Tobacco-Free Cigarette Smoke Exposure Induces Anxiety and Panic-Related Behaviours in Male Wistar Rats

BACKGROUND: Cigarette smoke has a variety of dangerous chemicals and free radicals that can potentially cause infertility. One of the plants that are reported to have medicinal properties and have active compounds as antioxidants is Binahong (Anredera cordifolia (Ten.) Steenis). AIM: This study aims to investigate the effectiveness of A. cordifolia leaf extract (ACLE) in increasing the fertility of male Wistar rats after exposure to cigarette smoke. METHODS: Twenty-four adult male rats were divided into six groups (age 8–10 weeks, weight 200–250 g): Group 1 (normal control) received aquades (1 ml/day) without being given cigarette smoke, Group 2 received exposure to smoke without being given ACLE, Group 3 received cigarette smoke exposure + 25 mg/kg ACLE, Group 4 received cigarette smoke exposure + 50 mg/kg ACLE, Group 5 received cigarette smoke exposure + 75 mg/kg ACLE, and Group 6 received exposure to cigarette smoke + 100 mg/kg of ACLE. To produce smoke from cigarettes and expose rats to the smoke, a Smoke chamber is used. Rats in the treatment group were exposed to cigarette smoke for 2 weeks (40–60 s daily for 6 days each week). The evaluation of male rat fertility was carried out by measuring body weight, genital weight (testis and epididymis), and spermatozoa spermogram (velocity, motility, morphology, and total number). RESULTS: The least significance different test results showed no significant difference in the rate of weight gain between treatments, standard control, and negative control groups. The average testicular weight of rats exposed to cigarette smoke for 14 days was significantly different from normal controls and doses of 50, 75, and 100 mg/kg body weight. However, at a dose of 25 mg/kg of body weight had not shown a significant increase compared to control. The average spermatozoa velocity reached 12.43 mm2/s and 13.36 mm2/s. The spermatozoa velocity increased significantly at a dose of 100 mg/kg body weight. CONCLUSIONS: ACLE at a dose of 100 mg/kg body weight was effective in increasing the fertility of male Wistar rats exposed to cigarette smoke.

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Post chronic cigarette smoke exposure and assessment of learning, memory and asymptomatic level of performance in Wistar rats

Bayero Journal of Pure and Applied Sciences ◽

10.4314/bajopas.v8i2.10 ◽

2016 ◽

Vol 8 (2) ◽

pp. 46

Author(s):

IM Badamasi ◽

IU Lawal

Keyword(s):

Cigarette Smoke ◽

Wistar Rats ◽

Smoke Exposure ◽

Cigarette Smoke Exposure ◽

Assessment Of Learning

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Cigarette Smoke Induces Colorectal Carcinogenesis in Wistar Rats by Decreasing The Expression of APC, MSH2 and MLH1

Biosaintifika Journal of Biology & Biology Education ◽

10.15294/biosaintifika.v9i1.8439 ◽

2017 ◽

Vol 9 (1) ◽

pp. 66 ◽

Cited By ~ 1

Author(s):

Dody Novrial ◽

Ignatius Riwanto ◽

Sutiman Bambang Sumitro ◽

Indra Wijaya

Keyword(s):

Colorectal Cancer ◽

Cigarette Smoke ◽

Wistar Rats ◽

Wistar Rat ◽

Colorectal Carcinogenesis ◽

Cigarette Smoke Exposure ◽

Control Group ◽

Time Duration ◽

Epithelial Morphology ◽

Male Wistar Rats

<p>Colorectal carcinogenesis induced by cigarette smoke requires at least 30-40 years. This long time duration causes an animal research conducted becomes relevant. This research was carried out to observe colorectal carcinogenesis due to cigarette smoke exposure in Wistar Rat. The observations focused on changes in epithelial morphology and expression of APC, MSH2 and MLH1. Twenty male Wistar rats inbreed strain were randomly allocated into control group and experimental group exposure to cigarettes smoke for 14 weeks and 28 weeks sequentially. Colorectal epithelial morphology was assessed on the histopathology examination, whereas the expression of APC, MSH2 and MLH1 was assessed on aspect of immunohistochemistry. The comparative analysis between the two groups was performed using non-parametric Mann-Whitney U test. Histology of colorectal epithelium showed pattern of colitis associated cancer that was significant both in 14 weeks and 28 weeks of treatment. This research indicated negative expression of APC, MSH2 and MLH1 in the colorectal cancer that were significant at 28 weeks of exposure. This research implies that chronic exposure to cigarette smoke can induce colitis associated colorectal cancer through decreased expression of APC, MSH2 and MLH1. </p>

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Acute cigarette smoke exposure induces oxidative damage and inflammation in Wistar rats: impact on lungs and erythrocytes

African Journal of Biological Sciences ◽

10.33472/afjbs.3.1.2021.120-128 ◽

2021 ◽

Vol 3 (1) ◽

pp. 120-128

Author(s):

Shahdat Hossain ◽

Marzan Sarkar ◽

Sujan Bhowmick ◽

Jakir Hussain ◽

Mahmudul Hasan ◽

...

Keyword(s):

Oxidative Damage ◽

Cigarette Smoke ◽

Wistar Rats ◽

Smoke Exposure ◽

Cigarette Smoke Exposure

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EFEK EKSTRAK MELON (Cucumis melo) dan GliadinTERHADAP KADAR Hb DAN HbCO TIKUS WISTAR JANTAN YANG DIPAPAR ASAP ROKOK

The Indonesian Journal of Public Health ◽

10.20473/ijph.v11i1.2016.78-88 ◽

2017 ◽

Vol 11 (1) ◽

pp. 78

Author(s):

Yuyun Erlina Susanti ◽

Bambang Wirjatmadi

Keyword(s):

Treatment Group ◽

Cigarette Smoke ◽

Cucumis Melo ◽

Wistar Rats ◽

Cigarette Smoke Exposure ◽

Control Group ◽

P Value ◽

Control Group Design ◽

Significant Difference ◽

Male Wistar Rats

The exposure of cigarette smoke produce CO bond in hemoglobin. Melon (Cucumis melo) and gliadin contains of antioxidants that prevent tissue damage. The aim of this study was to analyze the difference of hemoglobin and carboxyhemoglobin concentration in male Wistar rats were exposed to cigarette smoke and had been treated melon extract (Cucumis melo) and gliadin. The study was a laboratory experimental design, using Post Test Control Group Design Research and RAL method (Complete Randomize Design). The sample in this study were 25 male Wistar rats aged 3 months. The research was divided into 5 groups with 5 different treatment : control group, the treatment group were given exposure to cigarette smoke, the treatment group were given of cigarette smoke exposure and melon extract (Cucumis melo) and gliadin dose of 3 IU / day, 4.5 IU / day, and 9 IU / day.The subject were divided into 5 groups each treated for 28 day. The sample size used Federer formula. The collection of data was obtained from the results of laboratory tests to hemoglobin and Carboxyhemoglobin. Hb and HbCO data collected and analyzed by Manova test at 95% confidence level. The results showed significant difference in average Hb and HbCO concentration (p-value = 0.000) between male Wistar rats treated and not treated with melon extract (Cucumis melo) and gliadin. Melon extract has an effect on the decline of HbCO concentration due to exposure to cigarette smoke.Keywords:smoke, melon extract, Hb, HbCO

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Association of diabetes and cigarette smoke exposure on the glycemia and liver glycogen of pregnant Wistar rats

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502008000600002 ◽

2008 ◽

Vol 23 (6) ◽

pp. 481-485 ◽

Cited By ~ 4

Author(s):

Yuri Karen Sinzato ◽

Paula Helena Ortiz Lima ◽

Carlos Eduardo Meirelles dos Santos ◽

Kleber Eduardo de Campos ◽

Marilza Vieira Cunha Rudge ◽

...

Keyword(s):

Cigarette Smoke ◽

Wistar Rats ◽

Liver Glycogen ◽

Glycogen Storage ◽

Smoke Exposure ◽

Cigarette Smoke Exposure ◽

Maternal Weight ◽

Adult Rats ◽

Stop Smoking ◽

Severe Diabetes

PURPOSE: To evaluate cigarette smoke exposure and/or diabetes association effects on the glycemia and liver glycogen levels of pregnant Wistar rats. METHODS: 60 adult rats were randomly distributed into (n=10/group): non-diabetic exposed to filtered air (G1); non-diabetic exposed to cigarette smoke only before pregnancy (G2); non-diabetic exposed to cigarette smoke before and during pregnancy (G3); diabetic exposed to filtered air (G4); diabetic exposed to cigarette smoke only before pregnancy (G5), and diabetic exposed to cigarette smoke before and during pregnancy (G6). Glycemia was determined at days 0 and 21 of pregnancy. Liver samples were collected for liver glycogen determinations. RESULTS: At day 21 of pregnancy, glycemia was higher in G5 and G6 compared to G4 group. G2 (2.43±0.43), G3 (3.20±0.49), G4 (2.62±0.34), G5 (2.65±0.27) and G6 groups (1.94±0.35) presented decreased liver glycogen concentrations compared to G1 (4.20±0.18 mg/100mg liver tissue) (p<0.05). G5 and G6 groups presented decreased maternal weight gain and litter weight. CONCLUSIONS: Severe diabetes and cigarette smoke exposure, alone or associated, caused impairment in liver glycogen storage at term pregnancy. Due to the fact that liver glycogen storages were considered determinant for glucose tolerance, it is relevant to point out a rigid clinical glycemic control and to stop smoking so earlier in pregnancy programming.

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