scholarly journals Fibroblast fusion to the muscle fiber regulates myotendinous junction formation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Wesal Yaseen ◽  
Ortal Kraft-Sheleg ◽  
Shelly Zaffryar-Eilot ◽  
Shay Melamed ◽  
Chengyi Sun ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Musculoskeletal System ◽  
Smooth Transition ◽  
Common Mechanism ◽  
Myotendinous Junction ◽  
Body Movements ◽  
Dual Identity ◽  
Junction Formation ◽  
Myogenic Program ◽  
Developing Muscle

AbstractVertebrate muscles and tendons are derived from distinct embryonic origins yet they must interact in order to facilitate muscle contraction and body movements. How robust muscle tendon junctions (MTJs) form to be able to withstand contraction forces is still not understood. Using techniques at a single cell resolution we reexamine the classical view of distinct identities for the tissues composing the musculoskeletal system. We identify fibroblasts that have switched on a myogenic program and demonstrate these dual identity cells fuse into the developing muscle fibers along the MTJs facilitating the introduction of fibroblast-specific transcripts into the elongating myofibers. We suggest this mechanism resulting in a hybrid muscle fiber, primarily along the fiber tips, enables a smooth transition from muscle fiber characteristics towards tendon features essential for forming robust MTJs. We propose that dual characteristics of junctional cells could be a common mechanism for generating stable interactions between tissues throughout the musculoskeletal system.

scholarly journals Fibroblast fusion to the muscle fiber regulates myotendinous junction formation

2020 ◽  
Author(s):  
Wesal Yaseen-Badarneh ◽  
Ortal Kraft-Sheleg ◽  
Shelly Zaffryar-Eilot ◽  
Shay Melamed ◽  
Chengyi Sun ◽  
...  
Keyword(s):  
Muscle Fiber ◽  
Musculoskeletal System ◽  
Smooth Transition ◽  
Common Mechanism ◽  
Myotendinous Junction ◽  
Body Movements ◽  
Dual Identity ◽  
Junction Formation ◽  
Myogenic Program ◽  
Developing Muscle

SummaryVertebrate muscles and tendons are derived from distinct embryonic origins yet they must interact in order to facilitate muscle contraction and body movements. How robust muscle tendon junctions (MTJs) form to be able to withstand contraction forces is still not understood. Using techniques at a single cell resolution we reexamined the classical view of distinct identities for the tissues composing the musculoskeletal system. We identified fibroblasts that have switched on a myogenic program and demonstrate these dual identity cells fuse into the developing muscle fibers along the MTJs facilitating the introduction of fibroblast-specific transcripts into the elongating myofibers. We suggest this mechanism resulting in a hybrid muscle fiber, primarily along the fiber tips, enables a smooth transition from muscle fiber characteristics towards tendon features essential for forming robust MTJs. We propose that dual characteristics of junctional cells could be a common mechanism for generating stable interactions between tissues throughout the musculoskeletal system.

Epidermal tendon cells require Broad Complex function for correct attachment of the indirect flight muscles in Drosophila melanogaster

1999 ◽  
Vol 112 (22) ◽  
pp. 4051-4065 ◽  
Author(s):  
D.J. Sandstrom ◽  
L.L. Restifo
Keyword(s):  
Complex Function ◽  
Myotendinous Junction ◽  
Wild Type ◽  
Muscle Attachment ◽  
Attachment Sites ◽  
Tendon Cells ◽  
Flight Muscles ◽  
Broad Complex ◽  
Developing Muscle ◽  
Dorsal Epidermis

Drosophila Broad Complex, a primary response gene in the ecdysone cascade, encodes a family of zinc-finger transcription factors essential for metamorphosis. Broad Complex mutations of the rbp complementation group disrupt attachment of the dorsoventral indirect flight muscles during pupal development. We previously demonstrated that isoform BRC-Z1 mediates the muscle attachment function of rbp(+) and is expressed in both developing muscle fibers and their epidermal attachment sites. We now report two complementary studies to determine the cellular site and mode of action of rbp(+) during maturation of the myotendinous junctions of dorsoventral indirect flight muscles. First, genetic mosaics, produced using the paternal loss method, revealed that the muscle attachment phenotype is determined primarily by the genotype of the dorsal epidermis, with the muscle fiber and the ventral epidermis exerting little or no influence. When the dorsal epidermis was mutant, the vast majority of muscles detached or chose ectopic attachment sites, regardless of the muscle genotype. Conversely, wild-type dorsal epidermis could support attachment of mutant muscles. Second, ultrastructural analysis corroborated and extended these results, revealing defective and delayed differentiation of rbp mutant epidermal tendon cells in the dorsal attachment sites. Tendon cell processes, the stress-bearing links between the epidermis and muscle, were reduced in number and showed delayed appearance of microtubule bundles. In contrast, mutant muscle and ventral epidermis resembled the wild type. In conclusion, BRC-Z1 acts in the dorsal epidermis to ensure differentiation of the myotendinous junction. By analogy with the cell-cell interaction essential for embryonic muscle attachment, we propose that BRC-Z1 regulates one or more components of the epidermal response to a signal from the developing muscle.

scholarly journals The kinectome: a comprehensive kinematic map of human motion in health and disease

2021 ◽  
Author(s):  
Emahnuel Troisi Lopez ◽  
Pierpaolo Sorrentino ◽  
Marianna Liparoti ◽  
Roberta Minino ◽  
Anna Carotenuto ◽  
...  
Keyword(s):  
Musculoskeletal System ◽  
Human Movement ◽  
Human Motion ◽  
High Variability ◽  
Body Parts ◽  
Healthy Individuals ◽  
Body Movements ◽  
Human Kinematics ◽  
Health And Disease ◽  
Network Approaches

Effective human movement requires the coordinated participation of the whole musculoskeletal system. Here we propose to represent the human body movements as a network (that we named "kinectome"), where nodes are body parts, and edges are defined as the correlations of the accelerations between each pair of body parts during gait. We apply this framework in healthy individuals and patients with Parkinson's disease (PD). The network dynamics in Parkinson's display high variability, as conveyed by the high variance and the modular structure in the patients' kinectomes. Furthermore, our analysis identified a set of anatomical elements that are specifically related to the balance impairment in PD. Furthermore, each participant could be identified basedon its kinectome patterns, akin to a "fingerprint" of movement, confirming that our approach captures relevant features of gait. We hope that applying network approaches to human kinematics yields new insights to characterize human movement.

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