Integrated transcriptome and in vitro analysis revealed anti-proliferative effect of citral in human stomach cancer through apoptosis

This study is focused on antioxidant and anticancer capacity of bread enriched with broccoli sprouts (BS) in the light of their potential bioaccessibility and bioavailability. Generally, bread supplementation elevated antioxidant potential of product (both nonenzymatic and enzymatic antioxidant capacities); however, the increase was not correlated with the percent of BS. A replacement up to 2% of BS gives satisfactory overall consumers acceptability and desirable elevation of antioxidant potential. High activity was especially found for extracts obtained after simulated digestion, which allows assuming their protective effect for upper gastrointestinal tract; thus, the anticancer activity against human stomach cancer cells (AGS) was evaluated. A prominent cytostatic response paralleled by the inhibition of AGS motility in the presence of potentially mastication-extractable phytochemicals indicates that phenolic compounds of BS retain their biological activity in bread. Importantly, the efficient phenolics concentration was about 12 μM for buffer extract, 13 μM for extracts after digestionin vitro, and 7 μM for extract after absorptionin vitro. Our data confirm chemopreventive potential of bread enriched with BS and indicate that BS comprise valuable food supplement for stomach cancer chemoprevention.

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Generation of Human Stomach Cancer iPSC-Derived Organoids Induced by Helicobacter pylori Infection and Their Application to Gastric Cancer Research

Cells ◽

10.3390/cells11020184 ◽

2022 ◽

Vol 11 (2) ◽

pp. 184

Author(s):

Chia-Chen Ku ◽

Kenly Wuputra ◽

Jia-Bin Pan ◽

Chia-Pei Li ◽

Chung-Jung Liu ◽

...

Keyword(s):

Helicobacter Pylori ◽

Stem Cell ◽

Stomach Cancer ◽

Anticancer Agents ◽

Three Dimensional ◽

Human Stomach ◽

Human Stomach Cancer ◽

Stem Cell Niches

There is considerable cellular diversity in the human stomach, which has helped to clarify cell plasticity in normal development and tumorigenesis. Thus, the stomach is an interesting model for understanding cellular plasticity and for developing prospective anticancer therapeutic agents. However, many questions remain regarding the development of cancers in vivo and in vitro in two- or three-dimensional (2D/3D) cultures, as well as the role of Helicobacter pylori (H. p.) infection. Here, we focus on the characteristics of cancer stem cells and their derived 3D organoids in culture, including the formation of stem cell niches. We define the conditions required for such organoid culture in vitro and examine the ability of such models for testing the use of anticancer agents. We also summarize the signaling cascades and the specific markers of stomach-cancer-derived organoids induced by H. p. infection, and their stem cell niches.

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Cell cycle kinetics responses of human stomach cancer cells to reduction in polyamine levels by treatment with αDifluoromethylornithine in vitro

Investigational New Drugs ◽

10.1007/bf00173507 ◽

1986 ◽

Vol 4 (4) ◽

pp. 347-357 ◽

Cited By ~ 5

Author(s):

S. C. Barranco ◽

P. J. Ford ◽

C. M. Townsend

Keyword(s):

Cell Cycle ◽

Cancer Cells ◽

Stomach Cancer ◽

Human Stomach ◽

Cell Cycle Kinetics ◽

Human Stomach Cancer

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The inhibitory effect of amiloride on the growth of human stomach cancer cells in vitro

Gastroenterology ◽

10.1016/s0016-5085(00)84210-9 ◽

2000 ◽

Vol 118 (4) ◽

pp. A520

Author(s):

Jayoung Koo ◽

Duckkyung Kong ◽

Mooin Park ◽

Seonja Park ◽

Keunoh Chung ◽

...

Keyword(s):

Cancer Cells ◽

Stomach Cancer ◽

Inhibitory Effect ◽

Human Stomach ◽

Human Stomach Cancer

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The native structure of the eukaryotic ribosome

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100075890 ◽

1983 ◽

Vol 41 ◽

pp. 432-433

Author(s):

R.A. Milligan ◽

P.N.T. Unwin

Keyword(s):

Ribosomal Proteins ◽

Crystal Form ◽

Native Structure ◽

X Ray Diffraction ◽

Eukaryotic Ribosome ◽

Vitro Analysis ◽

In Vitro Analysis ◽

Resolution Structure ◽

Stable Unit

A detailed understanding of the mechanism of protein synthesis will ultimately depend on knowledge of the native structure of the ribosome. Towards this end we have investigated the low resolution structure of the eukaryotic ribosome embedded in frozen buffer, making use of a system in which the ribosomes crystallize naturally.The ribosomes in the cells of early chicken embryos form crystalline arrays when the embryos are cooled at 4°C. We have developed methods to isolate the stable unit of these arrays, the ribosome tetramer, and have determined conditions for the growth of two-dimensional crystals in vitro, Analysis of the proteins in the crystals by 2-D gel electrophoresis demonstrates the presence of all ribosomal proteins normally found in polysomes. There are in addition, four proteins which may facilitate crystallization. The crystals are built from two oppositely facing P4 layers and the predominant crystal form, accounting for >80% of the crystals, has the tetragonal space group P4212, X-ray diffraction of crystal pellets demonstrates that crystalline order extends to ~ 60Å.

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