scholarly journals Control of axillary bud growth in tobacco through toxin gene expression system

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Jing Lv ◽  
Ya-Qiong Chen ◽  
An-Ming Ding ◽  
Bo Lei ◽  
Jing Yu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Axillary Bud ◽  
Toxin Gene ◽  
Biological Traits ◽  
Chemical Induction ◽  
Control Of Gene Expression ◽  
Bud Growth ◽  
Axillary Bud Growth ◽  
Terminal Buds

AbstractThe control of axillary bud development after removing the terminal buds (topping) of plants is a research hotspot, and the control of gene expression, like switching on and off, allows us to further study biological traits of interest, such as plant branching and fertility. In this study, a toxin gene control system for plants based on dexamethasone (DEX) induction was constructed, and the positive transgenic tobacco exhibited growth retardation in the application area (axillary bud). The expression level of the lethal Diphtheria toxin A (DTA) gene under different DEX concentrations at different application days was analyzed. The highest expression levels appeared at 5 days after the leaf injection of DEX. The DTA transcripts were induced by 5 µM DEX and peaked in response to 50 µM DEX at 5 days after leaf injection. Here, a chemical induction system, combined with a toxin gene, were used to successfully control the growth of tobacco axillary buds after topping. The DTA expression system under DEX induction was sensitive and efficient, therefore, can be used to control axillary bud growth and development in tobacco.

Sucrose Mobilisation in Sugarcane Stalk Induced by Heterotrophic Axillary Bud Growth

2012 ◽  
Vol 5 (2) ◽  
pp. 173-182 ◽  
Author(s):  
Brian P. O’Neill ◽  
Matthew P. Purnell ◽  
David J. Anderson ◽  
Lars K. Nielsen ◽  
Stevens M. Brumbley
Keyword(s):  
Axillary Bud ◽  
Sugarcane Stalk ◽  
Bud Growth ◽  
Axillary Bud Growth ◽  
Sucrose Mobilisation

scholarly journals Transcriptomic and physiological analyses of rice seedlings under different nitrogen supplies provide insight into the regulation involved in axillary bud outgrowth

2020 ◽  
Author(s):  
Rongna Wang ◽  
Junjie Qian ◽  
Zhongming Fang ◽  
Jihua Tang
Keyword(s):  
Growth Rate ◽  
Morphological Changes ◽  
Expression Profiles ◽  
Axillary Buds ◽  
Axillary Bud ◽  
Rice Seedlings ◽  
Transcriptomic Data ◽  
Bud Growth ◽  
Axillary Bud Growth ◽  
Axillary Bud Outgrowth

Abstract Background: N is an important macronutrient required for plant development and significantly influences axillary bud outgrowth, which affects tillering and grain yields of rice. However, how different N concentrations affect axillary bud growth at the molecular and transcriptional levels remains unclear. Results: In this study, morphological changes in the axillary bud growth of rice seedlings under different N concentrations ranging from low to high levels were systematically observed. To investigate the expression of N-induced genes involved in axillary bud growth, we used RNA-seq technology to generate mRNA transcriptomic data from two tissue types, basal parts and axillary buds, of plants grown under six different N concentrations. In total, 10,221 and 12,180 DEGs induced by LN or HN supplies were identified in the basal parts and axillary buds, respectively, via comparisons to expression levels under NN level. Analysis of the coexpression modules from the DEGs of the basal parts and axillary buds revealed an abundance of related biological processes underlying the axillary bud growth of plants under N treatments. Among these processes, the activity of cell division and expansion was positively correlated with the growth rate of axillary buds of plants grown under different N supplies. Additionally, TFs and phytohormones were shown to play crucial roles in determining the axillary bud growth of plants grown under different N concentrations. Further validation of OsGS1;2 and OsGS2 , the rice mutants of which presented altered tiller numbers, validated our transcriptomic data. Conclusion: These results indicate that different N concentrations affect the axillary bud growth rate, and our study revealed comprehensive expression profiles of genes that respond to different N concentrations, providing an important resource for future studies attempting to determine how axillary bud growth is controlled by different N supplies.

scholarly journals Transcriptional terminators allow leak-free chromosomal integration of genetic constructs in cyanobacteria

2019 ◽  
Author(s):  
Ciarán L. Kelly ◽  
George M. Taylor ◽  
Aiste Satkute ◽  
Linda Dekker ◽  
John T. Heap
Keyword(s):  
Gene Expression ◽  
Expression System ◽  
Chromosomal Integration ◽  
Rna Structures ◽  
Independent Control ◽  
Poor Control ◽  
Control Of Gene Expression ◽  
Genomic Locations ◽  
Genetic Constructs ◽  
Control Of Expression

AbstractCyanobacteria are promising candidates for sustainable bioproduction of chemicals from sunlight and carbon dioxide. However, the genetics and metabolism of cyanobacteria are less well understood than model heterotrophic organisms, and the suite of well characterised cyanobacterial genetic tools and parts is less mature and complete. Transcriptional terminators use specific RNA structures to halt transcription and are routinely used in both natural and recombinant contexts to achieve independent control of gene expression and ‘insulate’ genes and operons from one another. Insulating gene expression can be particularly important when heterologous/synthetic genetic constructs are inserted at genomic locations where transcriptional read-through from chromosomal promoters occurs, resulting in poor control of expression of the introduced genes. To date, few terminators have been described and characterised in cyanobacteria. In this work, nineteen heterologous, synthetic or putative native Rho-independent (intrinsic) terminators were tested in the model freshwater cyanobacterium,Synechocystissp. PCC 6803, from which eleven strong terminators were identified. A subset of these strong terminators was then used to successfully insulate a chromosomally-integrated rhamnose-induciblerhaBADexpression system from hypothesised ‘read-through’ from a neighbouring chromosomal promoter, resulting in greatly improved inducible control. The addition of validated strong terminators to the cyanobacterial toolkit will allow improved independent control of introduced genes.

The hormonal regulation of axillary bud growth in Arabidopsis

2000 ◽  
Vol 24 (2) ◽  
pp. 159-169 ◽  
Author(s):  
Steven P. Chatfield ◽  
Petra Stirnberg ◽  
Brian G. Forde ◽  
Ottoline Leyser
Keyword(s):  
Hormonal Regulation ◽  
Axillary Bud ◽  
Bud Growth ◽  
Axillary Bud Growth
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