Optimization of critical parameters for coating of polymeric nanoparticles with plasma membrane vesicles by sonication

AbstractTop-down functionalization of nanoparticles with cellular membranes imparts nanoparticles with enhanced bio-interfacing capabilities. Initial methods for membrane coating involved physical co-extrusion of nanoparticles and membrane vesicles through a porous membrane; however, recent works employ sonication as the disruptive force to reform membranes around the surface of nanoparticles. Although sonication is widely used, there remains a paucity of information on the effects of sonication variables on coating efficiency, leading to inconsistent membrane coating across studies. In this work, we present a systematic analysis of the sonication parameters that influence the membrane coating. The results showed that sonication amplitude, time, temperature, membrane ratio, sample volume, and density need to be considered in order to optimize membrane coating of polymeric nanoparticles.

Download Full-text

Characterization of a glutamine/proton cotransporter from Ricinus communis roots using isolated plasma membrane vesicles

Physiologia Plantarum ◽

10.1034/j.1399-3054.1994.910411.x ◽

1994 ◽

Vol 91 (4) ◽

pp. 623-630

Author(s):

Kim Weston ◽

J. L. Hall ◽

Lorraine E. Williams

Keyword(s):

Plasma Membrane ◽

Ricinus Communis ◽

Membrane Vesicles ◽

Plasma Membrane Vesicles

Download Full-text

Faculty Opinions recommendation of In vitro fusion between Saccharomyces cerevisiae secretory vesicles and cytoplasmic-side-out plasma membrane vesicles.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1010821.167908 ◽

2002 ◽

Author(s):

Miguel Penalva

Keyword(s):

Saccharomyces Cerevisiae ◽

Plasma Membrane ◽

Membrane Vesicles ◽

Secretory Vesicles ◽

Plasma Membrane Vesicles ◽

Cytoplasmic Side

Download Full-text

Ca2+-ATPase-driven calcium accumulation in Ustilago maydis plasma membrane vesicles

Microbiology ◽

10.1099/13500872-140-11-3047 ◽

1994 ◽

Vol 140 (11) ◽

pp. 3047-3051 ◽

Cited By ~ 1

Author(s):

A. Hernandez ◽

D. T. Cooke ◽

D. T. Clarkson

Keyword(s):

Plasma Membrane ◽

Membrane Vesicles ◽

Ustilago Maydis ◽

Plasma Membrane Vesicles ◽

Calcium Accumulation

Download Full-text

Burkholderia cenocepacia transcriptome during the early contacts with giant plasma membrane vesicles derived from live bronchial epithelial cells

Scientific Reports ◽

10.1038/s41598-021-85222-5 ◽

2021 ◽

Vol 11 (1) ◽

Cited By ~ 1

Author(s):

Andreia I. Pimenta ◽

Nuno Bernardes ◽

Marta M. Alves ◽

Dalila Mil-Homens ◽

Arsenio M. Fialho

Keyword(s):

Membrane Vesicles ◽

Bronchial Epithelial Cell ◽

Host Cells ◽

Burkholderia Cenocepacia ◽

Transport Systems ◽

Epithelial Cell Line ◽

Plasma Membrane Vesicles ◽

Bronchial Epithelial ◽

Cellular Processes ◽

Associated Functions

AbstractBurkholderia cenocepacia is known for its capacity of adherence and interaction with the host, causing severe opportunistic lung infections in cystic fibrosis patients. In this work we produced Giant Plasma Membrane Vesicles (GPMVs) from a bronchial epithelial cell line and validated their use as a cell-like alternative to investigate the steps involved in the adhesion process of B. cenocepacia. RNA-sequencing was performed and the analysis of the B. cenocepacia K56-2 transcriptome after the first contacts with the surface of host cells allowed the recognition of genes implicated in bacterial adaptation and virulence-associated functions. The sensing of host membranes led to a transcriptional shift that caused a cascade of metabolic and physiological adaptations to the host specific environment. Many of the differentially expressed genes encode proteins related with central metabolic pathways, transport systems, cellular processes, and virulence traits. The understanding of the changes in gene expression that occur in the early steps of infection can uncover new proteins implicated in B. cenocepacia-host cell adhesion, against which new blocking agents could be designed to control the progression of the infectious process.

Download Full-text