Dynamic formation of microenvironments at the myotendinous junction correlates with muscle fiber morphogenesis in zebrafish

AbstractVertebrate muscles and tendons are derived from distinct embryonic origins yet they must interact in order to facilitate muscle contraction and body movements. How robust muscle tendon junctions (MTJs) form to be able to withstand contraction forces is still not understood. Using techniques at a single cell resolution we reexamine the classical view of distinct identities for the tissues composing the musculoskeletal system. We identify fibroblasts that have switched on a myogenic program and demonstrate these dual identity cells fuse into the developing muscle fibers along the MTJs facilitating the introduction of fibroblast-specific transcripts into the elongating myofibers. We suggest this mechanism resulting in a hybrid muscle fiber, primarily along the fiber tips, enables a smooth transition from muscle fiber characteristics towards tendon features essential for forming robust MTJs. We propose that dual characteristics of junctional cells could be a common mechanism for generating stable interactions between tissues throughout the musculoskeletal system.

Download Full-text

Fibroblast fusion to the muscle fiber regulates myotendinous junction formation

10.1101/2020.07.20.213199 ◽

2020 ◽

Author(s):

Wesal Yaseen-Badarneh ◽

Ortal Kraft-Sheleg ◽

Shelly Zaffryar-Eilot ◽

Shay Melamed ◽

Chengyi Sun ◽

...

Keyword(s):

Muscle Fiber ◽

Musculoskeletal System ◽

Smooth Transition ◽

Common Mechanism ◽

Myotendinous Junction ◽

Body Movements ◽

Dual Identity ◽

Junction Formation ◽

Myogenic Program ◽

Developing Muscle

SummaryVertebrate muscles and tendons are derived from distinct embryonic origins yet they must interact in order to facilitate muscle contraction and body movements. How robust muscle tendon junctions (MTJs) form to be able to withstand contraction forces is still not understood. Using techniques at a single cell resolution we reexamined the classical view of distinct identities for the tissues composing the musculoskeletal system. We identified fibroblasts that have switched on a myogenic program and demonstrate these dual identity cells fuse into the developing muscle fibers along the MTJs facilitating the introduction of fibroblast-specific transcripts into the elongating myofibers. We suggest this mechanism resulting in a hybrid muscle fiber, primarily along the fiber tips, enables a smooth transition from muscle fiber characteristics towards tendon features essential for forming robust MTJs. We propose that dual characteristics of junctional cells could be a common mechanism for generating stable interactions between tissues throughout the musculoskeletal system.

Download Full-text

Scanning Electron Microscopic Study of the Muscle Fiber Ends at the Myotendinous Junction in the Posterior Cricoarytenoid and Cricothyroid Muscles in Rats

CrossRef Listing of Deleted DOIs ◽

10.1080/000164801317166853 ◽

2001 ◽

Vol 121 (8) ◽

pp. 953-956 ◽

Cited By ~ 4

Author(s):

Masamitsu Hyodo ◽

Seiji Kawakita ◽

Junzo Desaki

Keyword(s):

Microscopic Study ◽

Electron Microscopic Study ◽

Muscle Fiber ◽

Myotendinous Junction ◽

Electron Microscopic ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Posterior Cricoarytenoid ◽

Scanning Electron

Download Full-text

Morphodifferentiation of Skeletal Muscle Fiber Ends at the Myotendinous Junction in the Postnatal Chinese Hamster:A Scanning Electron Microscopic Study.

Archives of Histology and Cytology ◽

10.1679/aohc.61.89 ◽

1998 ◽

Vol 61 (1) ◽

pp. 89-92 ◽

Cited By ~ 2

Author(s):

Yoji NAGANO ◽

Yoshiro MATSUDA ◽

Junzo DESAKI ◽

Sadaaki OKI ◽

Kenji KITAOKA ◽

...

Keyword(s):

Skeletal Muscle ◽

Microscopic Study ◽

Electron Microscopic Study ◽

Muscle Fiber ◽

Skeletal Muscle Fiber ◽

Myotendinous Junction ◽

Electron Microscopic ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Scanning Electron

Download Full-text

Scanning Electron Microscopic Study of the Muscle Fiber Ends at the Myotendinous Junction in the Posterior Cricoarytenoid and Cricothyroid Muscles in Rats

Acta Oto-Laryngologica ◽

10.1080/00016480127364 ◽

2001 ◽

Vol 121 (8) ◽

pp. 953-956

Author(s):

Masamitsu Hyodo, Seiji Kawakita, Junzo Desaki

Keyword(s):

Microscopic Study ◽

Electron Microscopic Study ◽

Muscle Fiber ◽

Myotendinous Junction ◽

Electron Microscopic ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Posterior Cricoarytenoid ◽

Scanning Electron

Download Full-text

Formation of highly organized skeletal muscle fibers in vitro. Comparison with muscle development in vivo

Journal of Cell Science ◽

10.1242/jcs.102.3.643 ◽

1992 ◽

Vol 102 (3) ◽

pp. 643-652 ◽

Cited By ~ 2

Author(s):

S. Swasdison ◽

R. Mayne

Keyword(s):

Skeletal Muscle ◽

Extracellular Matrix ◽

Muscle Fiber ◽

Muscle Development ◽

Muscle Fibers ◽

Myotendinous Junction ◽

Type Iv

Two methods were developed in which long-term cultures of quail skeletal muscle were established so that all of the muscle fibers develop in a highly oriented manner. The muscle fibers became spontaneously and vigorously contractile and established strong connections with the extracellular matrix at their ends that closely duplicate the structure of the myotendinous junction. A continuous basal lamina was formed around each muscle fiber that contained type IV collagen, laminin and heparan sulfate proteoglycan. With one of the methods, an extensive extracellular matrix developed around each muscle fiber that was highly organized with the formation of a distinctive epimysium, perimysium and endomysium. Analysis of the cultures by both methods for different isoforms of myosin showed expression of an adult form of myosin by some of the muscle cells. The results therefore demonstrate that muscle development in the present culture systems proceeds extensively for several weeks. It will now be possible to investigate directly the structure of the connections between muscle fibers and the extracellular matrix.

Download Full-text

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100124288 ◽

1992 ◽

Vol 50 (1) ◽

pp. 776-777

Author(s):

Joachim R. Sommer ◽

Teresa High ◽

Betty Scherer ◽

Isaiah Taylor ◽

Rashid Nassar

Keyword(s):

Skeletal Muscle ◽

High Resolution ◽

Action Potential ◽

Muscle Fiber ◽

Freeze Fracture ◽

Freeze Substitution ◽

Electrical Field Stimulation ◽

Skeletal Muscle Fiber ◽

Freeze Dried ◽

Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

Download Full-text