Publisher Correction: A cartridge based Point-of-Care device for complete blood count

This chapter reviews the causes and outlines an approach to the management of coagulopathy following cardiac surgery. Bleeding after cardiac surgery is common and expected up to a rate of 2 mL/kg/h for the first 6 hours. A more significant hemorrhage needs to be investigated and treated. Causes are often multifactorial. It is imperative that surgical causes be excluded early concomitant to providing resuscitation, investigating other medical causes for bleeding, and treating coagulopathy empirically until laboratory testing becomes available. The most frequent causes for coagulopathy post–cardiac surgery are excess heparinization, prolonged cardiopulmonary bypass time, hypothermia, acidosis, and preexisting bleeding diathesis. The management of coagulopathy implies maintenance of the normal physiological conditions for coagulation, reversal of excess heparinization, treatment of hyperfibrinolysis, maintaining normal levels of coagulation factors, and transfusion of platelets if thrombocytopenia or platelet dysfunction occurs. The chapter reviews what is involved in standard laboratory testing (complete blood count, prothrombin time, activated partial thromboplastin time, fibrinogen level, etc.) for coagulopathy. Also discussed is point-of-care testing and how the results from these tests should be interpreted. The chapter details the various blood products that are required in this scenario and suggests doses and transfusion thresholds.

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Determination of Hemoglobin Level Among 9-Month-Old Infants Visiting Well Child Clinic

Global Pediatric Health ◽

10.1177/2333794x211036629 ◽

2021 ◽

Vol 8 ◽

pp. 2333794X2110366

Author(s):

Pongtong Puranitee ◽

Sajee Fuangfu ◽

Oraporn Dumrongwongsiri

Keyword(s):

Infant Formula ◽

Associated Factors ◽

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Hemoglobin Level ◽

Breastfed Infants ◽

Confident Interval ◽

Prevalence Of Anemia

Screening for anemia is recommended among infants aged 9 to 12 months. This study was conducted to determine the prevalence of anemia among 9-month-old infants at Well Child Clinic, and associated factors with anemia. Well Child record of all visits during January to December 2018 were reviewed. Hemoglobin (Hb) was determined by complete blood count (CBC) or point-of-care Hb (POC-Hb). Anemia was found in 99 from 145 infants (68.3%). The prevalence of anemia was 33.3% and 72.8% when tested by CBC and POC-Hb, respectively. Breastfed Infants had significantly lower mean Hb than formula-fed infants. The odd ratio [95% confident interval] of having anemia among infants who were fed with infant formula were 0.37 [0.14-0.94]; P = .038 when compared to breastfed infants. There was a high proportion of anemia among 9-month-old infants in Rama-WCC and breastfeeding was associated with anemia in infants. The use of POC-Hb may overestimate the prevalence of anemia.

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Comparison between blood hemoglobin concentration determined by point-of-care device and complete blood count in adult patients with dengue

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009692 ◽

2021 ◽

Vol 15 (8) ◽

pp. e0009692

Author(s):

Kantasit Wisanuvej ◽

Kochawan Boonyawat ◽

Chutchaiwat Savetamornkul ◽

Sirapong Virapongsiri ◽

Jatupon Krongvorakul ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Hemoglobin Concentration ◽

Warning Signs ◽

Adult Patients ◽

Severe Dengue ◽

University Hospitals ◽

Automated Method ◽

Indispensable Tool

Background Hematocrit measurement has been an indispensable tool for monitoring plasma leakage and bleeding in dengue patients. However, hematocrit measurement by automated methods is hampered by frequent venipunctures. Utility of point-of-care hemoglobin (POC-Hb) test for monitoring dengue patients has not been established. We evaluated the relationship between hemoglobin measured by POC-Hb testing and hematocrit measured by the automated method in adult dengue patients. Methodology and principal findings Adult dengue patients were recruited at two university hospitals in Thailand from October 2019 to December 2020. POC-Hb test was performed using capillary blood simultaneously with venipuncture to obtain whole blood for an automated complete blood count (CBC) analysis. The correlation of hemoglobin and hematocrit measurement was evaluated. A total of 44 dengue patients were enrolled. Twenty-nine patients (65.9%) were female, with a median age of 31 years (interquartile range 22–41). Of the enrolled patients, 30 (68.2%), 11 (25.0%), and 3 (6.8%) were classified as dengue without warning signs, with warning signs, and severe dengue, respectively. Seven patients (15.9%) had hemoconcentration, and five patients (11.3%) had bleeding. A total of 216 pairs of POC-Hb and CBC were evaluated. A significant positive correlation was observed between hemoglobin measured by POC-Hb testing and hematocrit measured by an automated CBC (r = 0.869, p <0.001). Bland-Altman analysis between hemoglobin measured by POC-Hb testing and an automated CBC showed a bias of -0.43 (95% limit of agreement of -1.81 and 0.95). Using the cutoff of POC-Hb ≥20% as a criteria for hemoconcentration, the sensitivity and specificity of hemoconcentration detected by POC-Hb device were 71.4% and 100.0%, respectively. Conclusions Hemoglobin measurement by POC-Hb testing has a strong correlation with hematocrit in adult patients with dengue fever. However, the sensitivity in detecting hemoconcentration is fair. The adjunct use of capillary POC-Hb testing can decrease the frequency of venipuncture. Further study in children is encouraged.

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Evaluation of a new point of care automated complete blood count (CBC) analyzer in various clinical settings

Clinica Chimica Acta ◽

10.1016/j.cca.2007.12.006 ◽

2008 ◽

Vol 389 (1-2) ◽

pp. 120-125 ◽

Cited By ~ 29

Author(s):

L.V. Rao ◽

Björn A. Ekberg ◽

Diane Connor ◽

Felice Jakubiak ◽

Guy M. Vallaro ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Clinical Settings

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Complete Blood Count as point of care testing QBC STAR™: Preliminary evaluation

International Journal of Laboratory Hematology ◽

10.1111/ijlh.13515 ◽

2021 ◽

Author(s):

Gavino Napolitano ◽

Alessandra Caracciolo ◽

Sara Apassiti Esposito ◽

Nunzia Della Malva ◽

Barbara Manenti ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Preliminary Evaluation ◽

Point Of Care Testing

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ICSH Guideline for worldwide point-of-care testing in haematology with special reference to the complete blood count

International Journal of Laboratory Hematology ◽

10.1111/j.1751-553x.2008.01050.x ◽

2008 ◽

Vol 30 (2) ◽

pp. 105-116 ◽

Cited By ~ 34

Author(s):

C. BRIGGS ◽

J. CARTER ◽

S.-H. LEE ◽

L. SANDHAUS ◽

R. SIMON-LOPEZ ◽

...

Keyword(s):

Special Reference ◽

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Point Of Care Testing

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Complete blood count with differential: An effective diagnostic for IBS subtype in the context of BMI?

10.1101/608208 ◽

2019 ◽

Author(s):

JM Robinson ◽

CM Boulineaux ◽

KR Butler ◽

PV Joseph ◽

MT Murray ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Statistical Significance ◽

Poor Performance ◽

The United States ◽

Normal Weight ◽

Gi Symptoms ◽

Clinical Diagnoses ◽

Irritable Bowel

AbstractThe Complete Blood Count with Differential hematological assay is a mainstay diagnostic for point-of-care clinical diagnoses for a spectrum of diseases including infection, inflammation, anemia, and leukemia, and CBC-D profiles are under investigation as early prognostic biomarkers for leukemias and other diseases. Chronic abdominal pain (CAP) and irritable bowel syndrome (IBS) are prevalent gastrointestinal disorders in the United States, with obesity among the most common comorbidities. Often, IBS-like gastrointestinal (GI) symptoms persist after resolution of known inflammation and/or enteropathogenic infection, and current literature contains significant discussion of the extent to which IBS is within the biological spectrum of inflammatory disease. Obesity is also associated with generalized signatures of inflammation and may confound accurate diagnoses. We performed ANOVA, multiple means comparisons, statistical analyses of CBC data from our "Brain-Gut Natural History" (BGNH) clinical cohort, with additional ELISA assays for lipopolysaccharide binding protein (LBP), IL-10, cortisol, and ACTH, signatures of immune-inflammatory response and Hypothalamic-Pituitary-Adrenal (HPA) axis activity, respectively. BGNH cohort includes healthy and overweight individuals diagnosed with IBS diarrhea-(IBS-D) and constipation-predominant (IBS-C) subtypes. We identified several potentially significant markers for IBS-D and IBS-C, notably IL-10, mean platelet volume (MPV), with LBP and monocyte percent also showing some statistical significance. Weight also showed significant results for overweight vs. normal weight, regardless of IBS subtype, particularly for Cortisol. CBC-D predictive profiles for IBS subtype and weight were identified using discriminant functions analysis and show that predictivity of marker profiles have poor performance relative to their normal weight subsets. Further refinement of this analysis will be performed utilizing increased sample size, additional molecular profiles, and enhanced statistical analysis.

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A cartridge based Point-of-Care device for complete blood count

Scientific Reports ◽

10.1038/s41598-019-54006-3 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Usama Abbasi ◽

Prasanta Chowdhury ◽

Sasikala Subramaniam ◽

Prakhar Jain ◽

Nitin Muthe ◽

...

Keyword(s):

Credit Card ◽

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Rapid Diagnostics ◽

Lab On Chip ◽

Skilled Manpower ◽

Chip Fabrication ◽

On Chip ◽

The Cost

AbstractWe demonstrate a proprietary lab-on-chip/μ TAS technology platform for a regulatory grade portable instrument for complete blood count (CBC) hematology tests including 3 part differential WBCs, RBCs, platelet and hemoglobin for rapid diagnostics at the point of care in resource-poor settings. Presently, diagnostics based on blood tests are confined to centralized laboratory settings, dependent on large footprint and expensive cytometers or on a microscope, requiring trained laboratory technicians. Consequently, such facilities are not present in rural and semi-urban settings, where there are opportunities and challenges in delivering efficient healthcare infrastructure at an affordable cost in resource-challenged environments. Our proposed design leverages advances in microfluidics and lab-on-chip fabrication techniques to miniaturize the conventional cytometer and bring down the cost significantly. The device can be operated autonomously, without skilled manpower, by primary healthcare professionals in the field and by patients (like glucose self-test devices). The instrument consists of a single-use chip, the size of a credit card, pre-loaded with reagents, in which the sample is loaded, and which is fluidically insulated from the environment. The controller, the size of a toaster, performs the necessary fluid handling and the impedance measurements to deliver the results in minutes.

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An Artificial Intelligence-Assisted Diagnostic Platform for Rapid Near-Patient Hematology

10.1101/2021.04.27.21255770 ◽

2021 ◽

Author(s):

Neta Bachar ◽

Dana Benbassat ◽

David Brailovsky ◽

Yochay Eshel ◽

Dan Glück ◽

...

Keyword(s):

Artificial Intelligence ◽

Blood Count ◽

Clinical Laboratory ◽

Complete Blood Count ◽

Point Of Care ◽

Finger Prick ◽

Repeatability And Reproducibility ◽

Current Point ◽

High Repeatability ◽

Hematology Analyzers

Hematology analyzers capable of performing complete blood count (CBC) have lagged in their prevalence at the point-of-care. Sight OLO® (Sight Diagnostics, Israel) is a novel hematological platform which provides a 19 parameter, five-part differential CBC, and is designed to address the limitations in current point-of-care hematology analyzers using recent advances in artificial intelligence (AI) and computer vision. Accuracy, repeatability, and flagging capabilities of OLO were compared with the Sysmex XN-Series System (Sysmex, Japan). Matrix studies compared performance using venous, capillary and direct-from-finger-prick blood samples. Regression analysis shows strong concordance between OLO and the Sysmex XN, demonstrating that OLO performs with high accuracy for all CBC parameters. High repeatability and reproducibility were demonstrated for most of the testing parameters. The analytical performance of the OLO hematology analyzer was validated in a multicenter clinical laboratory setting, demonstrating its accuracy and comparability to clinical laboratory-based hematology analyzers. Furthermore, the study demonstrated the validity of CBC analysis of samples collected directly from fingerpricks.One Sentence SummaryWe present a novel diagnostic platform based on artificial intelligence-assisted image analysis that is capable of performing rapid complete blood count from venous, capillary, and finger-prick samples in near-patient settings.

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