scholarly journals Improving skeleton algorithm for helping Caenorhabditis elegans trackers

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Pablo E. Layana Castro ◽  
Joan Carles Puchalt ◽  
Antonio-José Sánchez-Salmerón

AbstractOne of the main problems when monitoring Caenorhabditis elegans nematodes (C. elegans) is tracking their poses by automatic computer vision systems. This is a challenge given the marked flexibility that their bodies present and the different poses that can be performed during their behaviour individually, which become even more complicated when worms aggregate with others while moving. This work proposes a simple solution by combining some computer vision techniques to help to determine certain worm poses and to identify each one during aggregation or in coiled shapes. This new method is based on the distance transformation function to obtain better worm skeletons. Experiments were performed with 205 plates, each with 10, 15, 30, 60 or 100 worms, which totals 100,000 worm poses approximately. A comparison of the proposed method was made to a classic skeletonisation method to find that 2196 problematic poses had improved by between 22% and 1% on average in the pose predictions of each worm.

Metrologiya ◽  
2020 ◽  
pp. 15-37
Author(s):  
L. P. Bass ◽  
Yu. A. Plastinin ◽  
I. Yu. Skryabysheva

Use of the technical (computer) vision systems for Earth remote sensing is considered. An overview of software and hardware used in computer vision systems for processing satellite images is submitted. Algorithmic methods of the data processing with use of the trained neural network are described. Examples of the algorithmic processing of satellite images by means of artificial convolution neural networks are given. Ways of accuracy increase of satellite images recognition are defined. Practical applications of convolution neural networks onboard microsatellites for Earth remote sensing are presented.


2017 ◽  
Vol 2 (1) ◽  
pp. 80-87
Author(s):  
Puyda V. ◽  
◽  
Stoian. A.

Detecting objects in a video stream is a typical problem in modern computer vision systems that are used in multiple areas. Object detection can be done on both static images and on frames of a video stream. Essentially, object detection means finding color and intensity non-uniformities which can be treated as physical objects. Beside that, the operations of finding coordinates, size and other characteristics of these non-uniformities that can be used to solve other computer vision related problems like object identification can be executed. In this paper, we study three algorithms which can be used to detect objects of different nature and are based on different approaches: detection of color non-uniformities, frame difference and feature detection. As the input data, we use a video stream which is obtained from a video camera or from an mp4 video file. Simulations and testing of the algoritms were done on a universal computer based on an open-source hardware, built on the Broadcom BCM2711, quad-core Cortex-A72 (ARM v8) 64-bit SoC processor with frequency 1,5GHz. The software was created in Visual Studio 2019 using OpenCV 4 on Windows 10 and on a universal computer operated under Linux (Raspbian Buster OS) for an open-source hardware. In the paper, the methods under consideration are compared. The results of the paper can be used in research and development of modern computer vision systems used for different purposes. Keywords: object detection, feature points, keypoints, ORB detector, computer vision, motion detection, HSV model color


2021 ◽  
Vol 14 (3) ◽  
pp. 1-17
Author(s):  
Elena Villaespesa ◽  
Seth Crider

Computer vision algorithms are increasingly being applied to museum collections to identify patterns, colors, and subjects by generating tags for each object image. There are multiple off-the-shelf systems that offer an accessible and rapid way to undertake this process. Based on the highlights of the Metropolitan Museum of Art's collection, this article examines the similarities and differences between the tags generated by three well-known computer vision systems (Google Cloud Vision, Amazon Rekognition, and IBM Watson). The results provide insights into the characteristics of these taxonomies in terms of the volume of tags generated for each object, their diversity, typology, and accuracy. In consequence, this article discusses the need for museums to define their own subject tagging strategy and selection criteria of computer vision tools based on their type of collection and tags needed to complement their metadata.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Joan Carles Puchalt ◽  
Antonio-José Sánchez-Salmerón ◽  
Eugenio Ivorra ◽  
Silvia Llopis ◽  
Roberto Martínez ◽  
...  

AbstractTraditionally Caenorhabditis elegans lifespan assays are performed by manually inspecting nematodes with a dissection microscope, which involves daily counting of live/dead worms cultured in Petri plates for 21–25 days. This manual inspection requires the screening of hundreds of worms to ensure statistical robustness, and is therefore a time-consuming approach. In recent years, various automated artificial vision systems have been reported to increase the throughput, however they usually provide less accurate results than manual assays. The main problems identified when using these vision systems are the false positives and false negatives, which occur due to culture media changes, occluded zones, dirtiness or condensation of the Petri plates. In this work, we developed and described a new C. elegans monitoring machine, SiViS, which consists of a flexible and compact platform design to analyse C. elegans cultures using the standard Petri plates seeded with E. coli. Our system uses an active vision illumination technique and different image-processing pipelines for motion detection, both previously reported, providing a fully automated image processing pipeline. In addition, this study validated both these methods and the feasibility of the SiViS machine for lifespan experiments by comparing them with manual lifespan assays. Results demonstrated that the automated system yields consistent replicates (p-value log rank test 0.699), and there are no significant differences between automated system assays and traditionally manual assays (p-value 0.637). Finally, although we have focused on the use of SiViS in longevity assays, the system configuration is flexible and can, thus, be adapted to other C. elegans studies such as toxicity, mobility and behaviour.


2018 ◽  
Vol 224 ◽  
pp. 01088 ◽  
Author(s):  
Yaroslav Kulkov ◽  
Arkady Zhiznyakov ◽  
Denis Privezentsev

The aim is an experimental research on the flat objects recognition using dimensionless marks of the contours of their binary images and determining the possibility of applying this method in computer vision systems of assembly robots. The main problem with the automation of assembly operations is the recognition of parts for the subsequent picking up of the robot arm. The basis for the formation of attribute vectors is the characteristics of the image contour. Recognition of a class of an unknown object consists in receipt of its contour, calculation of primary parameters and forming of a vector of dimensionless marks. Further mean square deviations of its vector of dimensionless marks from all reference are calculated. The minimum value of a deviation will specify probable belonging to the corresponding class.


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