scholarly journals The epicardial delivery of cardiosphere derived cells or their extracellular vesicles is safe but of limited value in experimental infarction

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Verónica Crisóstomo ◽  
Claudia Baéz-Diaz ◽  
Virginia Blanco-Blázquez ◽  
Verónica Álvarez ◽  
Esther López-Nieto ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Cardiac Function ◽  
Extracellular Vesicles ◽  
Experimental Myocardial Infarction ◽  
Pathological Examination ◽  
Resonance Imaging ◽  
Coronary Embolization ◽  
Pericardial Adhesions ◽  
Improved Function ◽  
Mini Thoracotomy

AbstractThe epicardial administration of therapeutics via the pericardial sac offers an attractive route, since it is minimally invasive and carries no risks of coronary embolization. The aim of this study was to assess viability, safety and effectiveness of cardiosphere-derived cells (CDCs), their extracellular vesicles (EVs) or placebo administered via a mini-thoracotomy 72 h after experimental infarction in swine. The epicardial administration was completed successfully in all cases in a surgery time (knife-to-skin) below 30 min. No significant differences between groups were found in cardiac function parameters evaluated using magnetic resonance imaging before therapy and at the end of the study, despite a trend towards improved function in CDC-treated animals. Moreover, infarct size at 10 weeks was smaller in treated animals, albeit not significantly. Arrhythmia inducibility did not differ between groups. Pathological examination showed no differences, nor were there any pericardial adhesions evidenced in any case 10 weeks after surgery. These results show that the epicardial delivery of CDCs or their EVs is safe and technically easy 3 days after experimental myocardial infarction in swine, but it does not appear to have any beneficial effect on cardiac function. Our results do not support clinical translation of these therapies as implemented in this work.

scholarly journals Epicardial Delivery of Cardiosphere Derived Cells or Their Extracellular Vesicles in a Relevant Infarcted Swine Model: Safe, Easy but of Limited Effectiveness.

2021 ◽  
Author(s):  
Verónica Crisostomo ◽  
Claudia Baez-Diaz ◽  
Virginia Blanco-Blazquez ◽  
Verónica Alvarez ◽  
Esther Lopez-Nieto ◽  
...  
Keyword(s):  
Cardiac Function ◽  
Extracellular Vesicles ◽  
Experimental Myocardial Infarction ◽  
Pathological Examination ◽  
Swine Model ◽  
Resonance Imaging ◽  
Limited Effectiveness ◽  
Coronary Embolization ◽  
Pericardial Adhesions ◽  
Improved Function

Abstract The epicardial administration of therapeutics via the pericardial sac offers an attractive route, since it is minimally invasive and carries no risks of coronary embolization. The aim of this study was to assess viability, safety and effectiveness of Cardiosphere-Derived Cells (CDCs), their extracellular vesicles (EVs) or placebo administered via a mini-thoracotomy 72h after experimental infarction in swine. The epicardial administration was completed successfully in all cases in a surgery time (knife-to-skin) below 30 minutes. No significant differences between groups were found in cardiac function parameters evaluated using magnetic resonance imaging before therapy and at the end of the study, despite a trend towards improved function in CDC-treated animals. Moreover, infarct size at 10 weeks was smaller in treated animals, albeit not significantly. Arrhythmia inducibility did not differ between groups. Pathological examination showed no differences, nor were there any pericardial adhesions evidenced in any case 10 weeks after surgery. These results show that the epicardial delivery of CDCs or their EVs is safe and technically easy 3 days after experimental myocardial infarction in swine, but it does not appear to have any beneficial effect on cardiac function. Our results do not support clinical translation of these therapies as implemented in this work.

scholarly journals Laparoscopic resection of two peritoneal loose bodies on the rectosigmoid colon

2018 ◽  
Vol 7 (2) ◽  
pp. 736
Author(s):  
Ken-ichi Honda ◽  
Tomoko Nakagawa ◽  
Yasushi Kurihara ◽  
Koji Kajitani ◽  
Tetsuji Ando ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Laparoscopic Resection ◽  
Preoperative Magnetic Resonance Imaging ◽  
Pathological Examination ◽  
Resonance Imaging ◽  
Loose Bodies ◽  
Rectosigmoid Colon ◽  
Preoperative Magnetic Resonance

Laparoscopic examination of a 77-year-old woman revealed two peritoneal loose bodies connected to fatty appendices on the rectosigmoid colon and resected at the stalks. The peritoneal loose bodies were found to be fat-containing masses on preoperative magnetic resonance imaging, and postoperative pathological examination revealed fat degeneration tissue with or without fibrous outer layers.

scholarly journals Feasibility and reproducibility of biventricular volumetric assessment of cardiac function during exercise using real-time radialk-tSENSE magnetic resonance imaging

2009 ◽  
Vol 29 (5) ◽  
pp. 1062-1070 ◽  
Author(s):  
Philipp Lurz ◽  
Vivek Muthurangu ◽  
Silvia Schievano ◽  
Johannes Nordmeyer ◽  
Philipp Bonhoeffer ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Cardiac Function ◽  
Real Time ◽  
Resonance Imaging ◽  
Volumetric Assessment

scholarly journals Anterior cruciate ligament ganglion: case report

2002 ◽  
Vol 120 (6) ◽  
pp. 195-197 ◽  
Author(s):  
André Pedrinelli ◽  
Fábio Bonini Castellana ◽  
Ricardo Bragança de Vasconcellos Fontes ◽  
Rafael Ferreira Coelho ◽  
Luiz Álvaro de Menezes F°.
Keyword(s):  
Magnetic Resonance Imaging ◽  
Anterior Cruciate Ligament ◽  
Case Report ◽  
Magnetic Resonance ◽  
Cruciate Ligament ◽  
Clinical Signs ◽  
Left Knee ◽  
Pathological Examination ◽  
Resonance Imaging ◽  
Anterior Cruciate

CONTEXT: A ganglion is a cystic formation close to joints or tendinous sheaths, frequently found in the wrist, foot or knee. Intra-articular ganglia of the knee are rare, and most of them are located in the anterior cruciate ligament. The clinical picture for these ganglia comprises pain and movement restrictions in the knee, causing significant impairment to the patient. Symptoms are non-specific, and anterior cruciate ligament ganglia are usually diagnosed through magnetic resonance imaging or arthroscopy. Not all ganglia diagnosed through magnetic resonance imaging need to undergo surgical treatment: only those that cause clinical signs and symptoms do. Surgical results are considered good or excellent in the vast majority of cases. CASE REPORT: A 29-year-old male presented with pain in the left knee during a marathon race. Physical examination revealed limitation in the maximum range of knee extension and pain in the posterior aspect of the left knee. Radiographs of the left knee were normal, but magnetic resonance imaging revealed a multi-lobed cystic structure adjacent to the anterior cruciate ligament, which resembled a ganglion cyst. The mass was removed through arthroscopy, and pathological examination revealed a synovial cyst. Patient recovery was excellent, and he resumed his usual training routine five months later.

scholarly journals Assessment of cardiac function in three mouse dystrophinopathies by magnetic resonance imaging

2012 ◽  
Vol 22 (5) ◽  
pp. 418-426 ◽  
Author(s):  
Ingrid E.C. Verhaart ◽  
Rianne J.M. van Duijn ◽  
Brigit den Adel ◽  
Arno A.W. Roest ◽  
Jan J.G.M. Verschuuren ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Cardiac Function ◽  
Resonance Imaging

Spinal intramedullary ependymal cyst and tethered cord in an adult

2006 ◽  
Vol 4 (6) ◽  
pp. 506-508 ◽  
Author(s):  
Erhan Takci ◽  
Goksin Sengul ◽  
Muzaffer Keles
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Tethered Cord ◽  
Pathological Examination ◽  
Resonance Imaging ◽  
Ependymal Cyst ◽  
Congenital Lesion ◽  
Post Surgery

✓ Spinal intramedullary ependymal cysts are extremely rare. Fourteen cases have been reported in the literature, and only one was associated with another congenital lesion. The authors describe the case of a 33-year-old man in whom an intramedullary ependymal cyst and filar lipoma were present. These lesions were diagnosed using magnetic resonance imaging and were treated surgically. Pathological examination confirmed the diagnosis. At 6 months post-surgery, there was no evidence of recurrence.

Strategic reconstruction of macrophage-derived extracellular vesicles as a magnetic resonance imaging contrast agent

2020 ◽  
Vol 8 (10) ◽  
pp. 2887-2904 ◽  
Author(s):  
Sagar Rayamajhi ◽  
Ramesh Marasini ◽  
Tuyen Duong Thanh Nguyen ◽  
Brandon L. Plattner ◽  
David Biller ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Contrast Agent ◽  
Extracellular Vesicles ◽  
Resonance Imaging ◽  
Clinical Imaging ◽  
Clinical Investigations ◽  
Magnetic Resonance Imaging Contrast ◽  
Downstream Analysis ◽  
Imaging Contrast Agent

Reconstruction of extracellular vesicles with imaging agents allows precise downstream analysis using clinical imaging modalities, for example, MRI. This will further improve the biocompatibility of agents thereby enhancing clinical investigations.

scholarly journals Relationship Between Magnetic Resonance T2-Mapping and Matrix Metalloproteinase 1,3 in Knee Osteoarthritis

2020 ◽  
Author(s):  
Lei Shi ◽  
Kexin Wang ◽  
Jinghong Yu ◽  
Mingkai Li ◽  
Guangmei Men ◽  
...  
Keyword(s):  
Magnetic Resonance Imaging ◽  
Magnetic Resonance ◽  
Matrix Metalloproteinase ◽  
Knee Osteoarthritis ◽  
T2 Mapping ◽  
Cartilage Degeneration ◽  
Pathological Examination ◽  
Lesion Group ◽  
Control Group ◽  
Resonance Imaging

Abstract Objective To investigate the relationship between quantitative analysis of MRI (T2-mapping) and the expression of matrix metalloproteinase (MMP-1, MMP-3) in osteoarthritis of the knee joint and the role of MMP-1,3 in the pathogenesis of osteoarthritis. Methods Thirty cases of knee osteoarthritis (KOA) patients with total knee arthroplasty (TKA) (lesion group) and 30 healthy adult volunteers (control group) were scanned with 1.5 T routine MR and T2-mapping, and their T2 values were measured and statistically analyzed. The pathological examination of the knee cartilage that was replaced during the operation and the immunohistochemical assay were used to measure the expression of MMP-1,3. The correlation between the T2 value of magnetic resonance imaging and the expression of MMP-1,3 was analyzed. Results (1) According to the Recht grading standard for magnetic resonance, the T2 value of magnetic resonance increased significantly with the increase of cartilage degeneration. The differences in T2 values between each level and the normal group were statistically significant (P  < 0.05). (2) The T2 value of magnetic resonance imaging increased with the severity of the cartilage degeneration pathological Mankin grading, and the difference was statistically significant (P  <  0.05). (3) The expression of MMP-1,3 increased with cartilage degeneration. (4) The T2 value and the expression of MMP-1 in cartilage showed a linear trend. The result of Spearman correlation analysis showed that the expression of MMP-1,3 increased as the cartilage T2 value increased. There was a positive linear correlation between the two. Conclusion The T2 value of magnetic resonance increased with the degeneration of KOA cartilage. The expression of MMP-1,3 increased with the severity of articular cartilage destruction. The T2 value of KOA magnetic resonance was positively correlated with the expression of MMP-1,3.

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