In-vitro evaluation of apoptotic effect of OEO and thymol in 2D and 3D cell cultures and the study of their interaction mode with DNA

Two-dimensional (2D) monolayer cell cultures are the most common in vitro models for mechanistic studies on the toxicity of engineered nanoparticles (NPs).

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Correction: Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures

RSC Advances ◽

10.1039/d0ra90118k ◽

2020 ◽

Vol 10 (72) ◽

pp. 44397-44397

Author(s):

Zhipan Wu ◽

Rongfa Guan ◽

Miao Tao ◽

Fei Lyu ◽

Guozhou Cao ◽

...

Keyword(s):

Zinc Oxide ◽

Cell Cultures ◽

Zinc Oxide Nanoparticles ◽

Oxide Nanoparticles ◽

3D Cell Cultures ◽

2D And 3D

Correction for ‘Assessment of the toxicity and inflammatory effects of different-sized zinc oxide nanoparticles in 2D and 3D cell cultures’ by Zhipan Wu, Rongfa Guan, Miao Tao et al., RSC Adv., 2017, 7, 12437–12445, DOI: 10.1039/C6RA27334C.

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Biological high throughput screening of 2D and 3D cell cultures for future industrial up-scaling

2015 IEEE International Conference on Automation Science and Engineering (CASE) ◽

10.1109/coase.2015.7294316 ◽

2015 ◽

Cited By ~ 2

Author(s):

C. Gallert ◽

R. Lehmann ◽

T. Roddelkopf ◽

S. Junginger ◽

K. Thurow

Keyword(s):

Cell Cultures ◽

High Throughput ◽

High Throughput Screening ◽

3D Cell Cultures ◽

2D And 3D ◽

Up Scaling

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Chitosan biopolymer: Alternative adhesion factor and scaffold matrix for 2D and 3D neuronal cultures

Biomedical Science and Engineering ◽

10.4081/bse.2019.107 ◽

2020 ◽

Author(s):

Donatella Di Lisa ◽

Mariateresa Tedesco ◽

Elena Dellacasa ◽

Mattia Pesce ◽

Tiziano Catelani ◽

...

Keyword(s):

Cell Cultures ◽

Culture Media ◽

In Vitro Models ◽

Neuronal Cultures ◽

Culture Techniques ◽

Chitosan Biopolymer ◽

2D And 3D ◽

Adhesion Factor

The increase of different types of cell cultures, which can be used for the in vitro studies of physiological and/or pathological processes, has introduced the need to improve culture techniques through the use of materials and culture media that promote growth, recreating a cellular micro-environment that can be asserted in in vivo condition. The standard methods for the functionalization of supports used for cell cultures are based on the use of synthetic or natural biopolymers, which generally have high costs, such as poly-lysine and polyornithine. The aim of this work is to demonstrate the alternative use of the polysaccharide chitosan as adhesion factor and structural component for 2D/3D neuronal cultures. Thanks to its versatility, it could be easily functionalized for the fabrication of personalized of in vitro models

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Ratiometric Nanoviscometers: Applications for Measuring Cellular Physical Properties in 3D Cultures

SLAS TECHNOLOGY Translating Life Sciences Innovation ◽

10.1177/2472630319901262 ◽

2020 ◽

Vol 25 (3) ◽

pp. 234-246

Author(s):

Charles McRae White ◽

Mark A. Haidekker ◽

William S. Kisaalita

Keyword(s):

Cell Culture ◽

Cell Cultures ◽

Cell Biology ◽

Low Cost ◽

3D Cell Culture ◽

Biomechanical Properties ◽

Practical Applications ◽

3D Cell Cultures

New insights into the biomechanical properties of cells are revealing the importance of these properties and how they relate to underlying molecular, architectural, and behavioral changes associated with cell state and disease processes. However, the current understanding of how these in vitro biomechanical properties are associated with in vivo processes has been developed based on the traditional monolayer (two-dimensional [2D]) cell culture, which traditionally has not translated well to the three-dimensional (3D) cell culture and in vivo function. Many gold standard methods and tools used to observe the biomechanical properties of 2D cell cultures cannot be used with 3D cell cultures. Fluorescent molecules can respond to external factors almost instantaneously and require relatively low-cost instrumentation. In this review, we provide the background on fluorescent molecular rotors, which are attractive tools due to the relationship of their emission quantum yield with environmental microviscosity. We make the case for their use in both 2D and 3D cell cultures and speculate on their fundamental and practical applications in cell biology.

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Regulation of podocalyxin trafficking by Rab small GTPases in 2D and 3D epithelial cell cultures

The Journal of Cell Biology ◽

10.1083/jcb.201512024 ◽

2016 ◽

Vol 213 (3) ◽

pp. 355-369 ◽

Cited By ~ 48

Author(s):

Paulina S. Mrozowska ◽

Mitsunori Fukuda

Keyword(s):

Cell Cultures ◽

Three Dimensional ◽

Culture Conditions ◽

Small Gtpases ◽

Cell Polarization ◽

Rab Gtpases ◽

3D Cell Cultures ◽

Polarized Epithelia ◽

3D Environments ◽

2D And 3D

MDCK II cells, a widely used model of polarized epithelia, develop into different structures depending on culture conditions: two-dimensional (2D) monolayers when grown on synthetic supports or three-dimensional (3D) cysts when surrounded by an extracellular matrix. The establishment of epithelial polarity is accompanied by transcytosis of the apical marker podocalyxin from the outer plasma membrane to the newly formed apical domain, but its exact route and regulation remain poorly understood. Here, through comprehensive colocalization and knockdown screenings, we identified the Rab GTPases mediating podocalyxin transcytosis and showed that different sets of Rabs coordinate its transport during cell polarization in 2D and 3D structures. Moreover, we demonstrated that different Rab35 effectors regulate podocalyxin trafficking in 2D and 3D environments; trafficking is mediated by OCRL in 2D monolayers and ACAP2 in 3D cysts. Our results give substantial insight into regulation of the transcytosis of this apical marker and highlight differences between trafficking mechanisms in 2D and 3D cell cultures.

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