scholarly journals Discovery of a new Theileria sp. closely related to Theileria annulata in cattle from Sri Lanka

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Thillaiampalam Sivakumar ◽  
Shiori Fujita ◽  
Bumduuren Tuvshintulga ◽  
Hemal Kothalawala ◽  
Seekkuge Susil Priyantha Silva ◽  
...  
Keyword(s):  
Sri Lanka ◽  
18S Rrna ◽  
Surface Protein ◽  
Theileria Annulata ◽  
Sri Lankan ◽  
Nucleotide Polymorphisms ◽  
Gene Sequences ◽  
Tropical Theileriosis ◽  
Single Nucleotide ◽  
Theileria Lestoquardi

Abstract Theileria annulata is a haemoprotozoan parasite that causes a cancer-like illness known as tropical theileriosis in cattle. In the course of analyzing the genetic diversity of T. annulata in Sri Lanka, we observed that merozoite-piroplasm surface antigen (tams1) and surface protein (tasp)-like gene sequences obtained from bovine blood DNA samples, which were PCR-positive for T. annulata, were conserved but shared low identity with T. annulata GenBank sequences. Moreover, the 18S rRNA sequences from the Sri Lankan samples contained ten unique single-nucleotide polymorphisms compared with all known T. annulata sequences. The cytochrome b (cob) gene sequences isolated from the Sri Lankan samples were highly conserved and shared low identity scores with similarly conserved T. annulata sequences from GenBank. Phylogenetic analysis showed that the Sri Lankan tams1-like, tasp-like, 18S rRNA, and cob sequences clustered together and formed sister clades to the common ancestors of all known T. annulata and Theileria lestoquardi sequences. These findings demonstrated that the Sri Lankan cattle were not infected with T. annulata but with a new Theileria sp. (designated as Theileria sp. Yokoyama) closely related to T. annulata.

Discrimination between Theileria lestoquardi and Theileria annulata in their vectors and hosts by RFLP based on the 18S rRNA gene

2004 ◽  
Vol 94 (4) ◽  
pp. 318-320 ◽  
Author(s):  
Eva Spitalska ◽  
Alessandra Torina ◽  
Vincenza Cannella ◽  
Santo Caracappa ◽  
Olivier A. E. Sparagano
Keyword(s):  
18S Rrna ◽  
18S Rrna Gene ◽  
Theileria Annulata ◽  
Rrna Gene ◽  
Theileria Lestoquardi

scholarly journals Island-wide diversity in single nucleotide polymorphisms of the Plasmodium vivax dihydrofolate reductase and dihydropteroate synthetase genes in Sri Lanka

2007 ◽  
Vol 6 (1) ◽  
pp. 28 ◽  
Author(s):  
Mette L Schousboe ◽  
Rupika S Rajakaruna ◽  
Ali Salanti ◽  
Hapuarachchige C Hapuarachchi ◽  
Gawrie NL Galappaththy ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Single Nucleotide Polymorphisms ◽  
Plasmodium Vivax ◽  
Dihydrofolate Reductase ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

scholarly journals Universal barcoding regions, rbcL, matK and trnH-psbA do not discriminate Cinnamomum species in Sri Lanka

PLoS ONE ◽  
2021 ◽  
Vol 16 (2) ◽  
pp. e0245592
Author(s):  
C. H. W. M. R. Bhagya Chandrasekara ◽  
D. Nathasha U. Naranpanawa ◽  
B. Supun Bandusekara ◽  
D. K. N. G. Pushpakumara ◽  
D. Siril A. Wijesundera ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Morphological Traits ◽  
Intraspecific Diversity ◽  
Sri Lankan ◽  
Single Nucleotide ◽  
Ceylon Cinnamon ◽  
Cultivated Species ◽  
Sustainable Conservation ◽  
Culinary Uses ◽  
Identification And Characterization

The genus Cinnamomum consists of about 250 species spread globally. Out of these, C. verum (C. zeylanicum), also known as true cinnamon or Ceylon cinnamon, has gained worldwide attention due to its culinary uses and medicinal values. Sri Lanka is the largest true cinnamon producer in the world and accounts for about 80–90% of global production. Other than the cultivated species, Sri Lankan natural vegetation is home to seven endemic wild species of the genus Cinnamomum. While these are underutilized, proper identification and characterization are essential steps in any sustainable conservation and utilization strategies. Currently, species identification is purely based on morphological traits, and intraspecific diversity has made it more challenging. In this study, all the eight Cinnamomum species found in Sri Lanka, C. capparu-coronde, C. citriodorum C. dubium, C. litseifolium, C. ovalifolium, C. rivulorum, C. sinharajaense, and C. verum were collected in triplicates and identified using typical morphological traits. DNA extracted with the same collection was assessed with universal barcoding regions, rbcL, matK, and trnH-psbA. While no intraspecific sequence differences were observed in C. citriodorum, C. rivulorum, and C. verum, the others had polymorphic sites in one, two, or all regions assessed. Interestingly, two individuals of C. sinharajaense had identical barcodes to the cultivated species C. verum, while the other one had one variable cite in matK region and three cites in trnH-psbA reigon. Further, one C. dubium and one C. capparu-coronde accession each had identical, rbcL, and trnH-psbA sequences while those had only a single nucleotide variation observed in matK region. Overall, the phylogeny of Cinnamomum species found in Sri Lanka could not be completely resolved with DNA barcoding regions studied.

M13.1 Association of single nucleotide polymorphisms in cytokine genes with preeclampsia in a Sinhalese population from Sri Lanka

2010 ◽  
Vol 1 ◽  
pp. S10
Author(s):  
Prabha Andraweera ◽  
Miss Rachael Nowak ◽  
Steven Thompson ◽  
Vajira Dissanayake ◽  
Rohan Jayasekara ◽  
...  
Keyword(s):  
Sri Lanka ◽  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Cytokine Genes
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