scholarly journals Lipid Accumulation Bioprocess of Oils and Fats: From the State of the Art to the Challenges

2020 ◽  
Vol 3 (1) ◽  
Keyword(s):  
Lipid Accumulation ◽  
State Of The Art ◽  
Cell Mass ◽  
Raw Material ◽  
Chemical Production ◽  
Oleaginous Microorganisms ◽  
Dry Cell Weight ◽  
Potential Applications ◽  
Alternative Sources ◽  
Dry Cell

Microorganisms, including yeasts and bacteria, have long been studied as alternative sources of oils and fats [1, 2]. Microorganisms synthesize lipids as a part of their metabolism, and as a source of energy. Some species have been reported to accumulate more than 20% of their dry cell mass in the form of lipids, and have been classified as “oleaginous” microorganisms [3]. Moreover, some oleaginous yeast species are particularly promising in this respect, as they can accumulate more than 70% of their dry cell weight as lipids [3]. In addition to this considerable capacity for lipid accumulation, oleaginous yeasts present various fatty acid profiles. In particular, they synthesize valuable polyunsaturated fatty acids, and are, therefore, a target of choice for potential applications as a renewable raw material for energetic and chemical production or as nutritional supplements. The analysis of the international state of the art revealed that oleaginous microorganisms have been studied over decades.

scholarly journals Fast media optimization for mixotrophic cultivation of Chlorella vulgaris

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Valerie C. A. Ward ◽  
Lars Rehmann
Keyword(s):  
Cell Density ◽  
Lipid Content ◽  
Lipid Accumulation ◽  
Accumulation Rate ◽  
Lipid Production ◽  
Total Lipid Content ◽  
Mixotrophic Cultivation ◽  
Cell Weight ◽  
Dry Cell Weight ◽  
Dry Cell

AbstractMicroalgae can accumulate large proportions of their dry cell weight as storage lipids when grown under appropriate nutrient limiting conditions. While a high ratio of carbon to nitrogen is often cited as the primary mode of triggering lipid accumulation in microalgae, fast optimization strategies to increase lipid production for mixotrophic cultivation have been difficult to developed due to the low cell densities of algal cultures, and consequently the limited amount of biomass available for compositional analysis. Response surface methodologies provide a power tool for assessing complex relationships such as the interaction between the carbon source and nitrogen source. A 15 run Box-Behnken design performed in shaker flasks was effective in studying the effect of carbon, nitrogen, and magnesium on the growth rate, maximum cell density, lipid accumulation rate, and glucose consumption rate. Using end-point dry cell weight and total lipid content as assessed by direct transesterification to FAME, numerical optimization resulted in a significant increase in lipid content from 18.5 ± 0.76% to 37.6 ± 0.12% and a cell density of 5.3 ± 0.1 g/L to 6.1 ± 0.1 g/L between the centre point of the design and the optimized culture conditions. The presented optimization process required less than 2 weeks to complete, was simple, and resulted in an overall lipid productivity of 383 mg/L·d.

scholarly journals Purification of recombinant human fibroblast growth factor-2 from one-liter fermentation broth

2015 ◽  
Vol 18 (1) ◽  
pp. 14-22 ◽  
Author(s):  
Thy Tien Anh Tran ◽  
Trinh Thi My Nguyen ◽  
Hieu Van Tran
Keyword(s):  
Growth Factor ◽  
Fibroblast Growth Factor ◽  
Fermentation Broth ◽  
Cell Mass ◽  
Coli Strain ◽  
Fibroblast Growth Factor 2 ◽  
Fibroblast Growth ◽  
Multifunctional Protein ◽  
Dry Cell Weight ◽  
Dry Cell

Fibroblast Growth Factor - 2 (FGF-2), also known as basic FGF is a multifunctional protein that regulates the proliferation and differentiation of multiple types of cell. Recombinant human FGF-2 (rhFGF-2) is currently used in medicine, cosmetics, stem-cell culture, etc. In this study, we conducted one-liter scale fermentation using Escherichia coli strain that carries recombinant vector harboring FGF-2 coding gene to produce a large amount of FGF-2 protein. The evaluation of the fermentation efficacy was based on the growth curve, dry cell weight and amount of FGF-2 obtaining from one-liter fermentation. After fermentation, cell mass was lyzed by high pressure. Then, the FGF-2 in supernatant was purified by cation exchange and heparin-affinity chromatography. The purity and efficiency of the purification process were estimated by Bradford, silver staining and densitometry using QuantityOne software. The result showed that in oneliter fermentation, we obtained 5.2 g/liter dry cell-mass and 230 mg/liter FGF-2. The purity of FGF-2 was about 97.1 % and the purification efficiency was above 46.08 %.

RHEOLOGICAL PROPERTIES OF CERAMIC MASSES: IMPROVEMENT BY COMPLEX MATERIAL ACTIVATION

2019 ◽  
Vol 16 (3) ◽  
pp. 334-351
Author(s):  
A. S. Mavlyanov ◽  
E. K. Sardarbekova
Keyword(s):  
Rheological Properties ◽  
Raw Materials ◽  
Sensitivity Coefficient ◽  
Raw Material ◽  
Clay Material ◽  
Fibrous Materials ◽  
Chemical Production ◽  
Production Wastes ◽  
Pass Through ◽  
The Masses

Introduction. The objective of the research is to study the effect of the complex activation of the alumina raw material on the rheological properties of the ceramic mass. In addition, the authors investigate solutions for the application of optimal coagulation structures based on loams and ash together with plastic certificates.Materials and methods. The authors used the local forest like reserves of clay loams at the BashKarasu, ash fields of the Bishkek Central Heating Centre (BTEC) and plasticizer (sodium naphthenate obtained from alkaline chemical production wastes) as fibrous materials. Moreover, the authors defined technological properties of raw materials within standard laboratory methodology in accordance with current GOSTs.Results. The researchers tested plastic durability on variously prepared masses for the choice of optimal structures. The paper demonstrated the plastic durability of complexly activated compounds comparing with non-activated and mechanically activated compounds. The sensitivity coefficient increased the amount of clay loams by mechanically and complexly activated, which predetermined the possibility of intensifying the process of drying samples based on complexly activated masses.Discussion and conclusions. However, mechanical activation of clay material reduces the period of relaxation and increases the elasticity coefficient of ceramic masses by 1.8–3.4 times, meanwhile decreases elasticity, viscosity and the conventional power during molding, which generally worsens the molding properties of the masses. Сomplex activation of ash-clay material decreases the period of relaxation and provides an increase in elasticity, plasticity of ceramic masses by 46–47%, reduction in viscosity by 1.5–2 times, conventional power on molding by 37–122% in comparison with MA clay loams. Ceramic masses based on spacecraft alumina raw materials belong to the SMT with improved rheological properties; products based on them pass through the mouthpiece for 5–7 seconds.

The interasin raw material base of coke-chemical production of Ukraine: Problems of charge formation, its preparation and coking

2018 ◽  
Vol 5 (6) ◽  
pp. 3-7 ◽  
Author(s):  
E.S. Popov ◽  
V.I. Gavrilyuk ◽  
N.V. Mukina ◽  
E.T. Kovalev ◽  
I.D. Drozdnik ◽  
...  
Keyword(s):  
Raw Material ◽  
Material Base ◽  
Chemical Production ◽  
Charge Formation

scholarly journals In vivo interactome profiling by enzyme‐catalyzed proximity labeling

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yangfan Xu ◽  
Xianqun Fan ◽  
Yang Hu
Keyword(s):  
State Of The Art ◽  
Catalytic Efficiency ◽  
Biological Processes ◽  
Protein Protein Interaction ◽  
Current State ◽  
Protein Interactome ◽  
Potential Applications ◽  
Protein Protein Interaction Networks ◽  
Temporal And Spatial

AbstractEnzyme-catalyzed proximity labeling (PL) combined with mass spectrometry (MS) has emerged as a revolutionary approach to reveal the protein-protein interaction networks, dissect complex biological processes, and characterize the subcellular proteome in a more physiological setting than before. The enzymatic tags are being upgraded to improve temporal and spatial resolution and obtain faster catalytic dynamics and higher catalytic efficiency. In vivo application of PL integrated with other state of the art techniques has recently been adapted in live animals and plants, allowing questions to be addressed that were previously inaccessible. It is timely to summarize the current state of PL-dependent interactome studies and their potential applications. We will focus on in vivo uses of newer versions of PL and highlight critical considerations for successful in vivo PL experiments that will provide novel insights into the protein interactome in the context of human diseases.

scholarly journals Efficient Graph Collaborative Filtering via Contrastive Learning

Sensors ◽  
2021 ◽  
Vol 21 (14) ◽  
pp. 4666
Author(s):  
Zhiqiang Pan ◽  
Honghui Chen
Keyword(s):  
Collaborative Filtering ◽  
State Of The Art ◽  
Representation Learning ◽  
First Order ◽  
Satisfactory Performance ◽  
Potential Applications ◽  
Benchmark Datasets ◽  
Bayesian Personalized Ranking ◽  
Graph Neural Networks ◽  
Training Efficiency

Collaborative filtering (CF) aims to make recommendations for users by detecting user’s preference from the historical user–item interactions. Existing graph neural networks (GNN) based methods achieve satisfactory performance by exploiting the high-order connectivity between users and items, however they suffer from the poor training efficiency problem and easily introduce bias for information propagation. Moreover, the widely applied Bayesian personalized ranking (BPR) loss is insufficient to provide supervision signals for training due to the extremely sparse observed interactions. To deal with the above issues, we propose the Efficient Graph Collaborative Filtering (EGCF) method. Specifically, EGCF adopts merely one-layer graph convolution to model the collaborative signal for users and items from the first-order neighbors in the user–item interactions. Moreover, we introduce contrastive learning to enhance the representation learning of users and items by deriving the self-supervisions, which is jointly trained with the supervised learning. Extensive experiments are conducted on two benchmark datasets, i.e., Yelp2018 and Amazon-book, and the experimental results demonstrate that EGCF can achieve the state-of-the-art performance in terms of Recall and normalized discounted cumulative gain (NDCG), especially on ranking the target items at right positions. In addition, EGCF shows obvious advantages in the training efficiency compared with the competitive baselines, making it practicable for potential applications.

scholarly journals Separation and Efficient Recovery of Lithium from Spent Lithium-Ion Batteries

Metals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1091
Author(s):  
Eva Gerold ◽  
Stefan Luidold ◽  
Helmut Antrekowitsch
Keyword(s):  
Lithium Ion Batteries ◽  
Electric Vehicles ◽  
Sodium Phosphate ◽  
Room Temperature ◽  
State Of The Art ◽  
Potassium Phosphate ◽  
Lithium Ion ◽  
Rechargeable Batteries ◽  
Raw Material ◽  
Efficient Recovery

The consumption of lithium has increased dramatically in recent years. This can be primarily attributed to its use in lithium-ion batteries for the operation of hybrid and electric vehicles. Due to its specific properties, lithium will also continue to be an indispensable key component for rechargeable batteries in the next decades. An average lithium-ion battery contains 5–7% of lithium. These values indicate that used rechargeable batteries are a high-quality raw material for lithium recovery. Currently, the feasibility and reasonability of the hydrometallurgical recycling of lithium from spent lithium-ion batteries is still a field of research. This work is intended to compare the classic method of the precipitation of lithium from synthetic and real pregnant leaching liquors gained from spent lithium-ion batteries with sodium carbonate (state of the art) with alternative precipitation agents such as sodium phosphate and potassium phosphate. Furthermore, the correlation of the obtained product to the used type of phosphate is comprised. In addition, the influence of the process temperature (room temperature to boiling point), as well as the stoichiometric factor of the precipitant, is investigated in order to finally enable a statement about an efficient process, its parameter and the main dependencies.

scholarly journals Complete biosynthesis of a sulfated chondroitin in Escherichia coli

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Abinaya Badri ◽  
Asher Williams ◽  
Adeola Awofiranye ◽  
Payel Datta ◽  
Ke Xia ◽  
...  
Keyword(s):  
Chondroitin Sulfate ◽  
Scale Up ◽  
Production Methods ◽  
E Coli ◽  
Microbial Cell Factories ◽  
Cell Factories ◽  
Microbial Product ◽  
Dry Cell Weight ◽  
One Step ◽  
Dry Cell

AbstractSulfated glycosaminoglycans (GAGs) are a class of important biologics that are currently manufactured by extraction from animal tissues. Although such methods are unsustainable and prone to contamination, animal-free production methods have not emerged as competitive alternatives due to complexities in scale-up, requirement for multiple stages and cost of co-factors and purification. Here, we demonstrate the development of single microbial cell factories capable of complete, one-step biosynthesis of chondroitin sulfate (CS), a type of GAG. We engineer E. coli to produce all three required components for CS production–chondroitin, sulfate donor and sulfotransferase. In this way, we achieve intracellular CS production of ~27 μg/g dry-cell-weight with about 96% of the disaccharides sulfated. We further explore four different factors that can affect the sulfation levels of this microbial product. Overall, this is a demonstration of simple, one-step microbial production of a sulfated GAG and marks an important step in the animal-free production of these molecules.

scholarly journals Optimisation of Recombinant Myrosinase Production in Pichia pastoris

2021 ◽  
Vol 22 (7) ◽  
pp. 3677
Author(s):  
Zuzana Rosenbergová ◽  
Kristína Kántorová ◽  
Martin Šimkovič ◽  
Albert Breier ◽  
Martin Rebroš
Keyword(s):  
Pichia Pastoris ◽  
Plant Defence ◽  
Plant Secondary Metabolites ◽  
Specific Productivity ◽  
Fermentation Conditions ◽  
Threefold Increase ◽  
Dry Cell Weight ◽  
First Time ◽  
Hydrolysis Of ◽  
Dry Cell

Myrosinase is a plant defence enzyme catalysing the hydrolysis of glucosinolates, a group of plant secondary metabolites, to a range of volatile compounds. One of the products, isothiocyanates, proved to have neuroprotective and chemo-preventive properties, making myrosinase a pharmaceutically interesting enzyme. In this work, extracellular expression of TGG1 myrosinase from Arabidopsis thaliana in the Pichia pastoris KM71H (MutS) strain was upscaled to a 3 L laboratory fermenter for the first time. Fermentation conditions (temperature and pH) were optimised, which resulted in a threefold increase in myrosinase productivity compared to unoptimised fermentation conditions. Dry cell weight increased 1.5-fold, reaching 100.5 g/L without additional glycerol feeding. Overall, a specific productivity of 4.1 U/Lmedium/h was achieved, which was 102.5-fold higher compared to flask cultivations.

Quantitative Effects of Oxygen Supply on Mycelia Growth and Extracellular Polysaccharide of Ganoderma tsugae

2012 ◽  
Vol 217-219 ◽  
pp. 975-978
Author(s):  
Nukrob Narkprasom ◽  
Tzou Chi Huang ◽  
Yuan Kuang Guu
Keyword(s):  
Mass Transfer ◽  
Secondary Metabolite ◽  
Submerged Fermentation ◽  
Oxygen Supply ◽  
Cell Mass ◽  
Extracellular Polysaccharide ◽  
Mycelia Growth ◽  
High Level ◽  
Dry Cell

The quantitative effects of oxygen supply in terms of shaking speed and medium volume on the production of mycelia and extracellular polysaccharide (EPS) using Ganoderma tsugae in submerged fermentation were investigated. Mycelia growth required the proper shaking speed at 134 rpm for breaking a larger pellet into several smaller pellets. Furthermore, high level of medium volume for consumption of abundant nutrient is needed for maximum mycelia growth. For EPS, a high agitation is needed to promote a good mass transfer for achieving high product concentrations recovery. The appropriate medium volume was found to be 150.4 mL for aiding to produce a secondary metabolite by promoting the mass transfer of substrates. The dry cell mass (DCM)-EPS diagram of G. tsugae was created from the both production equations which this diagram is an useful tool for submerge fermentation industry for decision about to control the both productions.

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