scholarly journals Hyperproduction of 3-hydroxypropionate by Halomonas bluephagenesis

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xiao-Ran Jiang ◽  
Xu Yan ◽  
Lin-Ping Yu ◽  
Xin-Yi Liu ◽  
Guo-Qiang Chen
Keyword(s):  
Biosynthetic Pathway ◽  
Redox State ◽  
Continuous Process ◽  
Cell Mass ◽  
Degradation Pathway ◽  
Synthetic Enzymes ◽  
Platform Chemicals ◽  
Copolymer Poly ◽  
Hydroxypropionic Acid ◽  
Dry Cell

Abstract3-Hydroxypropionic acid (3HP), an important three carbon (C3) chemical, is designated as one of the top platform chemicals with an urgent need for improved industrial production. Halomonas bluephagenesis shows the potential as a chassis for competitive bioproduction of various chemicals due to its ability to grow under an open, unsterile and continuous process. Here, we report the strategy for producing 3HP and its copolymer poly(3-hydroxybutyrate-co-3-hydroxypropionate) (P3HB3HP) by the development of H. bluephagenesis. The transcriptome analysis reveals its 3HP degradation and synthesis pathways involving endogenous synthetic enzymes from 1,3-propanediol. Combing the optimized expression of aldehyde dehydrogenase (AldDHb), an engineered H. bluephagenesis strain of whose 3HP degradation pathway is deleted and that overexpresses alcohol dehydrogenases (AdhP) on its genome under a balanced redox state, is constructed with an enhanced 1.3-propanediol-dependent 3HP biosynthetic pathway to produce 154 g L−1 of 3HP with a yield and productivity of 0.93 g g−1 1,3-propanediol and 2.4 g L−1 h−1, respectively. Moreover, the strain could also accumulate 60% poly(3-hydroxybutyrate-co-32–45% 3-hydroxypropionate) in the dry cell mass, demonstrating to be a suitable chassis for hyperproduction of 3HP and P3HB3HP.

Quantitative Effects of Oxygen Supply on Mycelia Growth and Extracellular Polysaccharide of Ganoderma tsugae

2012 ◽  
Vol 217-219 ◽  
pp. 975-978
Author(s):  
Nukrob Narkprasom ◽  
Tzou Chi Huang ◽  
Yuan Kuang Guu
Keyword(s):  
Mass Transfer ◽  
Secondary Metabolite ◽  
Submerged Fermentation ◽  
Oxygen Supply ◽  
Cell Mass ◽  
Extracellular Polysaccharide ◽  
Mycelia Growth ◽  
High Level ◽  
Dry Cell

The quantitative effects of oxygen supply in terms of shaking speed and medium volume on the production of mycelia and extracellular polysaccharide (EPS) using Ganoderma tsugae in submerged fermentation were investigated. Mycelia growth required the proper shaking speed at 134 rpm for breaking a larger pellet into several smaller pellets. Furthermore, high level of medium volume for consumption of abundant nutrient is needed for maximum mycelia growth. For EPS, a high agitation is needed to promote a good mass transfer for achieving high product concentrations recovery. The appropriate medium volume was found to be 150.4 mL for aiding to produce a secondary metabolite by promoting the mass transfer of substrates. The dry cell mass (DCM)-EPS diagram of G. tsugae was created from the both production equations which this diagram is an useful tool for submerge fermentation industry for decision about to control the both productions.

Error in Measuring Dry Cell Mass with a Computerized Interference Microscope

2004 ◽  
Vol 47 (4) ◽  
pp. 412-416 ◽  
Author(s):  
G. G. Levin ◽  
A. A. Kovalev ◽  
V. L. Minaev ◽  
K. A. Sukhorukov
Keyword(s):  
Cell Mass ◽  
Interference Microscope ◽  
Dry Cell

Reactor-Scale Cultivation of the Hyperthermophilic MethanarchaeonMethanococcus jannaschii to High Cell Densities

1999 ◽  
Vol 65 (11) ◽  
pp. 5059-5065 ◽  
Author(s):  
Biswarup Mukhopadhyay ◽  
Eric F. Johnson ◽  
Ralph S. Wolfe
Keyword(s):  
Cell Mass ◽  
Ph Control ◽  
Growth Conditions ◽  
Cell Yield ◽  
Exhaust Gas ◽  
High Cell ◽  
Cell Extracts ◽  
Cell Densities ◽  
Acidic Compounds ◽  
Dry Cell

ABSTRACT For the hyperthermophilic and barophilic methanarchaeonMethanococcus jannaschii, we have developed a medium and protocols for reactor-scale cultivation that improved the final cell yield per liter from ∼0.5 to ∼7.5 g of packed wet cells (∼1.8 g dry cell mass) under autotrophic growth conditions and to ∼8.5 g of packed wet cells (∼2 g dry cell mass) with yeast extract (2 g liter−1) and tryptone (2 g liter−1) as medium supplements. For growth in a sealed bottle it was necessary to add Se to the medium, and a level of 2 μM for added Se gave the highest final cell yield. In a reactor M. jannaschii grew without added Se in the medium; it is plausible that the cells received Se as a contaminant from the reactor vessel and the H2S supply. But, for the optimal performance of a reactor culture, an addition of Se to a final concentration of 50 to 100 μM was needed. Also, cell growth in a reactor culture was inhibited at much higher Se concentrations. These observations and the data from previous work with methanogen cell extracts (B. C. McBride and R. S. Wolfe, Biochemistry 10:4312–4317, 1971) suggested that from a continuously sparged reactor culture Se was lost in the exhaust gas as volatile selenides, and this loss raised the apparent required level of and tolerance for Se. In spite of having a proteinaceous cell wall,M. jannaschii withstood an impeller tip speed of 235.5 cms−1, which was optimal for achieving high cell density and also was the higher limit for the tolerated shear rate. The organism secreted one or more acidic compounds, which lowered pH in cultures without pH control; this secretion continued even after cessation of growth.

PREPARATION AND EVALUATION OF A MICROBIOLOGICAL GROWTH MEDIUM FORMULATED FROM CATFISH WASTE PEPTONE

1974 ◽  
Vol 37 (5) ◽  
pp. 277-281 ◽  
Author(s):  
L. R. Beuchat
Keyword(s):  
Ictalurus Punctatus ◽  
Channel Catfish ◽  
Cell Mass ◽  
Water Soluble ◽  
Heat Stable ◽  
Luxuriant Growth ◽  
Test Organisms ◽  
Preparation And Evaluation ◽  
Dry Cell ◽  
Proximate Analyses

A water-soluble, heat-stable peptone was extracted-from enzymatically digested channel catfish (Ictalurus punctatus) heads and skins. Proximate analyses showed that the peptone was composed of 89.02% protein (Kjeldahl N × 6.25), 10.42% ash and 0.44% fat. The peptone contains all amino acids demonstrated to be present in concentrations of greater than 1.0% in six other commercially available microbiological peptones used for comparative purposes in the study. Total cell mass production on catfish and test peptone media by microorganisms representing 13 genera was measured. Catfish peptone supported luxuriant growth of the test organisms and an accumulative average of dry cell mass weights produced by the organisms revealed that the catfish peptone ranked third out of the seven peptones tested. This new peptone offers the clinical and industrial microbiologist a potentially valuable ingredient for formulation of growth and fermentation media.

Biotransformation of iminodiacetonitrile to iminodiacetic acid by Alcaligenes faecalis cells immobilized in ACA-membrane liquid-core capsules

2014 ◽  
Vol 68 (1) ◽  
Author(s):  
Jin-Feng Zhang ◽  
Zhi-Qiang Liu ◽  
Xin-Hong Zhang ◽  
Yu-Guo Zheng
Keyword(s):  
Cell Mass ◽  
Liquid Core ◽  
Iminodiacetic Acid ◽  
Alcaligenes Faecalis ◽  
Packed Bed Reactor ◽  
Whole Cells ◽  
Encapsulated Cells ◽  
Free Cells ◽  
Nitrilase Activity ◽  
Dry Cell

AbstractBiotransformation of iminodiacetonitrile (IDAN) to iminodiacetic acid (IDA) was investigated with a newly isolated Alcaligenes faecalis ZJUTBX11 strain showing nitrilase activity in the immobilized form. To reduce the mass transfer resistance and to increase the toleration ability of the microorganisms to the toxic substrate as well as to enhance their ability to be reused, encapsulation of the whole cells in alginate-chitosan-alginate (ACA) membrane liquid-core capsules was attempted in the present study. The optimal pH and temperature for nitrilase activity of encapsulated A. faecalis ZJUTBX11 cells were 7.5°C and 35°C, respectively, which is consistent with free cells. Based on the Michaelis-Menten model, kinetic parameters of the conversion reaction with IDAN as the substrate were: K m = (17.6 ± 0.3) mmol L−1 and V max = (97.6 ± 1.2) μmol min−1 g−1 of dry cell mass for encapsulated cells and (16.8 ± 0.4) mmol L−1 and (108.0 ± 2.7) μmol min−1 g−1 of dry cell mass for free cells, respectively. After being recycled ten times, the whole cells encapsulated in ACA capsules still retained 90 % of the initial nitrilase activity while only 35 % were retained by free cells. Lab scale production of IDA using encapsulated cells in a bubble column reactor and a packed bed reactor were performed respectively.

scholarly journals Isolation and characterization of Acetobacter aceti from rotten papaya

2016 ◽  
Vol 13 (2) ◽  
pp. 299-306 ◽  
Author(s):  
J Kowser ◽  
MG Aziz ◽  
MB Uddin
Keyword(s):  
Cell Mass ◽  
Single Pair ◽  
Acetobacter Aceti ◽  
Biochemical Tests ◽  
Isolation And Characterization ◽  
The Media ◽  
Durham Tube ◽  
Successive Subculture ◽  
Dry Cell

The present study was concerned with the isolation and characterization of Acetobacter aceti from rotten papaya for vinegar production. The samples were inoculated in sterilized GYC standard media and then incubated at 30°C for 48 hours. Successive subculture was performed to screen out the strains. In Gram’s staining, the morphology of the isolated bacteria exhibited pink, small rod shaped single, pair and chain in arrangement, in the hanging drops technique, all the isolates revealed motile. Biochemical tests were performed by fermentation of five basic sugars by producing both acid and gas bubbles in Durham tube. All of the isolates were Indole, Voges-Proskauer (VP) and Oxidase negative, Methyl Red (MR) and Catalase positive. The growth rate of isolated strain was optimized by weighing dry cell and turbidity at 600 nm at different concentrations of dextrose (1%, 5% and 10%). Ten (10) percent dextrose solution showed rapid growth and higher cell mass than 5% and 1% solution respectively. Acidity of the media gradually increased from 0.102% to 2.18% from day 0 to day 7 and pH of the media decreased from 6.8 to 5.5 during the period. This protocol was successful for enriching Acetobacter aceti, which was essential for vinegar production.J. Bangladesh Agril. Univ. 13(2): 299-306, December 2015

Fermentation of natural gas with a cyclone column fermenter

1969 ◽  
Vol 15 (9) ◽  
pp. 1047-1050 ◽  
Author(s):  
J. C. Mueller
Keyword(s):  
Sewage Sludge ◽  
Natural Gas ◽  
Doubling Time ◽  
Cell Mass ◽  
Interfacial Area ◽  
Carbon Substrate ◽  
Cell Concentrations ◽  
Column Fermenter ◽  
Dry Cell

A mixed flora, capable of using natural gas as a carbon substrate, was derived from a laboratory sewage sludge. Fermentations in a cyclone column fermenter, on a 1:1 natural gas/air mixture, resulted in a doubling time of 4.6 h and production of 0.065 mg dry cell mass per square centimeter interfacial area per hour. Foaming problems limited cell concentrations to 6.1 g/l.

scholarly journals Lipid Accumulation Bioprocess of Oils and Fats: From the State of the Art to the Challenges

2020 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Lipid Accumulation ◽  
State Of The Art ◽  
Cell Mass ◽  
Raw Material ◽  
Chemical Production ◽  
Oleaginous Microorganisms ◽  
Dry Cell Weight ◽  
Potential Applications ◽  
Alternative Sources ◽  
Dry Cell

Microorganisms, including yeasts and bacteria, have long been studied as alternative sources of oils and fats [1, 2]. Microorganisms synthesize lipids as a part of their metabolism, and as a source of energy. Some species have been reported to accumulate more than 20% of their dry cell mass in the form of lipids, and have been classified as “oleaginous” microorganisms [3]. Moreover, some oleaginous yeast species are particularly promising in this respect, as they can accumulate more than 70% of their dry cell weight as lipids [3]. In addition to this considerable capacity for lipid accumulation, oleaginous yeasts present various fatty acid profiles. In particular, they synthesize valuable polyunsaturated fatty acids, and are, therefore, a target of choice for potential applications as a renewable raw material for energetic and chemical production or as nutritional supplements. The analysis of the international state of the art revealed that oleaginous microorganisms have been studied over decades.

scholarly journals Production of Biodegradable Polymer From Agro-Wastes in Alcaligenes sp. and Pseudomonas sp.

2021 ◽  
Author(s):  
R.Z. Sayyed ◽  
S. S. Shaikh ◽  
S. J. Wani ◽  
Md Tabish Rehman ◽  
Mohamed F. Alajmi ◽  
...  
Keyword(s):  
Rice Straw ◽  
Vegetable Oils ◽  
Large Scale ◽  
Low Cost ◽  
Mass Medium ◽  
Cell Mass ◽  
Cost Effective ◽  
Alcaligenes Faecalis ◽  
Pseudomonas Sp ◽  
Dry Cell

The present study was aimed to evaluate the suitability of agro-wastes and crude vegetable oils for the cost effective production of poly-β-hydroxybutyrate (PHB), to evaluate growth kinetics and PHB production in Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 with these carbon substrates and to study the biodegradation of PHB accumulated by these cultures. Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1 accumulate higher amounts of PHB corn (79.90% of dry cell mass) and rice straw (66.22% of dry cell mass) medium respectively. The kinetic model suggests that the Pseudomonas sp. RZS1 follows the Monod model more closely than A. faecalis RZS4. Both the cultures degrade their own PHB extract under the influence of PHB depolymerase. Corn waste and rice straw appear as the best and cost-effective substrates for the sustainable production of PHB from Alcaligenes faecalis RZS4 and Pseudomonas sp. RZS1. The biopolymer accumulated by these organisms is biodegradable in nature. The agro-wastes and crude vegetable oils are good and low cost sources of nutrients for the growth and production of PHN and other metabolites. Their use would lower the production cost of PHN and the low cost production will reduce the sailing price of PHB based products. This would promote the large scale commercialization and popularization of PHB as ecofriendly bioplastic/biopolymer.

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