Evaluation of methanolic crude extract of Linum usitatissimum for the removal of biofilm in diabetic foot isolates

Linum usitatissimum L is a widely used traditionally for multiple ailments. The present research was carried out to explore the antimicrobial, and anti-biofilm activity of crude extract of Linum usitatissimum L (Lu. Cr). Phytochemical and proximate analyses were performed. The bandages of diabetic foot patients were collected from the various hospitals. The bandages were cultured to isolate the bacterial strains present on it. The disc diffusion method was used to identify the antimicrobial potential whereas the minimum inhibitory concentration of the Lu.Cr were also determined. Proximate analysis confirms moisture content 8.33%, ash content 4.33%, crude protein 21.20%, crude fat 49.2% and crude fiber 5.63%. It was revealed that Gram-positive bacteria are most prevalent among all study groups. Lu.Cr possess significant bactericidal potential against S. aureus among all other microbes. Owing to this potential, linseed coated bandages can be used alternatively for the treatment of diabetic foot.

Studies on the Nutritional, Mineral Composition, Mineral Ratio and Anti-nutritional Molar Ratio of Six Underutilized Wild Edible Vegetables in Ado-Ekiti, Ekiti State, Nigeria

Aim: Fruits and common vegetables are now becoming too expensive for the poor consumers in Nigeria. The search for cheap, readily available and (locally sourced for the people in need of) nutritious food prompted this research work. Study Design: Six underutilized wild vegetables (Solanum microcarpon, Strichium sparganophora, Crassocephalum crepidiodes, Solanum nigrum, Myrianthus arboreus and Sterculia tragacantha) from Ado- Ekiti, Ekiti State, Nigeria were evaluated for their nutritional, mineral and anti-nutritional potentials. Place and Duration of Study: The collection of the samples were done in the month of December, 2020 in Ado Ekiti, Ekiti State, Nigeria and air dried for three weeks. Methodology: Young shoots of five indigenous vegetables were collected, washed thoroughly, air dried, pulverized and analysed using standard methods of AOAC. Results: The proximate analyses of the vegetables indicated that the moisture content, ash, fat, crude fiber, crude protein and carbohydrates ranged as follows: 6.17-8.85%, 4.73-6.59 %, 2.00-3.13%, 3.89-7.27%, 7.77-12.23% and 63.84-70.61% respectively. The total energy ranged from 1,367.85 to 1,467.85KJ/100g with low Coefficient of Variation (CV%) of 2.22. Mineral contents are of the following order K>Mg>Ca> P>Na>Fe>Zn. The result of the mineral ratio showed that the calculated mineral ratio for Na/K, K/ Na, Ca/P, Ca/Mg, Ca/K, Zn/Cu and [K/(Ca+Mg)] were below the critical level. The calculated mineral safety indexes (MSI) were lower than the tabulated values. The anti-nutrient contents (Cyanide, Saponins, and Oxalate) were below the threshold levels, Conclusion: Low MSI means that the vegetables could not pose health risk when consumed. The low anti-nutrient contents of the samples is an indication that the consumer would not suffer from mineral overload. The nutrition and mineral potentials of these vegetables could suggest their uses as nutritional supplements and are highly promising for food security and sustainability for the populace.

Torrefaction of Oil Palm Frond Petiole: Effect of Particle Sizes, Sections and Batches

This study aimed to investigate the effect of batches (1, 2 and 3), particle sizes (<250 µm, range of 300 µm to 500 µm), and sections (bottom, middle and top) on combustion performance of the oil palm frond (OPF) petiole after torrefaction at 275 °C. The higher heating value (HHV), mass yield, energy yield, HHV yield and proximate analyses of the untorrefied and torrefied OPF petiole for all cases were determined. The comparison between the untorrefied and torrefied OPF petiole as well as an international benchmark was also performed. In this study, the highest HHV of the torrefied OPF petiole (22.85±0.07MJ/kg) was obtained at the bottom section with the particle size of < 250 µm. Furthermore, the fixed carbon content of the torrefied OPF petiole increased, whereas the volatile matter, moisture content, mass and energy yields decreased for all cases after torrefaction. HHV yield of OPF petiole was recorded up to 141% after torrefaction. The ash content was sufficiently satisfied the international benchmark for most cases, except for top section (300-500µm). The changes in combustion properties of the torrefied OPF petiole for all cases were found to be insignificant whereas significant improvement could be observed when compared to untorrefied OPF petiole. Overall, the study revealed that the appropriate particle size for torrefaction can promote it to be a vital source for energy production from oil palm biomass.

EFFECT OF THE DRYING METHOD ON THE QUALITY AND DRYING CHARACTERISTIC OF MINT LEAVES (Mentha spicata L.)

Mint leaves are vegetables used for tea especially in the northern part of Nigerian because of their nutritional and medicinal values. Fresh mint leaves are perishable and to extend their shelf-life drying the leaves is a common practice among the people. The application of a proper drying method is essential to preserve its nutritional and medicinal values. Lack of information on the drying characteristic that could be used for the design of its dryers is a major bottleneck in its processing. This accounts for the poor colour, taste, flavour of dried mint leaves found in the market.In this study, three methods were used to dry mint leaves, sun, oven and shade drying methods. Proximate analyses were carried out on the samples dried with the three methods. Samples drying characteristics were investigated using an oven at 40, 50, 60 and 70 °C. The results show that the drying method significantly affected all the proximate compositions at 5% level. Shade drying has the highest crude protein and ash contents of 7.74% and 8.48% respectively. Carbohydrates were more (30.13%) when open sun drying was employed. Oven drying favoured lowest moisture content of 7.20%, highest crude fiber of 49.34% and highest crude fat contents of 9.22%. To dry mint leaves to equilibrium moisture content, it took 140 min for samples at a drying temperature of 40 °C, 120 min at 50 °C, 90 min at 60 °C and 70 min for 70 °C. Drying of the leaves occurred in a falling rate period.

Performance of Some Biocoal Briquettes Produced from Mixture of Sawdust and varying Nigeria Coals as Composite Domestic Fuel

Performance evaluation of biocoal briquettes from three different coal mines in Nigeria mixed with sawdust as composite domestic fuel was carried out. Samples of coals collected from Owukpa, Okpara, and Onyeama mines were pulverized and blended with sawdust at various constituent ratios of coal/sawdust of 0.0/100, 10/90, 20/80, 30/70, 40/60, 50/50 and 100/0.0. Cassava starch was used as binding material while calcium hydroxide was used for desulphurization. Tests carried out included proximate analyses, ultimate analyses, and performance analyses. Results showed that fixed carbon in all the coal samples ranges from the highest fixed carbon of 63.81% for Okpara (OK) to the lowest of 62.87% for Owukpa (OP70/30) while sawdust was 21.03%. The Onyeama Coal has 1.25% ash content compares with 5% for Owukpa. Onyeama coal contains the highest volatile matters of 29.61% while Owukpa biocoal briquette (OP50/50) has the highest percentage heat utilization of 88.61 and lowest specific fuel consumption of 0.31kg/kg. Onyeama coal (ON90/10) has the highest calorific value of 33.25 MJ/kg. The addition of sawdust in the biocoal briquettes resulted in lower volatile matter, low hydrogen, high percentage heat utilized and low specific fuel consumption.Keywords- Biocoal briquette, sawdust, proximate analysis, ultimate analysis, fixed carbon

Compost of Biodegradable Municipal Solid Waste as a Fuel in Lignite Co-combustion

In Greece, 5.8 million tons of municipal solid wastes (MSW) are produced annually, of which 2.47 million tons are bio-wastes. Composting is an alternative treatment of bio-wastes instead of landfill. Τwo composting plants operate in Greece, one in Ano Liossia (capacity 1,200 tons/day, producing 120 tpd compost) and another in Chania (capacity 70,000 tons/year, producing 20,000 tpa compost). In addition, since 2018, the first integrated waste management plant was set off in the region of Kozani (capacity 120,000 tons/year). An alternative utilization of the compost, produced in the latter plant, was investigated in this study. In particular, instead of using compost as fertilizer, the energy recovery from this bio-waste was attested. Utilization of compost of MSW for energy production purposes has rarely been studied in the literature. Several blends of compost with lignite were prepared and their energetic potential was determined. Proximate analyses and gross calorific value (GCV) determination were conducted. Wastes and biomass-based fuels differ in many ways from fossil fuels. The CLOF sample revealed the highest GCV and the lowest ash content than all analyzed samples. Based on all analytical determinations, compost and its mixtures with lignite could be regarded suitable for energy recovery by thermal processes, such us combustion. Further studies should be done including emission analysis, ash deposition during combustion (corrosion, slagging and fouling).

Proximate, Mineral Composition and Phytochemical Screening of Aqueous Leaf Extract of Alafia barteri Oliv. (Apocynaceae)

Alafia barteri leaves are valued for its efficacy in the traditional medicine system in Nigeria. It has been used over the years for the treatment of various diseases some of which include malaria, sickle cell anaemia, and eye infections. This has necessitated the evaluation of its chemical composition to ensure its safety for human use. This study was undertaken to evaluate the phytochemical constituents, proximate and elemental composition of Alafia barteri leaf. The phytochemicals and proximate analyses were carried out using standard methods. The mineral elements were analyzed using atomic absorption spectrophotometry. The proximate analysis of the leaves showed it contained moisture 8.6%, crude fibre 48.29%, protein 15.84%, carbohydrate 13.13%, lipids 7.23% and ash 6.75%. The mineral analysis of the leaves revealed the presence of the following minerals: calcium (96.2 mg/100 g), manganese (74.8 mg/100 g), iron (46.2 mg/100 g), magnesium (31.05 mg/100 g), potassium (3.3 mg/ 100 g), sodium (2.9 mg/100 g). The phytochemical screening indicated the presence of flavonoids, phenols, tannins, anthraquinones, phlobatannins, cardiac glycosides and steroids. The study revealed the leaves of Alafia barteri to be a potential source of minerals and nutrients needed for human nutrition.