Examining the effects of cigarette smoke on mouse lens through a multi OMIC approach

AbstractHere, we report a multi OMIC (transcriptome, proteome, and metabolome) approach to investigate molecular changes in lens fiber cells (FC) of mice exposed to cigarette smoke (CS). Pregnant mice were placed in a whole-body smoke chamber and a few days later pups were born, which were exposed to CS for 5 hours/day, 5 days/week for a total of 3½ months. We examined the mice exposed to CS for CS-related cataractogenesis after completion of the CS exposure but no cataracts were observed. Lenses of CS-exposed and age-matched, untreated control mice were extracted and lens FC were subjected to multi OMIC profiling. We identified 348 genes, 130 proteins, and 14 metabolites exhibiting significant (p < 0.05) differential levels in lens FC of mice exposed to CS, corresponding to 3.6%, 4.3%, and 5.0% of the total genes, protein, and metabolites, respectively identified in this study. Our multi OMIC approach confirmed that only a small fraction of the transcriptome, the proteome, and the metabolome was perturbed in the lens FC of mice exposed to CS, which suggests that exposure of CS had a minimal effect on the mouse lens. It is worth noting that while our results confirm that CS exposure does not have a substantial impact on the molecular landscape of the mouse lens FC, we cannot rule out that CS exposure for longer durations and/or in combination with other morbidities or environmental factors would have a more robust effect and/or result in cataractogenesis.

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Cigarette exposure induces changes in maternal vascular function in a pregnant mouse model

AJP Regulatory Integrative and Comparative Physiology ◽

10.1152/ajpregu.00274.2009 ◽

2010 ◽

Vol 298 (5) ◽

pp. R1249-R1256 ◽

Cited By ~ 9

Author(s):

Robin E. Gandley ◽

Arun Jeyabalan ◽

Ketaki Desai ◽

Stacy McGonigal ◽

Jennifer Rohland ◽

...

Keyword(s):

Mouse Model ◽

Cigarette Smoke ◽

Fetal Growth ◽

Fetal Growth Restriction ◽

Smoke Exposure ◽

Renal Arteries ◽

Growth Restriction ◽

Whole Body ◽

Cigarette Smoke Exposure ◽

Pregnant Mice

Smoking is associated with multiple adverse pregnancy outcomes, including fetal growth restriction. The objective of this study was to determine whether cigarette smoke exposure during pregnancy in a mouse model affects the functional properties of maternal uterine, mesenteric, and renal arteries as a possible mechanism for growth restriction. C57Bl/CJ mice were exposed to whole body sidestream smoke for 4 h/day. Smoke particle exposure was increased from day 4 of gestation until late pregnancy ( day 16–19), with mean total suspended particle levels of 63 mg/m3, representative of moderate-to-heavy smoking in humans. Uterine, mesenteric, and renal arteries from late-pregnant and virgin mice were isolated and studied in a pressure-arteriograph system ( n = 23). Plasma cotinine was measured by ELISA. Fetal weights were significantly reduced in smoke-exposed compared with control fetuses (0.88 ± 0.1 vs. 1.0 ± 0.08 g, P < 0.02), while litter sizes were not different. Endothelium-mediated relaxation responses to methacholine were significantly impaired in both the uterine and mesenteric vasculature of pregnant mice exposed to cigarette smoke during gestation. This difference was not apparent in isolated renal arteries from pregnant mice exposed to cigarette smoke; however, relaxation was significantly reduced in renal arteries from smoke-exposed virgin mice. In conclusion, we found that passive cigarette smoke exposure is associated with impaired vascular relaxation of uterine and mesenteric arteries in pregnant mice. Functional maternal vascular perturbations during pregnancy, specifically impaired peripheral and uterine vasodilation, may contribute to a mechanism by which smoking results in fetal growth restriction.

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Prenatal cigarette smoke exposure slightly alters neurobehavioral development in neonatal rats: Implications for developmental origins of health and disease (DoHAD)

Physiology International ◽

10.1556/2060.2020.00007 ◽

2020 ◽

Vol 107 (1) ◽

pp. 55-66

Author(s):

B. Mammel ◽

T. Kvárik ◽

Zs. Szabó ◽

J. Gyarmati ◽

T. Ertl ◽

...

Keyword(s):

Cigarette Smoke ◽

Motor Coordination ◽

Smoke Exposure ◽

Whole Body ◽

Cigarette Smoke Exposure ◽

Control Group ◽

Developmental Origins ◽

Smoking During Pregnancy ◽

Neurobehavioral Development ◽

Health And Disease

AbstractNumerous studies indicate that smoking during pregnancy exerts harmful effects on fetal brain development. The aim of this study was to determine the influence of maternal smoking during pregnancy on the early physical and neurobehavioral development of newborn rats. Wistar rats were subjected to whole-body smoke exposure for 2 × 40 min daily from the day of mating until day of delivery. For this treatment, a manual closed-chamber smoking system and 4 research cigarettes per occasion were used. After delivery the offspring were tested daily for somatic growth, maturation of facial characteristics and neurobehavioral development until three weeks of age. Motor coordination tests were performed at 3 and 4 weeks of age. We found that prenatal cigarette smoke exposure did not alter weight gain or motor coordination. Critical physical reflexes indicative of neurobehavioral development (eyelid reflex, ear unfolding) appeared significantly later in pups prenatally exposed to smoke as compared to the control group. Prenatal smoke exposure also resulted in a delayed appearance of reflexes indicating neural maturity, including hind limb grasping and forelimb placing reflexes. In conclusion, clinically relevant prenatal exposure to cigarette smoke results in slightly altered neurobehavioral development in rat pups. These findings suggest that chronic exposure of pregnant mothers to cigarette smoke (including passive smoking) results in persisting alterations in the developing brain, which may have long-lasting consequences supporting the concept of developmental origins of health and disease (DoHAD).

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Distribution in Pregnant Mice of Radioactivity after Injection of 131I, and Immunosuppressive Effect by the Whole Body Irradiation

Japanese journal of leprosy ◽

10.5025/hansen1977.47.7 ◽

1978 ◽

Vol 47 (1) ◽

pp. 7-12

Author(s):

KIYO SUSHIDA ◽

HISAO NAKANO

Keyword(s):

Immunosuppressive Effect ◽

Whole Body ◽

Pregnant Mice

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Blut-Hirn-Schranke und Placentar-Schranke für H-Thymidin und H-Cytidin bei der Maus / Blood-Brain-Barrier and Placental-Barrier for H-Thymidine and H-Cytidine in the Mouse

Zeitschrift für Naturforschung B ◽

10.1515/znb-1972-0516 ◽

1972 ◽

Vol 27 (5) ◽

pp. 554-558b ◽

Cited By ~ 10

Author(s):

B. Schultze ◽

N. Hörning ◽

W. Maurer

Keyword(s):

Blood Brain Barrier ◽

Intravenous Injection ◽

Brain Barrier ◽

Whole Body ◽

Placental Barrier ◽

Whole Body Autoradiography ◽

Blood Brain ◽

Pregnant Mice ◽

Distribution In The Organism

The distribution in the organism of the mouse of free 3H-thymidine and 3H-cytidine was studied 1, 2½, 5 and 15 minutes after intravenous injection into normal and pregnant mice (20th day) using whole body autoradiography. The grain density measured over brain and fetus is 10 times smaller as compared to other tissues of the organism. This means that a blood brain barrier and placental barrier for thymidine and cytidine exists.

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Whole-body retention and fetal uptake of 65Zn in pregnant mice fed a Zn-deficient diet.

Journal of Radiation Research ◽

10.1269/jrr.36.196 ◽

1995 ◽

Vol 36 (3) ◽

pp. 196-202 ◽

Cited By ~ 1

Author(s):

NAONORI MATSUSAKA ◽

HIDEKI SAKAMOTO ◽

ITARU SATO ◽

KUNIHIRO SHINAGAWA ◽

HARUO KOBAYASHI ◽

...

Keyword(s):

Whole Body ◽

Deficient Diet ◽

Body Retention ◽

Pregnant Mice ◽

Fetal Uptake

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Unusual presentation of late-onset disseminated staphylococcal sepsis in a preterm infant

BMJ Case Reports ◽

10.1136/bcr-2018-226325 ◽

2019 ◽

Vol 12 (3) ◽

pp. e226325 ◽

Cited By ~ 1

Author(s):

Shahzad Gul Khattak ◽

Ian Dady ◽

Devdeep Mukherjee

Keyword(s):

Late Onset ◽

Whole Body ◽

Whole Body Mri ◽

Left Hand ◽

Non Invasive ◽

Male Baby ◽

Left Elbow ◽

The Right ◽

Panton Valentine Leukocidin ◽

Rule Out

An ex-30-week gestation, preterm male baby was admitted to a tertiary neonatal unit and noted to have increased ventilator requirements and diagnosed with sepsis. The baby also developed an abscess over the left elbow and over the xiphisternum along with a decrease in movement of the left hand and the right leg. Panton-Valentine leukocidin (PVL)-producing Staphylococcus aureus (SA) was isolated from the blood culture. A whole body MRI showed disseminated abscess with multiple foci in the lung, left elbow and over the xiphisternum. Disseminated sepsis with multiple septic foci has not been previously reported in neonates. We would like to highlight the fact that sepsis due to PVL toxin-producing SA can cause significant morbidity and mortality in neonates. Proper screening should be done to rule out septic foci in neonates. MRI is a good non-invasive investigation to document septic foci in a neonate and rule out multiorgan involvement.

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Systems toxicology approaches enable mechanistic comparison of spontaneous and cigarette smoke-related lung tumor development in the A/J mouse model

Interdisciplinary Toxicology ◽

10.2478/intox-2014-0010 ◽

2014 ◽

Vol 7 (2) ◽

pp. 73-84 ◽

Cited By ~ 9

Author(s):

Karsta Luettich ◽

Yang Xiang ◽

Anita Iskandar ◽

Alain Sewer ◽

Florian Martin ◽

...

Keyword(s):

Gene Expression ◽

Mouse Model ◽

Cigarette Smoke ◽

Lung Tumor ◽

Immune Surveillance ◽

Gene Expression Signature ◽

Chemical Carcinogenesis ◽

Lung Tumors ◽

Whole Body ◽

Significant Differential Expression

ABSTRACT The A/J mouse is highly susceptible to lung tumor induction and has been widely used as a screening model in carcinogenicity testing and chemoprevention studies. However, the A/J mouse model has several disadvantages. Most notably, it develops lung tumors spontaneously. Moreover, there is a considerable gap in our understanding of the underlying mechanisms of pulmonary chemical carcinogenesis in the A/J mouse. Therefore, we examined the differences between spontaneous and cigarette smokerelated lung tumors in the A/J mouse model using mRNA and microRNA (miRNA) profiling. Male A/J mice were exposed whole-body to mainstream cigarette smoke (MS) for 18 months. Gene expression interaction term analysis of lung tumors and surrounding nontumorous parenchyma samples from animals that were exposed to either 300 mg/m3 MS or sham-exposed to fresh air indicated significant differential expression of 296 genes. Ingenuity Pathway Analysis® (IPA®) indicated an overall suppression of the humoral immune response, which was accompanied by a disruption of sphingolipid and glycosaminoglycan metabolism and a deregulation of potentially oncogenic miRNA in tumors of MS-exposed A/J mice. Thus, we propose that MS exposure leads to severe perturbations in pathways essential for tumor recognition by the immune system, thereby potentiating the ability of tumor cells to escape from immune surveillance. Further, exposure to MS appeared to affect expression of miRNA, which have previously been implicated in carcinogenesis and are thought to contribute to tumor progression. Finally, we identified a 50-gene expression signature and show its utility in distinguishing between cigarette smoke-related and spontaneous lung tumors

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