Complete blood count with differential: An effective diagnostic for IBS subtype in the context of BMI?

AbstractThe Complete Blood Count with Differential hematological assay is a mainstay diagnostic for point-of-care clinical diagnoses for a spectrum of diseases including infection, inflammation, anemia, and leukemia, and CBC-D profiles are under investigation as early prognostic biomarkers for leukemias and other diseases. Chronic abdominal pain (CAP) and irritable bowel syndrome (IBS) are prevalent gastrointestinal disorders in the United States, with obesity among the most common comorbidities. Often, IBS-like gastrointestinal (GI) symptoms persist after resolution of known inflammation and/or enteropathogenic infection, and current literature contains significant discussion of the extent to which IBS is within the biological spectrum of inflammatory disease. Obesity is also associated with generalized signatures of inflammation and may confound accurate diagnoses. We performed ANOVA, multiple means comparisons, statistical analyses of CBC data from our "Brain-Gut Natural History" (BGNH) clinical cohort, with additional ELISA assays for lipopolysaccharide binding protein (LBP), IL-10, cortisol, and ACTH, signatures of immune-inflammatory response and Hypothalamic-Pituitary-Adrenal (HPA) axis activity, respectively. BGNH cohort includes healthy and overweight individuals diagnosed with IBS diarrhea-(IBS-D) and constipation-predominant (IBS-C) subtypes. We identified several potentially significant markers for IBS-D and IBS-C, notably IL-10, mean platelet volume (MPV), with LBP and monocyte percent also showing some statistical significance. Weight also showed significant results for overweight vs. normal weight, regardless of IBS subtype, particularly for Cortisol. CBC-D predictive profiles for IBS subtype and weight were identified using discriminant functions analysis and show that predictivity of marker profiles have poor performance relative to their normal weight subsets. Further refinement of this analysis will be performed utilizing increased sample size, additional molecular profiles, and enhanced statistical analysis.

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Haematological reference of snakes: Amazon tree boa (Corallus hortulanus, Linnaeus, 1758) and Burmenese Python (Python bivittatus, Kuhl, 1820) in captive

Arquivo Brasileiro de Medicina Veterinária e Zootecnia ◽

10.1590/1678-4162-9865 ◽

2018 ◽

Vol 70 (4) ◽

pp. 1172-1178

Author(s):

A.E. Quadrini ◽

V.C. Garcia ◽

B.C. Freire ◽

M.F.M. Martins

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Leukocyte Count ◽

Differential Leukocyte Count ◽

Healthy State ◽

The Family ◽

In Captivity ◽

Clinical Diagnoses ◽

Maintenance Techniques ◽

Python Bivittatus

ABSTRACT Hematology has become important for making clinical diagnoses in snakes because maintenance techniques in captivity have been improving and increasing their life expectancy. The emergence of diseases in captivity requires the creation of parameters to understand the species in their healthy state. The possible association between the onset of neoplasia, immunosuppression, and viral infection are also important factors to consider. Thus, hematology is a fundamental tool for observing the responses of animals to diseases and treatments. The present study aims to study hematology between two species of snakes and compare the profiles observed with published results. Blood samples were collected from 16 male and female snakes: seven Corallus hortulanus from the family Boidae and nine Python bivittatus from the family Pythonidae belonging to the Biological Museum of the Butantan Institute, São Paulo, Brazil. Complete blood count results including blood smear, total erythrocyte count, leukocytes, and differential leukocyte count were evaluated. The data obtained in this study could help with the diagnosis, the treatment of snakes in captive conditions and in of nature conservation programs.

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332: Can a point-of-care hemoglobin device replace complete blood count for fetal blood sampling during intrauterine transfusion?

American Journal of Obstetrics and Gynecology ◽

10.1016/j.ajog.2017.10.268 ◽

2018 ◽

Vol 218 (1) ◽

pp. S207-S208

Author(s):

Cathy Monteith ◽

Jahan Jadauji ◽

Hala Abu ◽

Ann M. McHugh ◽

Jennifer C. Donnelly ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Blood Sampling ◽

Fetal Blood ◽

Fetal Blood Sampling ◽

Intrauterine Transfusion

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Coagulopathy in Cardiac Surgery: Etiology and Treatment Options

Cardiothoracic Critical Care ◽

10.1093/med/9780190082482.003.0033 ◽

2020 ◽

pp. 313-322

Author(s):

Dana Teodorescu ◽

Caroline Larkin

Keyword(s):

Cardiac Surgery ◽

Laboratory Testing ◽

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Treatment Options ◽

Coagulation Factors ◽

Bleeding Diathesis ◽

Standard Laboratory ◽

Post Cardiac Surgery

This chapter reviews the causes and outlines an approach to the management of coagulopathy following cardiac surgery. Bleeding after cardiac surgery is common and expected up to a rate of 2 mL/kg/h for the first 6 hours. A more significant hemorrhage needs to be investigated and treated. Causes are often multifactorial. It is imperative that surgical causes be excluded early concomitant to providing resuscitation, investigating other medical causes for bleeding, and treating coagulopathy empirically until laboratory testing becomes available. The most frequent causes for coagulopathy post–cardiac surgery are excess heparinization, prolonged cardiopulmonary bypass time, hypothermia, acidosis, and preexisting bleeding diathesis. The management of coagulopathy implies maintenance of the normal physiological conditions for coagulation, reversal of excess heparinization, treatment of hyperfibrinolysis, maintaining normal levels of coagulation factors, and transfusion of platelets if thrombocytopenia or platelet dysfunction occurs. The chapter reviews what is involved in standard laboratory testing (complete blood count, prothrombin time, activated partial thromboplastin time, fibrinogen level, etc.) for coagulopathy. Also discussed is point-of-care testing and how the results from these tests should be interpreted. The chapter details the various blood products that are required in this scenario and suggests doses and transfusion thresholds.

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Determination of Hemoglobin Level Among 9-Month-Old Infants Visiting Well Child Clinic

Global Pediatric Health ◽

10.1177/2333794x211036629 ◽

2021 ◽

Vol 8 ◽

pp. 2333794X2110366

Author(s):

Pongtong Puranitee ◽

Sajee Fuangfu ◽

Oraporn Dumrongwongsiri

Keyword(s):

Infant Formula ◽

Associated Factors ◽

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Hemoglobin Level ◽

Breastfed Infants ◽

Confident Interval ◽

Prevalence Of Anemia

Screening for anemia is recommended among infants aged 9 to 12 months. This study was conducted to determine the prevalence of anemia among 9-month-old infants at Well Child Clinic, and associated factors with anemia. Well Child record of all visits during January to December 2018 were reviewed. Hemoglobin (Hb) was determined by complete blood count (CBC) or point-of-care Hb (POC-Hb). Anemia was found in 99 from 145 infants (68.3%). The prevalence of anemia was 33.3% and 72.8% when tested by CBC and POC-Hb, respectively. Breastfed Infants had significantly lower mean Hb than formula-fed infants. The odd ratio [95% confident interval] of having anemia among infants who were fed with infant formula were 0.37 [0.14-0.94]; P = .038 when compared to breastfed infants. There was a high proportion of anemia among 9-month-old infants in Rama-WCC and breastfeeding was associated with anemia in infants. The use of POC-Hb may overestimate the prevalence of anemia.

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Comparison between blood hemoglobin concentration determined by point-of-care device and complete blood count in adult patients with dengue

PLoS Neglected Tropical Diseases ◽

10.1371/journal.pntd.0009692 ◽

2021 ◽

Vol 15 (8) ◽

pp. e0009692

Author(s):

Kantasit Wisanuvej ◽

Kochawan Boonyawat ◽

Chutchaiwat Savetamornkul ◽

Sirapong Virapongsiri ◽

Jatupon Krongvorakul ◽

...

Keyword(s):

Blood Count ◽

Complete Blood Count ◽

Point Of Care ◽

Hemoglobin Concentration ◽

Warning Signs ◽

Adult Patients ◽

Severe Dengue ◽

University Hospitals ◽

Automated Method ◽

Indispensable Tool

Background Hematocrit measurement has been an indispensable tool for monitoring plasma leakage and bleeding in dengue patients. However, hematocrit measurement by automated methods is hampered by frequent venipunctures. Utility of point-of-care hemoglobin (POC-Hb) test for monitoring dengue patients has not been established. We evaluated the relationship between hemoglobin measured by POC-Hb testing and hematocrit measured by the automated method in adult dengue patients. Methodology and principal findings Adult dengue patients were recruited at two university hospitals in Thailand from October 2019 to December 2020. POC-Hb test was performed using capillary blood simultaneously with venipuncture to obtain whole blood for an automated complete blood count (CBC) analysis. The correlation of hemoglobin and hematocrit measurement was evaluated. A total of 44 dengue patients were enrolled. Twenty-nine patients (65.9%) were female, with a median age of 31 years (interquartile range 22–41). Of the enrolled patients, 30 (68.2%), 11 (25.0%), and 3 (6.8%) were classified as dengue without warning signs, with warning signs, and severe dengue, respectively. Seven patients (15.9%) had hemoconcentration, and five patients (11.3%) had bleeding. A total of 216 pairs of POC-Hb and CBC were evaluated. A significant positive correlation was observed between hemoglobin measured by POC-Hb testing and hematocrit measured by an automated CBC (r = 0.869, p <0.001). Bland-Altman analysis between hemoglobin measured by POC-Hb testing and an automated CBC showed a bias of -0.43 (95% limit of agreement of -1.81 and 0.95). Using the cutoff of POC-Hb ≥20% as a criteria for hemoconcentration, the sensitivity and specificity of hemoconcentration detected by POC-Hb device were 71.4% and 100.0%, respectively. Conclusions Hemoglobin measurement by POC-Hb testing has a strong correlation with hematocrit in adult patients with dengue fever. However, the sensitivity in detecting hemoconcentration is fair. The adjunct use of capillary POC-Hb testing can decrease the frequency of venipuncture. Further study in children is encouraged.

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Diagnostic Testing and Antibiotic Use in Young Children With Community-Acquired Pneumonia in the United States, 2008–2015

Journal of the Pediatric Infectious Diseases Society ◽

10.1093/jpids/piz026 ◽

2019 ◽

Vol 9 (2) ◽

pp. 248-252 ◽

Cited By ~ 3

Author(s):

Todd A Florin ◽

Terri Byczkowski ◽

Jeffrey S Gerber ◽

Richard Ruddy ◽

Nathan Kuppermann

Keyword(s):

United States ◽

Young Children ◽

Blood Count ◽

Complete Blood Count ◽

Diagnostic Testing ◽

Antibiotic Use ◽

The United States ◽

National Sample ◽

Community Acquired Pneumonia ◽

Over Time

Abstract Diagnostic testing and antibiotics are not routinely recommended for young children with community-acquired pneumonia. In a national sample of >6 million outpatient 1- to 6-year-olds with community-acquired pneumonia between 2008 and 2015, a complete blood count was obtained for 8.6% (95% confidence interval [CI], 6.1%–11.1%), radiography was performed for 43% (95% CI, 36%–50%), and antibiotics were given for 73.9% (95% CI, 67.1%–80.7%). There were no changes in testing or antibiotic use over time.

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