Analysis of VSV pseudotype virus infection mediated by rubella virus envelope proteins

Background: Rubella infection occurring during early pregnancy results in congenital rubella syndrome (CRS). WHO estimates that worldwide more than 100,000 children are born with CRS each year and most of them are in the developing countries. For assessing population immunity against rubella, sero-surveys are generally recommended among adolescent girls and reproductive age female. In India, sero-surveys conducted by different authors have indicated that about 10-30% of adolescent females are susceptible to rubella infection. Adolescent girls are selected because they are at a critical stage of child bearing age and their immunity against Rubella infection is the particular area of interest. objective of this study was to estimate the sero-prevalence of unvaccinated adolescent girls susceptible to Rubella virus infection attending a tertiary care hospital of Patna and then accordingly counsel for vaccination.Methods: A total 150 adolescent girls in the age group of 10-19 years who had not received MMR vaccine were included in the study. Serum IgG antibody titer for rubella was estimated by the ELISA method.Results: A total 65.33% of the adolescent girls were found to be rubella seropositive and (34.67%) were seronegative. The urban adolescent girls had a higher seropositivity of 85.2% as compared to rural adolescent girls.Conclusions: The study indicates that a substantial number of adolescents (34.67%) are seronegative and hence susceptible to rubella infection.

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Effects of Rubella Virus Infection on Islet Function

Current Topics in Microbiology and Immunology - The Role of Viruses and the Immune System in Diabetes Mellitus ◽

10.1007/978-3-642-75239-1_5 ◽

1990 ◽

pp. 63-74 ◽

Cited By ~ 1

Author(s):

E. J. Rayfield

Keyword(s):

Virus Infection ◽

Rubella Virus ◽

Islet Function ◽

Rubella Virus Infection

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The Hepatitis B Virus Envelope Proteins: Molecular Gymnastics Throughout the Viral Life Cycle

Annual Review of Virology ◽

10.1146/annurev-virology-092818-015508 ◽

2020 ◽

Vol 7 (1) ◽

pp. 263-288 ◽

Cited By ~ 1

Author(s):

Stefan Seitz ◽

Jelena Habjanič ◽

Anne K. Schütz ◽

Ralf Bartenschlager

Keyword(s):

Life Cycle ◽

Hepatitis B ◽

Reverse Transcription ◽

Hepatitis B Surface Antigen ◽

Viral Life Cycle ◽

Envelope Proteins ◽

Confined Space ◽

Virus Envelope ◽

Virion Assembly ◽

Multiple Conformations

New hepatitis B virions released from infected hepatocytes are the result of an intricate maturation process that starts with the formation of the nucleocapsid providing a confined space where the viral DNA genome is synthesized via reverse transcription. Virion assembly is finalized by the enclosure of the icosahedral nucleocapsid within a heterogeneous envelope. The latter contains integral membrane proteins of three sizes, collectively known as hepatitis B surface antigen, and adopts multiple conformations in the course of the viral life cycle. The nucleocapsid conformation depends on the reverse transcription status of the genome, which in turn controls nucleocapsid interaction with the envelope proteins for virus exit. In addition, after secretion the virions undergo a distinct maturation step during which a topological switch of the large envelope protein confers infectivity. Here we review molecular determinants for envelopment and models that postulate molecular signals encoded in the capsid scaffold conducive or adverse to the recruitment of envelope proteins.

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Single point mutation in tick-borne encephalitis virus prM protein induces a reduction of virus particle secretion

Journal of General Virology ◽

10.1099/vir.0.80169-0 ◽

2004 ◽

Vol 85 (10) ◽

pp. 3049-3058 ◽

Cited By ~ 36

Author(s):

Kentarou Yoshii ◽

Akihiro Konno ◽

Akiko Goto ◽

Junko Nio ◽

Mayumi Obara ◽

...

Keyword(s):

Point Mutation ◽

Single Point ◽

Expression System ◽

Envelope Proteins ◽

Viral Envelope ◽

Single Point Mutation ◽

Virus Envelope ◽

Electron Microscopic ◽

Transport Pathway ◽

Tick Borne Encephalitis

Flaviviruses are assembled to bud into the lumen of the endoplasmic reticulum (ER) and are secreted through the vesicle transport pathway. Virus envelope proteins play important roles in this process. In this study, the effect of mutations in the envelope proteins of tick-borne encephalitis (TBE) virus on secretion of virus-like particles (VLPs), using a recombinant plasmid expression system was analysed. It was found that a single point mutation at position 63 in prM induces a reduction in secretion of VLPs. The mutation in prM did not affect the folding of the envelope proteins, and chaperone-like activity of prM was maintained. As observed by immunofluorescence microscopy, viral envelope proteins with the mutation in prM were scarce in the Golgi complex, and accumulated in the ER. Electron microscopic analysis of cells expressing the mutated prM revealed that many tubular structures were present in the lumen. The insertion of the prM mutation at aa 63 into the viral genome reduced the production of infectious virus particles. This data suggest that prM plays a crucial role in the virus budding process.

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