scholarly journals Astigmatic traction force microscopy (aTFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Di Li ◽  
Huw Colin-York ◽  
Liliana Barbieri ◽  
Yousef Javanmardi ◽  
Yuting Guo ◽  
...  
Keyword(s):  
Total Internal Reflection ◽  
Three Dimensional ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Force Measurements ◽  
Complete Understanding ◽  
Force Microscopy ◽  
Cell Force ◽  
Limited Sensitivity

AbstractQuantifying small, rapidly progressing three-dimensional forces generated by cells remains a major challenge towards a more complete understanding of mechanobiology. Traction force microscopy is one of the most broadly applied force probing technologies but ascertaining three-dimensional information typically necessitates slow, multi-frame z-stack acquisition with limited sensitivity. Here, by performing traction force microscopy using fast single-frame astigmatic imaging coupled with total internal reflection fluorescence microscopy we improve the temporal resolution of three-dimensional mechanical force quantification up to 10-fold compared to its related super-resolution modalities. 2.5D astigmatic traction force microscopy (aTFM) thus enables live-cell force measurements approaching physiological sensitivity.

scholarly journals Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Liliana Barbieri ◽  
Huw Colin-York ◽  
Kseniya Korobchevskaya ◽  
Di Li ◽  
Deanna L. Wolfson ◽  
...  
Keyword(s):  
Resolution Enhancement ◽  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Two Dimensional ◽  
Structured Illumination ◽  
Structured Illumination Microscopy ◽  
Force Microscopy ◽  
Health And Disease ◽  
Spatio Temporal

AbstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.

scholarly journals Epifluorescence-based three-dimensional traction force microscopy

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Lauren Hazlett ◽  
Alexander K. Landauer ◽  
Mohak Patel ◽  
Hadley A. Witt ◽  
Jin Yang ◽  
...  
Keyword(s):  
Spatial Frequency ◽  
Open Source ◽  
Single Particle ◽  
Three Dimensional ◽  
Traction Force ◽  
High Spatial Frequency ◽  
Epifluorescence Microscopy ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Out Of Plane

Abstract We introduce a novel method to compute three-dimensional (3D) displacements and both in-plane and out-of-plane tractions on nominally planar transparent materials using standard epifluorescence microscopy. Despite the importance of out-of-plane components to fully understanding cell behavior, epifluorescence images are generally not used for 3D traction force microscopy (TFM) experiments due to limitations in spatial resolution and measuring out-of-plane motion. To extend an epifluorescence-based technique to 3D, we employ a topology-based single particle tracking algorithm to reconstruct high spatial-frequency 3D motion fields from densely seeded single-particle layer images. Using an open-source finite element (FE) based solver, we then compute the 3D full-field stress and strain and surface traction fields. We demonstrate this technique by measuring tractions generated by both single human neutrophils and multicellular monolayers of Madin–Darby canine kidney cells, highlighting its acuity in reconstructing both individual and collective cellular tractions. In summary, this represents a new, easily accessible method for calculating fully three-dimensional displacement and 3D surface tractions at high spatial frequency from epifluorescence images. We released and support the complete technique as a free and open-source code package.

scholarly journals Three-dimensional Traction Force Microscopy for Studying Cellular Interactions with Biomaterials

2012 ◽  
Vol 4 ◽  
pp. 144-150 ◽  
Author(s):  
J. Notbohm ◽  
J.-H. Kim ◽  
C. Franck ◽  
S. Maskarinec ◽  
D. Tirrell ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Cellular Interactions ◽  
Force Microscopy

Application of 3D Traction Force Microscopy to Mechanotransduction of Cell Clusters

2011 ◽  
Vol 70 ◽  
pp. 21-27 ◽  
Author(s):  
Jacob Notbohm ◽  
Jin Hong Kim ◽  
Anand Asthagiri ◽  
Guruswami Ravichandran
Keyword(s):  
Finite Element ◽  
Three Dimensional ◽  
Traction Force ◽  
Two Dimensions ◽  
Three Dimensions ◽  
Cell Contact ◽  
Traction Force Microscopy ◽  
Inhibition Of Proliferation ◽  
Force Microscopy ◽  
Cell Clusters

With increasing understanding of the important role mechanics plays in cell behavior, the experimental technique of traction force microscopy has grown in popularity over the past decade. While researchers have assumed that cells on a flat substrate apply tractions in only two dimensions, a finite element simulation is discussed here that demonstrates how cells apply tractions in all three dimensions. Three dimensional traction force microscopy is then used to experimentally confirm the finite element results. Finally, the implications that the traction distributions of cell clusters have on the study of inhibition of proliferation due to cell contact and scattering of cells in a cluster are discussed.

scholarly journals Three-Dimensional Traction Force Microscopy: A New Tool for Quantifying Cell-Matrix Interactions

PLoS ONE ◽  
2011 ◽  
Vol 6 (3) ◽  
pp. e17833 ◽  
Author(s):  
Christian Franck ◽  
Stacey A. Maskarinec ◽  
David A. Tirrell ◽  
Guruswami Ravichandran
Keyword(s):  
Three Dimensional ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy ◽  
Cell Matrix ◽  
Matrix Interactions ◽  
Cell Matrix Interactions

scholarly journals Three-Dimensional Quantification of Cellular Traction Forces and Mechanosensing of Thin Substrata by Fourier Traction Force Microscopy

PLoS ONE ◽  
2013 ◽  
Vol 8 (9) ◽  
pp. e69850 ◽  
Author(s):  
Juan C. del Álamo ◽  
Ruedi Meili ◽  
Begoña Álvarez-González ◽  
Baldomero Alonso-Latorre ◽  
Effie Bastounis ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Traction Forces ◽  
Force Microscopy

scholarly journals Fluctuation-Based Super-Resolution Traction Force Microscopy

Nano Letters ◽  
2020 ◽  
Vol 20 (4) ◽  
pp. 2230-2245 ◽  
Author(s):  
Aki Stubb ◽  
Romain F. Laine ◽  
Mitro Miihkinen ◽  
Hellyeh Hamidi ◽  
Camilo Guzmán ◽  
...  
Keyword(s):  
Traction Force ◽  
Super Resolution ◽  
Traction Force Microscopy ◽  
Force Microscopy

scholarly journals Publisher Correction: Epifluorescence-based three-dimensional traction force microscopy

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Lauren Hazlett ◽  
Alexander K. Landauer ◽  
Mohak Patel ◽  
Hadley A. Witt ◽  
Jin Yang ◽  
...  
Keyword(s):  
Three Dimensional ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Force Microscopy

An amendment to this paper has been published and can be accessed via a link at the top of the paper.

scholarly journals The biochemical composition of the actomyosin network sets the magnitude of cellular traction forces

2021 ◽  
Vol 32 (18) ◽  
pp. 1737-1748
Author(s):  
Somanna Kollimada ◽  
Fabrice Senger ◽  
Timothée Vignaud ◽  
Manuel Théry ◽  
Laurent Blanchoin ◽  
...  
Keyword(s):  
Focal Adhesion ◽  
Biochemical Composition ◽  
Force Production ◽  
Traction Force ◽  
Traction Force Microscopy ◽  
Traction Forces ◽  
Force Microscopy ◽  
Cell Force

The endogenous content of proteins associated with force production and the resultant traction forces were quantified in the same cells using a new traction force-microscopy assay. Focal adhesion size correlated with force in stationary cells. Relative numbers of motors and cross-linkers per actin required an optimum to maximize cell force production.

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