In this work, the matrix-assisted laser desorption and ionization
time-of-flight (MALDI-TOF) mass spectra of two cationic complexes, i.e.,
[PdCl(dien)]Cl and [Ru(en)2Cl2]Cl, acquired under different conditions were
analyzed. The spectra were recorded with three matrices with or without
trifluoroacetic acid (TFA), i.e., two traditional matrices, i.e.,
2,5-dihydroxybenzoic acid and ?-cyano-hydroxycinnamic acid, and one
flavonoid, quercetin. The spectra acquired with quercetin appeared to be the
simplest, whereas in the spectra obtained with other matrices, peaks arising
either from the addition of matrix molecules or from the fragmentation
products were detectable. Addition of TFA did not complicate the spectra of
the Pd(II) and Ru(III) complexes when the traditional matrices were used. On
the other hand, the spectra of Pd complex were simpler, whereas the addition
of TFA in the case of the Ru complex resulted in a higher number of peaks,
some of which could not be identified. Taken together, the results of this
study once more emphasize the differences arising in the MALDI-TOF mass
spectra of transition metal complexes in dependence on the applied matrix.
Direct antimicrobial resistance prediction from clinical MALDI-TOF mass spectra using machine learning
