering the pneumococcal cell cycle: Identification and characterization of new genes involved in growth and replication

10.33612/diss.127737312 ◽

2020 ◽

Author(s):

◽

Clément Gallay

Keyword(s):

Cell Cycle ◽

New Genes ◽

Identification And Characterization

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Identification and Characterization of Rice Circular RNAs Responding to Xanthomonas Oryzae pv. Oryzae Invasion

10.21203/rs.3.rs-164815/v1 ◽

2021 ◽

Author(s):

Peihong Wang ◽

Sai Wang ◽

Yan Wu ◽

Wenhan Nie ◽

Ayizekeranmu Yiming ◽

...

Keyword(s):

Molecular Mechanisms ◽

Expression Profiles ◽

Circular Rnas ◽

Pathogen Invasion ◽

Rice Leaves ◽

Transcriptional Gene Regulation ◽

Identification And Characterization ◽

Host Genes

Abstract BackgroundThe emerging role of circular RNAs (circRNAs) in various biological processes have advanced our knowledge of transcriptional and post-transcriptional gene regulation. The number and expression of plant circRNAs vary with species and treatments. However, the expression profile and the potential role of circRNAs during plant response to pathogen invasion are still elusive. ResultsIn this study, we identified 3517 circRNAs from PXO99A-infected rice leaves using the ribosomal RNA (rRNA) depleted RNA-Sequencing technique coupled with the CIRI2 and CIRCexplorer2 pipeline. Among them, 2994 (85.13%) circRNAs arised from the exons of their parent genes, 1214 circRNAs were previously unknown and 276 circRNAs exhibited differential expression profiles upon PXO99A infection over time. In addition, 31 differentially expressed circRNAs (DEcircRNAs) were predicted as the corresponding 121 miRNAs sponges. Functional analysis of both host genes and target mRNAs suggested that these identified circRNAs might play an important role in reprogramming rice responses to PXO99A invasion, mainly by mediating photorespiration, chloroplast, peroxisome and diterpenoid biosynthesis associated pathways.ConclusionThese results inferred a potential functional role of circRNAs in the regulation of rice immunity and provide novel clues for revealing the molecular mechanisms of rice-PXO99A interaction.

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Identification and characterization of microRNAs in white and black coated yak

Indian Journal of Animal Research ◽

10.18805/ijar.b-834 ◽

2018 ◽

Author(s):

Wang-Dui Basang ◽

Tian-Wu An ◽

Xiao-Lin Luo ◽

Yan-Bin Zhu ◽

Luo-Bu Danjiu Danjiu ◽

...

Keyword(s):

Molecular Mechanisms ◽

Coat Color ◽

Wnt Signaling Pathway ◽

Differentially Expressed ◽

Black And White ◽

Kegg Pathways ◽

Differentially Expressed Mirnas ◽

Paired End Sequencing ◽

Identification And Characterization

In this study, we used high-throughput technology to provide the first transcriptome dataset for differentially expressed miRNA in mixed pools of dermis tissue from black- and white-coated yak to research the possible molecular mechanisms of yak coat pigmentation. In this study, 92,636,002 and 95,917,842 clear reads were generated through Illumina paired-end sequencing. A total of 78 differentially expressed miRNAs (DEMs) were identified, including 59 upregulated and 19 downregulated miRNAs in the mixed pools of white-coated yak compared with the mixed pools of black-coated yak. In addition, 3634 genes were predicted as putative targets of DEMs. These DEGs related to 59 GO categories and were enriched in 216 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways, including melanogenesis and the Wnt signaling pathway. The results of the current study indicated that the coat color of the yak involved the transcriptional regulation process of miRNAs. These results provide helpful data to understand the molecular mechanisms of yak coat pigmentation.

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