scholarly journals Puparial Cases as Toxicological Indicators: Bioaccumulation of Cadmium and Thallium in the Forensically Important Blowfly Lucilia sericata

2020 ◽  
Vol 8 ◽  
Author(s):  
Julita Malejko ◽  
Krzysztof Deoniziak ◽  
Marlena Tomczuk ◽  
Joanna Długokencka ◽  
Beata Godlewska-Żyłkiewicz
Keyword(s):  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Larval Growth ◽  
Lucilia Sericata ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Validation Parameters ◽  
Negative Effect ◽  
Plasma Mass Spectrometry ◽  
Feeding Substrate

In this study, we present entomotoxicological data on the accumulation of cadmium and thallium in a forensically important blowfly, Lucilia sericata, and evaluate the reliability and utility of such information as toxicological evidence for poisoning as a cause of death. We observed that Cd and Tl content in different growing stages of L. sericata (larvae, puparial cases, and adults) was increasing with increasing metal concentration in the feeding substrate, namely metal-enriched liver. However, patterns of accumulation differed between the two metals investigated, showing a linear relationship for Cd and a saturable pattern for Tl. For cadmium, the highest bioaccumulation factor (BAF) was found in the larval stage (in the range of 0.20–0.25), while for thallium, puparial cases accumulated more metal than the other stages tested (BAF in the range of 0.24–0.42). Thallium was also observed to have a negative effect on larval growth, resulting in lower weight and smaller puparial size. With this study, we update the information on the bioaccumulation of cadmium in forensically important blowflies and provide the first report on the bioaccumulation of thallium as well as its developmental impact in blowflies. Specifically, our results suggest that analysis of puparial cases could yield useful information for entomotoxicological investigations. The content of Cd and Tl in larvae, puparial cases, and adults of L. sericata was determined by inductively coupled plasma mass spectrometry (ICP-MS). The validation parameters of the method such as sensitivity, detection limits, quantification limits, precision, and accuracy were evaluated. The method detection limit (MDL) for all types of samples was in the range of 1.6–3.4 ng g−1 for Cd and 0.034–0.15 ng g−1 for Tl, and the accuracy of the method was confirmed by a high recovery of metals from certified reference materials (91.3% for Cd and 94.3% for Tl).

Arsenic, antimony and chromium speciation using HPLC-ICP-MS in selected river ecosystems of Upper Silesia, Poland – a preliminary study and validation of methodology

2015 ◽  
Vol 16 (2) ◽  
pp. 354-361 ◽  
Author(s):  
Magdalena Jabłońska-Czapla ◽  
Sebastian Szopa
Keyword(s):  
Inductively Coupled Plasma ◽  
High Performance ◽  
Limit Of Detection ◽  
Saline Water ◽  
Certified Reference Materials ◽  
Upper Silesia ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Full Separation

Upper Silesia is the most transformed by humans of the Polish territories. As a result of mining, urbanization and other industrial activities, irreversible changes have taken place in the environment. In this paper, the method of collecting, preserving, storing and preparing samples from four Silesian rivers was optimized. Silesian sediment and river water samples are a challenge for the analyst, because they have complex matrices and high content of chlorides coming from the discharges of saline water. This study concerned determination of Sb(III), Sb(V), As(III), As(V), MMA, DMA, AB, Cr(III) and Cr(VI) with high-performance liquid chromatography-inductively coupled plasma-mass spectrometry (HPLC-ICP-MS). The following columns were used: Dionex Ion Pack AG7 to determine chromium forms, Hamilton PRP-X100 to separate antimony species and Dionex Ion Pack AS7 to determine five chemical arsenic forms. The methodology used allowed for elimination of interferences during analysis of highly saline and complex samples and ions separation directly without adding any complexing compounds. Depending on the analytes, full separation was obtained within 3–9.5 min. The sediment sample extraction and determination were validated (recovery, limit of detection, repeatability and uncertainty) with the certified reference materials (CRMs). The optimized methodology allowed for study of mobility and seasonality changes of As, Sb and Cr species in the river environment.

DETERMINATION OF LOW CONTENTS (LESS THAN 0,0005%) OF ARSENIC IN NICKEL ALLOYS BY ICP-MS AND AAS METHODS WITH ELECTROTHERMAL ATOMIZATION

2020 ◽  
pp. 3-10
Author(s):  
A.V. Alekseev ◽  
◽  
P.V. Yakimovich ◽  
I.S. Legkodukhova ◽  
◽  
...  
Keyword(s):  
Inductively Coupled Plasma ◽  
Nickel Alloys ◽  
Certified Reference Materials ◽  
Absorption Spectrometry ◽  
Arsenic Content ◽  
Electrothermal Atomization ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

In this work, the determination of low contents (less than 0,0005 wt. %) of arsenic in complex alloyed samples of nickel alloys was carried out by means of inductively coupled plasma mass spectrometry (ICP-MS) and atomic absorption spectrometry with electrothermal atomization. Also, the ICP-MS method was used to determine the arsenic content in chromium, which is an alloying component of nickel alloys. A technique for dissolving a sample and preparing it for analysis is presented. Spectral interferences are eliminated by applying mathematical correction equations, a reaction-collision cell and using corrective additives. The correctness of the results obtained is confirmed by the analysis of certified reference materials of nickel alloys and chromium.

scholarly journals Accurate measurement of uranium isotope ratios in solid samples by laser ablation multi-collector inductively coupled plasma mass spectrometry

2018 ◽  
Vol 33 (6) ◽  
pp. 1076-1080 ◽  
Author(s):  
Z. Varga ◽  
M. Krachler ◽  
A. Nicholl ◽  
M. Ernstberger ◽  
T. Wiss ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Laser Ablation ◽  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Isotope Ratios ◽  
Uranium Isotope ◽  
Solid Samples ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

A multi-collector inductively coupled plasma mass spectrometer (MC-ICP-MS) coupled to a 213 nm ns-laser was used to measure uranium isotope ratios (234U/238U, 235U/238U, and 236U/238U) in six solid nuclear certified reference materials (CRMs).

scholarly journals Optimized Simultaneous Determination of Several Elements in Human Intestinal Caco-2 TC7 Cells by Inductively Coupled Plasma-Mass Spectrometry after Closed Vessel Microwave Digestion

2003 ◽  
Vol 86 (6) ◽  
pp. 1225-1231 ◽  
Author(s):  
Laurent Noël ◽  
Thierry Guérin ◽  
Jean-Marc Frémy ◽  
Hélène Huet ◽  
Martine Kolf-Clauw
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Microwave Digestion ◽  
Absorption Spectrometry ◽  
Inductively Coupled ◽  
Flame Atomic Absorption ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract A rapid procedure, based on closed vessels microwave digestion and inductively coupled plasma-mass spectrometry (ICP-MS), was evaluated to ascertain the effect of chronic exposure to cadmium on intracellular accumulation of minor and essential trace elements in cultured epithelial cells (Caco-2 TC7). For all measurements, the method of external calibration was used and 3 elements (Be, Sc, In) were selected as internal standards. Optimization procedures are discussed and results are presented for the total determination of 9 key analytes (Na, Mg, Ca, Cr, Mn, Cu, Zn, Mo, and Cd) in certified reference materials (CRMs) and 20 samples of Caco-2 TC7 cells long-term exposed to Cd. The performance characteristics of the analytical system were evaluated by calibration and linearity, limits of detection and quantitation, accuracy with spiking, trueness and repeatability with available CRMs. As a complement to the ICP-MS determinations, both available CRMs and cell samples were analyzed either by electro thermal-or flame atomic absorption spectrometry. The results were in good agreement with the ICP-MS results.

scholarly journals Development and Validation of an Inductively Coupled Plasma Mass Spectrometry (ICP-MS) Method for Quantitative Analysis of Platinum in Plasma, Urine, and Tissues

2016 ◽  
Vol 70 (9) ◽  
pp. 1529-1536 ◽  
Author(s):  
Ti Zhang ◽  
Shuang Cai ◽  
Wai Chee Forrest ◽  
Eva Mohr ◽  
Qiuhong Yang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Reaction System ◽  
Chemotherapeutic Agents ◽  
Limit Of Quantification ◽  
Rat Urine ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Validation Parameters ◽  
Plasma Mass Spectrometry

Cisplatin, a platinum chemotherapeutic, is one of the most commonly used chemotherapeutic agents for many solid tumors. In this work, we developed and validated an inductively coupled plasma mass spectrometry (ICP-MS) method for quantitative determination of platinum levels in rat urine, plasma, and tissue matrices including liver, brain, lungs, kidney, muscle, heart, spleen, bladder, and lymph nodes. The tissues were processed using a microwave accelerated reaction system (MARS) system prior to analysis on an Agilent 7500 ICP-MS. According to the Food and Drug Administration guidance for industry, bioanalytical validation parameters of the method, such as selectivity, accuracy, precision, recovery, and stability were evaluated in rat biological samples. Our data suggested that the method was selective for platinum without interferences caused by other presenting elements, and the lower limit of quantification was 0.5 ppb. The accuracy and precision of the method were within 15% variation and the recoveries of platinum for all tissue matrices examined were determined to be 85–115% of the theoretical values. The stability of the platinum-containing solutions, including calibration standards, stock solutions, and processed samples in rat biological matrices was investigated. Results indicated that the samples were stable after three cycles of freeze–thaw and for up to three months.

scholarly journals Determination of Some Elements in Legumes Using ICP-MS and EDXRF Methodology Applications

2021 ◽  
Vol 68 (4) ◽  
pp. 913-920
Author(s):  
Lovro Sinkovič ◽  
Marijan Nečemer ◽  
Barbara Pipan ◽  
Vladimir Meglič
Keyword(s):  
Elemental Composition ◽  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Principal Component ◽  
Inductively Coupled ◽  
Cu Content ◽  
Icp Ms ◽  
Plasma Mass Spectrometry ◽  
Analytical Research

The current study involves two analytical research techniques, inductively coupled plasma-mass spectrometry (ICP-MS) and energy dispersive X-ray fluorescence (EDXRF) spectroscopy, used to determine the elemental composition of different legumes usually produced and consumed in Slovenia. Results indicate that data obtained using these methods are in agreement with certified reference materials. In total, nineteen elements were determined from twenty legume samples. An intercomparison between four macro- (P, S, K, Ca) and three microelements (Fe, Zn, Mo) measured using ICP-MS and EDXRF methods showed a strong correlation. The EDXRF was found to be a cheaper, simpler and more environmentally friendly method for determination of elements P, S, Cl, K, Ca, Fe, Zn, Mo, Sr, Rb, Ti and Br in legumes, while for the identification and determination of Na, Mg, V, Cr, Mn, Co and Cu content ICP‐MS was the method of choice due to its excellent sensitivity and accuracy. Using principal component analysis (PCA), the samples of the studied legumes were classified into four groups according to their elemental composition.

scholarly journals Quantitative aspects of inductively coupled plasma mass spectrometry

2016 ◽  
Vol 374 (2079) ◽  
pp. 20150369 ◽  
Author(s):  
Ewa Bulska ◽  
Barbara Wagner
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Environmental Science ◽  
Certified Reference Materials ◽  
Accurate Determination ◽  
High Sensitivity ◽  
Good Precision ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Accurate determination of elements in various kinds of samples is essential for many areas, including environmental science, medicine, as well as industry. Inductively coupled plasma mass spectrometry (ICP-MS) is a powerful tool enabling multi-elemental analysis of numerous matrices with high sensitivity and good precision. Various calibration approaches can be used to perform accurate quantitative measurements by ICP-MS. They include the use of pure standards, matrix-matched standards, or relevant certified reference materials, assuring traceability of the reported results. This review critically evaluates the advantages and limitations of different calibration approaches, which are used in quantitative analyses by ICP-MS. Examples of such analyses are provided. This article is part of the themed issue ‘Quantitative mass spectrometry’.

scholarly journals Two new separation schemes for the group isolation of rare earth elements (REE) from biological and other matrices and their determination by ICP-MS, NAA and chromatographic methods

Nukleonika ◽  
2017 ◽  
Vol 62 (3) ◽  
pp. 199-211 ◽  
Author(s):  
Rajmund S. Dybczyński ◽  
Zbigniew Samczyński ◽  
Iwona Bartosiewicz ◽  
Krzysztof Kulisa ◽  
Halina Polkowska-Motrenko ◽  
...  
Keyword(s):  
Rare Earth ◽  
Ion Exchange ◽  
Rare Earth Elements ◽  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Ion Exchange Chromatography ◽  
Exchange Chromatography ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Plasma Mass Spectrometry

Abstract Two new group separation schemes, based on ion exchange chromatography, for the selective and quantitative isolation of rare earth elements (REE) from accompanying elements, were devised. After checking their performance with the aid of radioactive tracers, the schemes were further used together with ICP-MS, NAA and ion exchange chromatography for the determination of Sc, Y, La, Ce, Pr, Nd, Sm, Eu, Gd, Tb, Dy, Ho, Er, Tm, Yb and Lu in two certified reference materials (CRMs). The results were compared with another series of analyses, where the REEs were determined directly, i.e. without pre-separation, by instrumental neutron activation analysis (INAA) and inductively coupled plasma mass spectrometry (ICP-MS). It was demonstrated that while direct INAA and ICP-MS in most instances provide reliable results for the majority of REEs, for some elements, notably Sc, Yb and Tm in the cases of ICP-MS and INAA, respectively, systematic errors occur or may potentially occur.

scholarly journals Comparison of Ion-Selective Electrode and Inductively Coupled Plasma-Mass Spectrometry to Determine Iodine in Milk-Based Nutritional Products

2008 ◽  
Vol 91 (6) ◽  
pp. 1397-1401 ◽  
Author(s):  
Daniel Hammer ◽  
Daniel Andrey
Keyword(s):  
Mass Spectrometry ◽  
Inductively Coupled Plasma ◽  
Certified Reference Materials ◽  
Ion Selective Electrode ◽  
Good Precision ◽  
Selective Electrode ◽  
Inductively Coupled ◽  
Icp Ms ◽  
Relative Standard ◽  
Plasma Mass Spectrometry

Abstract The performances of 2 official methods for iodine analysis based on inductively coupled plasma-mass spectrometry (ICP-MS) and the ion-selective electrode (ISE) method were compared for milk-based products. The aim of the study was to determine the performance characteristics of both methods to check the labeled concentration of iodine. Good precision was found for both methods with highest relative standard deviation of repeatability (RSDr) at 2.3 and 2.7 for ISE and ICP-MS, respectively. Intermediate reproducibility (RSDiR), single laboratory within 6 different days, was also good with the highest values at 7.3 and 8 by ISE and ICP-MS, respectively. Measurement uncertainty was estimated based on the RSDiR data, and it was concluded that both methods were capable of determining iodine concentrations within an uncertainty below 20. The accuracy of the methods was determined by analyzing certified reference materials, in-house proficiency test samples, and commercial products. Both methods returned similar results when applied on freshly opened samples. In samples that had been opened and kept exposed to air during storage, ISE returned lower iodine concentrations than ICP-MS. In commercial samples, the linear regression between both methods was ISE 0.95 ICP-MS 0.060 for freshly opened samples and ISE 0.85 ICP-MS 0.069 for samples exposed to air. The tendency of ISE to return lower results than ICP-MS is explained by the fact that ISE is sensitive to iodide but does not measure iodine that may be bound organically to the matrix. This seems to be more pronounced in samples that were stored longer. Because in most countries iodine is labeled as total iodine, acceptance of an international standard based on the ICP-MS technique which takes all forms of iodine into account, is recommended. This would help to avoid any potential dispute on the accuracy of labeled iodine concentrations in finished products.

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