scholarly journals Draft genome sequence of the pulse crop blackgram [Vigna mungo (L.) Hepper] reveals potential R-genes

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Souframanien Jegadeesan ◽  
Avi Raizada ◽  
Punniyamoorthy Dhanasekar ◽  
Penna Suprasanna
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Vigna Mungo ◽  
Draft Genome Sequence ◽  
Sequence Length ◽  
Legume Crop ◽  
Oxford Nanopore ◽  
Hybrid Genome ◽  
First Time

AbstractBlackgram [Vigna mungo (L.) Hepper] (2n = 2x = 22), an important Asiatic legume crop, is a major source of dietary protein for the predominantly vegetarian population. Here we construct a draft genome sequence of blackgram, for the first time, by employing hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. The final de novo whole genome of blackgram is ~ 475 Mb (82% of the genome) and has maximum scaffold length of 6.3 Mb with scaffold N50 of 1.42 Mb. Genome analysis identified 42,115 genes with mean coding sequence length of 1131 bp. Around 80.6% of predicted genes were annotated. Nearly half of the assembled sequence is composed of repetitive elements with retrotransposons as major (47.3% of genome) transposable elements, whereas, DNA transposons made up only 2.29% of the genome. A total of 166,014 SSRs, including 65,180 compound SSRs, were identified and primer pairs for 34,816 SSRs were designed. Out of the 33,959 proteins, 1659 proteins showed presence of R-gene related domains. KIN class was found in majority of the proteins (905) followed by RLK (239) and RLP (188). The genome sequence of blackgram will facilitate identification of agronomically important genes and accelerate the genetic improvement of blackgram.

scholarly journals Draft genome sequence of the pulse crop blackgram [Vigna mungo (L.) Hepper] reveals potential R-genes

2020 ◽  
Author(s):  
J Souframanien ◽  
Avi Raizada ◽  
Punniyamoorthy Dhanasekar ◽  
Penna Suprasanna
Keyword(s):  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Vigna Mungo ◽  
Draft Genome Sequence ◽  
Sequence Length ◽  
Legume Crop ◽  
Oxford Nanopore ◽  
Hybrid Genome ◽  
First Time

AbstractBlackgram [Vigna mungo (L.) Hepper] (2n = 2x = 22), an important Asiatic legume crop, is a major source of dietary protein for the predominantly vegetarian population. Here we construct a draft genome sequence of blackgram, for the first time, by employing hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. The final de novo whole genome of blackgram is ~ 475 Mb (82 % of the genome) and has maximum scaffold length of 6.3 Mb with scaffold N50 of1.42 Mb. Genome analysis identified 18655 genes with mean coding sequence length of 970bp. Around 96.7 % of predicted genes were annotated. Nearly half of the assembled sequence is composed of repetitive elements with retrotransposons as major (47.3% of genome) transposable elements, whereas, DNA transposons made up only 2.29% of the genome. A total of 166014 SSRs, including 65180 compound SSRs, were identified and primer pairs for 34816 SSRs were designed. Out of the 18665 proteins, 678 proteins showed presence of R-gene related domains. KIN class was found in majority of the proteins (372) followed by RLK (79) and N (79). The genome sequence of blackgram will facilitate identification of agronomically important genes and accelerate the genetic improvement of blackgram.

scholarly journals First de novo draft genome sequence of Oryza coarctata, the only halophytic species in the genus Oryza

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1750 ◽  
Author(s):  
Tapan Kumar Mondal ◽  
Hukam Chand Rawal ◽  
Kishor Gaikwad ◽  
Tilak Raj Sharma ◽  
Nagendra Kumar Singh
Keyword(s):  
West Bengal ◽  
De Novo ◽  
Draft Genome ◽  
Nanopore Sequencing ◽  
Sequencing Technology ◽  
Genome Sequences ◽  
Oxford Nanopore ◽  
Hybrid Genome ◽  
Genus Oryza ◽  
First Time

Oryza coarctata plants, collected from Sundarban delta of West Bengal, India, have been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. We report for the first time that more than 85.71 % of the genome coverage and the data have been deposited in NCBI SRA, with BioProject ID PRJNA396417.

scholarly journals First de novo draft genome sequence of Oryza coarctata, the only halophytic species in the genus Oryza

F1000Research ◽  
2017 ◽  
Vol 6 ◽  
pp. 1750 ◽  
Author(s):  
Tapan Kumar Mondal ◽  
Hukam Chand Rawal ◽  
Kishor Gaikwad ◽  
Tilak Raj Sharma ◽  
Nagendra Kumar Singh
Keyword(s):  
West Bengal ◽  
De Novo ◽  
Draft Genome ◽  
Nanopore Sequencing ◽  
Sequencing Technology ◽  
Genome Sequences ◽  
Oxford Nanopore ◽  
Hybrid Genome ◽  
Genus Oryza ◽  
First Time

Oryza coarctata plant, collected from Sundarban delta of West Bengal, India,  has been used in the present study to generate draft genome sequences, employing the hybrid genome assembly with Illumina reads and third generation Oxford Nanopore sequencing technology. We report for the first time the draft genome with the coverage of 85.71 %  and  deposited the raw data in NCBI SRA, with BioProject ID PRJNA396417.

scholarly journals Draft Genome Sequence of Pantoea sp. Strain A4, a Rafflesia-Associated Bacterium That ProducesN-Acylhomoserine Lactones as Quorum-Sensing Molecules

2012 ◽  
Vol 194 (23) ◽  
pp. 6610-6610 ◽  
Author(s):  
Kar-Wai Hong ◽  
Han Ming Gan ◽  
Siew-Moon Low ◽  
Patrick Kok Yuen Lee ◽  
Yee-Meng Chong ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Negative Bacterium ◽  
Gram Negative ◽  
Content Type ◽  
First Time

ABSTRACTPantoeasp. strain A4 is a Gram-negative bacterium isolated from theRafflesiaflower. We present here, for the first time, the genome sequence ofRafflesia-associatedPantoeasp. strain A4, which exhibited quorum-sensing activity.

scholarly journals Draft Genome Sequence of Leifsonia xyli subsp. xyli Strain gdw1

2016 ◽  
Vol 4 (5) ◽  
Author(s):  
Jihua Wang ◽  
Li Wang ◽  
Gan Cao ◽  
Muqing Zhang ◽  
Ying Guo
Keyword(s):  
Genome Sequence ◽  
Genetic Improvement ◽  
De Novo ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Coding Sequences ◽  
Ratoon Stunting Disease

Here, we report the draft genome sequence of Leifsonia xyli subsp. xyli strain gdw1, isolated from the stem of Badila sugarcane located at the Guangdong Key Laboratory for Crops Genetic Improvement (Guanzhou, China), that causes ratoon stunting disease of sugarcane. The de novo genome of Leifsonia xyli subsp. xyli was assembled with 48 scaffolds and a G+C content of 67.68%, and contained 2.6 Mb bp and 2,838 coding sequences.

scholarly journals Draft Genome Sequence of the Community-Associated Staphylococcus aureus Sequence Type 88 Strain LVP-7, Isolated from an Ocular Infection

2021 ◽  
Vol 10 (7) ◽  
Author(s):  
Savitha Nadig ◽  
Sneha Murthy ◽  
Muralidharan Vandanashree ◽  
Hosahalli S. Subramanya ◽  
Balasubramanian Gopal ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Sequence Type ◽  
Draft Genome Sequence ◽  
Ocular Infection ◽  
Content Type ◽  
Type V ◽  
Panton Valentine Leukocidin

ABSTRACT We report a de novo-assembled draft genome sequence of the Indian Staphylococcus aureus sequence type 88 (ST88) strain LVP-7, isolated from an ocular infection. The genome harbors a Panton-Valentine leukocidin phage, a type V staphylococcal cassette chromosome mec element, the delta-hemolysin-converting Newman phage ΦNM3, and the pathogenicity island SaPI3, encoding the superantigen enterotoxin B.

scholarly journals Draft Genome Sequence of a New Zealand Isolate of Mycoplasma ovipneumoniae

2020 ◽  
Vol 9 (10) ◽  
Author(s):  
B. J. Bridgeman ◽  
S. K. Gupta ◽  
A. Murray ◽  
V. S. R. Dukkipati ◽  
E. Altermann ◽  
...  
Keyword(s):  
New Zealand ◽  
Genome Sequence ◽  
De Novo ◽  
Draft Genome ◽  
Illumina Miseq ◽  
Draft Genome Sequence ◽  
Content Type ◽  
Mycoplasma Ovipneumoniae

The genome of New Zealand Mycoplasma ovipneumoniae isolate 90 was sequenced and assembled using an Illumina MiSeq system and combining the built-in Geneious de novo and Velvet de novo assemblers. The 1,031,345-bp-long genome harbored 711 genes with a coding percentage of 86.6.

scholarly journals First Draft Genome of the Trypanosomatid Herpetomonas muscarum ingenoplastis through MinION Oxford Nanopore Technology and Illumina Sequencing

2020 ◽  
Vol 5 (1) ◽  
pp. 25
Author(s):  
Claudia M. d’Avila-Levy ◽  
Bertrand Bearzatto ◽  
Jérôme Ambroise ◽  
Raphaël Helaers ◽  
Anzhelika Butenko ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Illumina Sequencing ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Phormia Regina ◽  
Oxford Nanopore

Here, we present first draft genome sequence of the trypanosomatid Herpetomonas muscarum ingenoplastis. This parasite was isolated repeatedly in the black blowfly, Phormia regina, and it forms a phylogenetically distinct clade in the Trypanosomatidae family.

scholarly journals Improved De Novo Draft Genome Sequence of the Nocavionin-Producing Type Strain Nocardia terpenica IFM 0706 and Comparative Genomics with the Closely Related Strain Nocardia terpenica IFM 0406

2020 ◽  
Vol 9 (34) ◽  
Author(s):  
Anina Buchmann ◽  
Harald Gross
Keyword(s):  
Comparative Genomics ◽  
Genome Size ◽  
Genome Sequence ◽  
Type Strain ◽  
De Novo ◽  
Draft Genome ◽  
Pathogenic Strain ◽  
Draft Genome Sequence ◽  
Related Strain ◽  
Content Type

ABSTRACT We report an improved de novo draft genome sequence of the human-pathogenic strain Nocardia terpenica IFM 0706T. The resequencing unveiled that the genome size is larger than anticipated, reducing significantly the number of contigs and building a basis for comparison with the closely related strain N. terpenica IFM 0406.

scholarly journals Draft Genome Sequence of Ralstonia sp. MD27, a Poly(3-Hydroxybutyrate)-Degrading Bacterium, Isolated from Compost

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Morgan Zhu ◽  
Lucy M. McCully ◽  
Mark W. Silby ◽  
Tamunonengiyeofori I. Charles-Ogan ◽  
Jean Huang ◽  
...  
Keyword(s):  
Carbon Source ◽  
Genome Sequence ◽  
Draft Genome ◽  
Draft Genome Sequence ◽  
Sequence Length ◽  
Total Sequence ◽  
Degrading Bacterium ◽  
Total Sequence Length

Ralstonia sp. strain MD27, a novel biopolymer-degrading betaproteobacterium, was isolated from compost samples. This organism has been shown to utilize the biopolymer poly(3-hydroxybutyrate) [P(3HB)] as a carbon source for growth. We report the draft genome sequence of MD27 with an estimated total sequence length of 5.9 Mb.

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