scholarly journals The assessment of the usability of selected instrumental techniques for the elemental analysis of biomedical samples

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Karolina Planeta ◽  
Aldona Kubala-Kukus ◽  
Agnieszka Drozdz ◽  
Katarzyna Matusiak ◽  
Zuzanna Setkowicz ◽  
...  
Keyword(s):  
Elemental Analysis ◽  
Biological Samples ◽  
Inductively Coupled Plasma ◽  
Graphite Furnace ◽  
Detection Limits ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Validation Parameters ◽  
Gf Aas

AbstractThe fundamental role of major, minor and trace elements in different physiological and pathological processes occurring in living organism makes that elemental analysis of biomedical samples becomes more and more popular issue. The most often used tools for analysis of the elemental composition of biological samples include Flame and Graphite Furnace Atomic Absorption Spectroscopy (F-AAS and GF-AAS), Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES) and Inductively Coupled Plasma Mass Spectrometry (ICP-MS). Each of these techniques has many advantages and limitations that should be considered in the first stage of planning the measurement procedure. Their reliability can be checked in the validation process and the precision, trueness and detection limits of elements belong to the most frequently determined validation parameters. The main purpose of this paper was the discussion of selected instrumental techniques (F-AAS, GF-AAS, ICP-OES and ICP-MS) in term of the achieved validation parameters and the usefulness in the analysis of biological samples. The focus in the detailed literature studies was also put on the methods of preparation of the biomedical samples. What is more based on the own data the usefulness of the total reflection X-ray fluorescence spectroscopy for the elemental analysis of animal tissues was examined. The detection limits of elements, precision and trueness for the technique were determined and compared with the literature data concerning other of the discussed techniques of elemental analysis. Reassuming, the following paper is to serve as a guide and comprehensive source of information concerning the validation parameters achievable in different instrumental techniques used for the elemental analysis of biomedical samples.

Evaluation of a custom single Peltier-cooled ablation cell for elemental imaging of biological samples in laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS)

2016 ◽  
Vol 31 (4) ◽  
pp. 1030-1033 ◽  
Author(s):  
J. S. Hamilton ◽  
E. L. Gorishek ◽  
P. M. Mach ◽  
D. Sturtevant ◽  
M. L. Ladage ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Biological Samples ◽  
Inductively Coupled Plasma ◽  
Peltier Element ◽  
Elemental Imaging ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Biological Sampling

A new single Peltier element ablation cell is described and its applicability to biological sampling discussed to evaluate its performance.

scholarly journals Arsenic quantification techniques and ISO/IEC 17025 accreditation in Brazil

2019 ◽  
Vol 6 (14) ◽  
pp. 803-817
Author(s):  
Jefferson Luiz Antunes Santos ◽  
Jader Galba Busato ◽  
Rodrigo de Almeida Heringer ◽  
Juscimar da Silva ◽  
Leonardo Barros Dobbss
Keyword(s):  
Atomic Absorption Spectrometry ◽  
Atomic Absorption ◽  
Inductively Coupled Plasma ◽  
Hydride Generation ◽  
Absorption Spectrometry ◽  
Emission Spectrometry ◽  
Icp Oes ◽  
Analytical Technique ◽  
Inductively Coupled ◽  
Icp Ms

The importance of arsenic (As) quantification in environmental compartments is due to its risks to ecosystems and public health. There are reports of high concentrations of this metalloid in Brazil and technological differences between states are observed. The objective of this work was to present and discuss current scenarios of accreditation and compare the limit of quantification (LOQ) of As by analytical technique in Brazil. Data from accredited laboratories were collected on Inmetro website and in state metrological networks and then grouped and analyzed by state, matrix and analytical technique. There are large discrepancies between the number of laboratories per state and a good correlation with gross domestic product (GDP). Almost all laboratories have a LOQ less than the environmental limits. The observed list of techniques sorted from lowest to highest LOQ values is: for liquid samples ICP MS (inductively coupled plasma mass spectrometry), ET AAS (electrothermal atomic absorption spectrometry), HG AAS (hydride generation combined with atomic absorption spectrometry) or HG ICP OES (hydride generation combined with inductively coupled plasma optical emission spectrometry) and UV VIS (visible ultraviolet spectroscopy); for solids samples HG ICP OES, ICP MS, HG AAS, ET AAS and FAAS (flame atomic absorption spectrometry); and for bioindicators ICP MS, HG ICP OES. Analysis of As species is accredited in only one laboratory, but does not include all species.

scholarly journals Puparial Cases as Toxicological Indicators: Bioaccumulation of Cadmium and Thallium in the Forensically Important Blowfly Lucilia sericata

2020 ◽  
Vol 8 ◽  
Author(s):  
Julita Malejko ◽  
Krzysztof Deoniziak ◽  
Marlena Tomczuk ◽  
Joanna Długokencka ◽  
Beata Godlewska-Żyłkiewicz
Keyword(s):  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Larval Growth ◽  
Lucilia Sericata ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Validation Parameters ◽  
Negative Effect ◽  
Plasma Mass Spectrometry ◽  
Feeding Substrate

In this study, we present entomotoxicological data on the accumulation of cadmium and thallium in a forensically important blowfly, Lucilia sericata, and evaluate the reliability and utility of such information as toxicological evidence for poisoning as a cause of death. We observed that Cd and Tl content in different growing stages of L. sericata (larvae, puparial cases, and adults) was increasing with increasing metal concentration in the feeding substrate, namely metal-enriched liver. However, patterns of accumulation differed between the two metals investigated, showing a linear relationship for Cd and a saturable pattern for Tl. For cadmium, the highest bioaccumulation factor (BAF) was found in the larval stage (in the range of 0.20–0.25), while for thallium, puparial cases accumulated more metal than the other stages tested (BAF in the range of 0.24–0.42). Thallium was also observed to have a negative effect on larval growth, resulting in lower weight and smaller puparial size. With this study, we update the information on the bioaccumulation of cadmium in forensically important blowflies and provide the first report on the bioaccumulation of thallium as well as its developmental impact in blowflies. Specifically, our results suggest that analysis of puparial cases could yield useful information for entomotoxicological investigations. The content of Cd and Tl in larvae, puparial cases, and adults of L. sericata was determined by inductively coupled plasma mass spectrometry (ICP-MS). The validation parameters of the method such as sensitivity, detection limits, quantification limits, precision, and accuracy were evaluated. The method detection limit (MDL) for all types of samples was in the range of 1.6–3.4 ng g−1 for Cd and 0.034–0.15 ng g−1 for Tl, and the accuracy of the method was confirmed by a high recovery of metals from certified reference materials (91.3% for Cd and 94.3% for Tl).

scholarly journals A Comparative Study Between Conventional ICP-OES and the Innovative PLS Model-Assisted ICP-OES for the Assay of Trace Elements

2020 ◽  
Author(s):  
Adel M Michael ◽  
Ahmed A Mohamed ◽  
Yousef A Abdelaziz ◽  
Nesma M Fahmy
Keyword(s):  
Trace Elements ◽  
Elemental Analysis ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Official Method ◽  
Icp Oes ◽  
Inductively Coupled ◽  
Flame Emission ◽  
Significant Difference ◽  
Student’S T

Abstract Background Inductively coupled plasma is widely used for elemental analysis with the advantage of being eco-friendly since the discharge is free of contaminants. Objective A rapid, novel method was developed for the quantitation of trace elements using inductively coupled plasma with optical emission spectrometry.This method has the advantage of simultaneous calibration compared to the conventional method. Method The assay was carried out for iron, copper, zinc, and molybdenum using the linear regression model partial least-squares. Results The method was optimized and validated as per the International Conference on Harmonization guidelines, showing highly accurate and precise results. The linearity range was 0.25–4 ppm for all trace elements under investigation. The method was applied for the assay of the cited elements in non-chelated and amino acid chelated multi-mineral preparations in the Egyptian market with acceptable mean percent recovery. Conclusions In comparison with the official method by flame emission, statistical analysis showed no significant difference with Student’s t-test and F-values. Highlights Inductively coupled plasma is superior as all of the elements can be measured simultaneously. The method was found to have a high degree of specificity and can be easily applied in routine elemental analysis in laboratories.

scholarly journals A Practical and Nontarnishing Method for the Analysis of Trace Nickel in Hydrogenated Cottonseed Oil by Inductively Coupled Plasma/Mass Spectrometry with Pressurized PTFE Vessel Acid Digestion

2010 ◽  
Vol 93 (1) ◽  
pp. 323-326 ◽  
Author(s):  
Ni Zhang ◽  
Zhiying Ding ◽  
Hao Li ◽  
Xin Wang ◽  
Xiaodong Shao
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Graphite Furnace ◽  
Cottonseed Oil ◽  
Atomic Absorption Spectrophotometry ◽  
Acid Digestion ◽  
Inductively Coupled ◽  
Hydrogenated Cottonseed Oil ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract A practical and nontarnishing method for the determination of trace nickel (Ni) in hydrogenated cottonseed oil by inductively coupled plasma/mass spectrometry (ICP/MS) was developed. In order to avoid tarnishing in the pretreatment of samples, the technology of pressurized PTFE vessel acid digestion was applied. The temperature and acid content in the digestion were optimized. The results showed that hydrogenated cottonseed oil could be digested completely by the proposed method. Compared with the U.S. Pharmacopeia 28 and British Pharmacopoeia 2003 methods, the developed method avoided the risk of using platinum and the tarnish from silica crucibles. In addition, the analytical cycle of the test solution was shortened by the use of ICP/MS instead of graphite furnace atomic absorption spectrophotometry.

Advantages, drawbacks and applications of mixed Ar–N2 sources in inductively coupled plasma-based techniques: an overview

2014 ◽  
Vol 6 (16) ◽  
pp. 6170-6182 ◽  
Author(s):  
G. L. Scheffler ◽  
D. Pozebon
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Optical Emission ◽  
Emission Spectrometry ◽  
Icp Oes ◽  
Mixed Gas ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Plasma Optical Emission Spectrometry

This review deals with mixed gas Ar–N2 plasmas, highlighting advantages, limitations and applications of them in inductively coupled plasma optical emission spectrometry (ICP OES), inductively coupled plasma mass spectrometry (ICP-MS) and laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) techniques, covering publications in the last three decades.

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