scholarly journals Fibulin-3 knockdown inhibits cervical cancer cell growth and metastasis in vitro and in vivo

2018 ◽  
Vol 8 (1) ◽  
Author(s):  
Juan Li ◽  
Chen Qi ◽  
Xia Liu ◽  
Changzhong Li ◽  
Jie Chen ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Cervical Cancer Cell ◽  
Cancer Cell Growth

scholarly journals HOXB4 inhibits the proliferation and tumorigenesis of cervical cancer cells by downregulating the activity of Wnt/β-catenin signaling pathway

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dan Lei ◽  
Wen-Ting Yang ◽  
Peng-Sheng Zheng
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Signaling Pathway ◽  
Cancer Cell ◽  
Tumor Formation ◽  
Cervical Cancer Cell ◽  
Cancer Cell Growth ◽  
Bioinformatics Analyses

AbstractHomeobox B4 (HOXB4), which belongs to the homeobox (HOX) family, possesses transcription factor activity and has a crucial role in stem cell self-renewal and tumorigenesis. However, its biological function and exact mechanism in cervical cancer remain unknown. Here, we found that HOXB4 was markedly downregulated in cervical cancer. We demonstrated that HOXB4 obviously suppressed cervical cancer cell proliferation and tumorigenic potential in nude mice. Additionally, HOXB4-induced cell cycle arrest at the transition from the G0/G1 phase to the S phase. Conversely, loss of HOXB4 promoted cervical cancer cell growth both in vitro and in vivo. Bioinformatics analyses and mechanistic studies revealed that HOXB4 inhibited the activity of the Wnt/β-catenin signaling pathway by direct transcriptional repression of β-catenin. Furthermore, β-catenin re-expression rescued HOXB4-induced cervical cancer cell defects. Taken together, these findings suggested that HOXB4 directly transcriptional repressed β-catenin and subsequently inactivated the Wnt/β-catenin signaling pathway, leading to significant inhibition of cervical cancer cell growth and tumor formation.

scholarly journals Knockdown of hTERT by siRNA inhibits cervical cancer cell growth in vitro and in vivo

2014 ◽  
Vol 45 (3) ◽  
pp. 1216-1224 ◽  
Author(s):  
YING-AI SHI ◽  
QIANG ZHAO ◽  
LI-HONG ZHANG ◽  
WEI DU ◽  
XUE-YAO WANG ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Cervical Cancer Cell ◽  
Cancer Cell Growth

scholarly journals Inhibition of cervical cancer cell growth in vitro and in vivo with dual shRNAs

2010 ◽  
Vol 18 (3) ◽  
pp. 219-227 ◽  
Author(s):  
W Gu ◽  
E Payne ◽  
S Sun ◽  
M Burgess ◽  
N A J McMillan
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Cancer Cell ◽  
Cervical Cancer Cell ◽  
Cancer Cell Growth

Anti-cancer effect of adenovirus p53 on human cervical cancer cell growth in vitro and in vivo

2004 ◽  
Vol 14 (2) ◽  
pp. 322-332 ◽  
Author(s):  
W. S. Ahn ◽  
S. M. Bae ◽  
J. M. Lee ◽  
S. E. Namkoong ◽  
J. Y. Yoo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Growth Inhibition ◽  
Cancer Cell ◽  
Cervical Cancer Cell ◽  
Transduction Efficiency ◽  
Cell Growth Inhibition ◽  
P53 Expression

To evaluate anti-tumor effects of recombinant adenovirus p53, time-course p53, E6 expression, and cell growth inhibition were investigated in vitro and in vivo using cervical cancer cell lines such as CaSki, SiHa, HeLa, HeLaS3, C33A, and HT3. The cell growth inhibition was studied via cell count assay, MTT assay and neutral red assay. After transfecting AdCMVp53 into SiHa cells-xenografted nude mice, the transduction efficiency and anti-tumor effect were investigated for a month. The results showed that adenoviral p53 expression induced significant growth suppression on the cancer cells, in which E6 transcript was strongly repressed, and that the expression of p53 and E6 were remarkably dependent on each cell type. The transduction efficiency was highly maintained in vivo as well as in vitro, and the size of tumor was remarkably decreased in comparison with AdCMVLacZ control. The results suggest that the adenovirus-mediated p53 gene transfection was done very effectively in vitro and in vivo experiment, and the cell growth was suppressed via p53-dependent apoptotic cell death, and that the anti-tumor effect could be related to E6 and p53 expression pattern.

scholarly journals Expression of the H19 Oncofetal Gene in Premalignant Lesions of Cervical Cancer: A Potential Targeting Approach for Development of Nonsurgical Treatment of High-Risk Lesions

2013 ◽  
Vol 2013 ◽  
pp. 1-7 ◽  
Author(s):  
Tomer Feigenberg ◽  
Ofer N. Gofrit ◽  
Galina Pizov ◽  
Avraham Hochberg ◽  
Abraham Benshushan
Keyword(s):  
Cervical Cancer ◽  
Cell Growth ◽  
Cell Lines ◽  
Cancer Cell ◽  
Premalignant Lesions ◽  
Cervical Cancer Cell ◽  
Two Samples ◽  
H19 Gene

Background. Recent data suggest a role for H19 gene in promoting cancer transformation and progression. Cervical cancer, progresses from high-grade lesions (CIN3). At present, it is unclear if CIN lesions express H19. Objectives. To determine H19 expression in patient samples of CIN3 as well as the ability of a construct in which the promoter from the H19 gene drives expression of the diphtheria toxin A chain (DTA) to inhibit cervical cancer cell growth in vitro. Methods. H19 transcript levels were evaluated on 10 biopsies of CIN3 using in situ hybridization. PCR was used to examine H19 expression in cervical cancer cell lines and in two samples from a patient with cervical carcinoma. Cell lines were transfected with H19-DTA to determine its impact on cell number. Results. H19 gene was expressed in the area of CIN3 in 9 out of 10 samples. RT-PCR indicated expression of H19 in cervical cancer samples and in one of the three cell lines examined. Transfection of all cell lines with H19-DTA vector resulted in inhibited cell growth. Conclusions. H19 is expressed in the majority of CIN3 samples. These results suggest that most CIN3 lesions could be targeted by H19-DTA. Further in vivo preclinical studies are thus warranted.

794: Valproic Acid Inhibits Prostate Cancer Cell Growth in Vitro and in Vivo

2006 ◽  
Vol 175 (4S) ◽  
pp. 257-257
Author(s):  
Jennifer Sung ◽  
Qinghua Xia ◽  
Wasim Chowdhury ◽  
Shabana Shabbeer ◽  
Michael Carducci ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Valproic Acid ◽  
Cell Growth ◽  
Cancer Cell ◽  
Prostate Cancer Cell ◽  
Cancer Cell Growth
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