scholarly journals A proof of concept study for the differentiation of SARS-CoV-2, hCoV-NL63, and IAV-H1N1 in vitro cultures using ion mobility spectrometry

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
M. Feuerherd ◽  
A.-K. Sippel ◽  
J. Erber ◽  
J. I. Baumbach ◽  
R. M. Schmid ◽  
...  
Keyword(s):  
Organic Compounds ◽  
Ion Mobility ◽  
Point Of Care ◽  
Respiratory Viruses ◽  
Gas Analysis ◽  
In Vitro Cultures ◽  
Proof Of Concept ◽  
Volatile Organic ◽  
Set Up

AbstractRapid, high-throughput diagnostic tests are essential to decelerate the spread of the novel coronavirus disease 2019 (COVID-19) pandemic. While RT-PCR tests performed in centralized laboratories remain the gold standard, rapid point-of-care antigen tests might provide faster results. However, they are associated with markedly reduced sensitivity. Bedside breath gas analysis of volatile organic compounds detected by ion mobility spectrometry (IMS) may enable a quick and sensitive point-of-care testing alternative. In this proof-of-concept study, we investigated whether gas analysis by IMS can discriminate severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) from other respiratory viruses in an experimental set-up. Repeated gas analyses of air samples collected from the headspace of virus-infected in vitro cultures were performed for 5 days. A three-step decision tree using the intensities of four spectrometry peaks correlating to unidentified volatile organic compounds allowed the correct classification of SARS-CoV-2, human coronavirus-NL63, and influenza A virus H1N1 without misassignment when the calculation was performed with data 3 days post infection. The forward selection assignment model allowed the identification of SARS-CoV-2 with high sensitivity and specificity, with only one of 231 measurements (0.43%) being misclassified. Thus, volatile organic compound analysis by IMS allows highly accurate differentiation of SARS-CoV-2 from other respiratory viruses in an experimental set-up, supporting further research and evaluation in clinical studies.

scholarly journals Trichoderma asperellum T76-14 Released Volatile Organic Compounds against Postharvest Fruit Rot in Muskmelons (Cucumis melo) Caused by Fusarium incarnatum

2021 ◽  
Vol 7 (1) ◽  
pp. 46
Author(s):  
Warin Intana ◽  
Suchawadee Kheawleng ◽  
Anurag Sunpapao
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Cucumis Melo ◽  
Fungal Growth ◽  
Fruit Rot ◽  
Postharvest Disease ◽  
Dual Culture ◽  
Trichoderma Asperellum ◽  
Volatile Organic

Postharvest fruit rot caused by Fusarium incarnatum is a destructive postharvest disease of muskmelon (Cucumis melo). Biocontrol by antagonistic microorganisms is considered an alternative to synthetic fungicide application. The aim of this study was to investigate the mechanisms of action involved in the biocontrol of postharvest fruit rot in muskmelons by Trichoderma species. Seven Trichoderma spp. isolates were selected for in vitro testing against F. incarnatum in potato dextrose agar (PDA) by dual culture assay. In other relevant works, Trichoderma asperellum T76-14 showed a significantly higher percentage of inhibition (81%) than other isolates. Through the sealed plate method, volatile organic compounds (VOCs) emitted from T. asperellum T76-14 proved effective at inhibiting the fungal growth of F. incarnatum by 62.5%. Solid-phase microextraction GC/MS analysis revealed several VOCs emitted from T. asperellum T76-14, whereas the dominant compound was tentatively identified as phenylethyl alcohol (PEA). We have tested commercial volatile (PEA) against in vitro growth of F. incarnatum; the result showed PEA at a concentration of 1.5 mg mL−1 suppressed fungal growth with 56% inhibition. Both VOCs and PEA caused abnormal changes in the fungal mycelia. In vivo testing showed that the lesion size of muskmelons exposed to VOCs from T. asperellum T76-14 was significantly smaller than that of the control. Muskmelons exposed to VOCs from T. asperellum T76-14 showed no fruit rot after incubation at seven days compared to fruit rot in the control. This study demonstrated the ability of T. asperellum T76-14 to produce volatile antifungal compounds, showing that it can be a major mechanism involved in and responsible for the successful inhibition of F. incarnatum and control of postharvest fruit rot in muskmelons.

scholarly journals Proof of concept study: Testing human volatile organic compounds as tools for age classification of films

PLoS ONE ◽  
2018 ◽  
Vol 13 (10) ◽  
pp. e0203044 ◽  
Author(s):  
C. Stönner ◽  
A. Edtbauer ◽  
B. Derstroff ◽  
E. Bourtsoukidis ◽  
T. Klüpfel ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Proof Of Concept ◽  
Concept Study ◽  
Volatile Organic

scholarly journals In vitro profiling of volatile organic compounds released by Simpson-Golabi-Behmel syndrome adipocytes

2019 ◽  
Vol 1104 ◽  
pp. 256-261 ◽  
Author(s):  
Paweł Mochalski ◽  
Eva Diem ◽  
Karl Unterkofler ◽  
Axel Mündlein ◽  
Heinz Drexel ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Volatile Organic

Volatile Organic Compounds in Human Breath: Biogenic Origin and Point-of-Care Analysis Approaches

2013 ◽  
pp. 129-154 ◽  
Author(s):  
Alexander A. Aksenov ◽  
Michael Schivo ◽  
Hamzeh Bardaweel ◽  
Yuriy Zrodnikov ◽  
Alice M. Kwan ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Point Of Care ◽  
Human Breath ◽  
Biogenic Origin ◽  
Volatile Organic

scholarly journals Bioactivity of volatile organic compounds by Aureobasidium species against gray mold of tomato and table grape

2020 ◽  
Vol 36 (11) ◽  
Author(s):  
A. Di Francesco ◽  
J. Zajc ◽  
N. Gunde-Cimerman ◽  
E. Aprea ◽  
F. Gasperi ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Mycelial Growth ◽  
Solid Phase ◽  
Table Grape ◽  
Conidial Suspension ◽  
Vitro Assay ◽  
Volatile Organic

Abstract Aureobasidium strains isolated from diverse unconventional environments belonging to the species A. pullulans, A. melanogenum, and A. subglaciale were evaluated for Volatile Organic Compounds (VOCs) production as a part of their modes of action against Botrytis cinerea of tomato and table grape. By in vitro assay, VOCs generated by the antagonists belonging to the species A. subglaciale showed the highest inhibition percentage of the pathogen mycelial growth (65.4%). In vivo tests were conducted with tomatoes and grapes artificially inoculated with B. cinerea conidial suspension, and exposed to VOCs emitted by the most efficient antagonists of each species (AP1, AM10, AS14) showing that VOCs of AP1 (A. pullulans) reduced the incidence by 67%, partially confirmed by the in vitro results. Conversely, on table grape, VOCs produced by all the strains did not control the fungal incidence but were only reducing the infection severity (< 44.4% by A. pullulans; < 30.5% by A. melanogenum, and A. subglaciale). Solid-phase microextraction (SPME) and subsequent gas chromatography coupled to mass spectrometry identified ethanol, 3-methyl-1-butanol, 2-methyl-1-propanol as the most produced VOCs. However, there were differences in the amounts of produced VOCs as well as in their repertoire. The EC50 values of VOCs for reduction of mycelial growth of B. cinerea uncovered 3-methyl-1-butanol as the most effective compound. The study demonstrated that the production and the efficacy of VOCs by Aureobasidium could be directly related to the specific species and pathosystem and uncovers new possibilities for searching more efficient VOCs producing strains in unconventional habitats other than plants.

In vitro profiling of endothelial volatile organic compounds under resting and pro-inflammatory conditions

Metabolomics ◽  
2019 ◽  
Vol 15 (10) ◽  
Author(s):  
V. Longo ◽  
A. Forleo ◽  
S. Capone ◽  
E. Scoditti ◽  
M. A. Carluccio ◽  
...  
Keyword(s):  
Volatile Organic Compounds ◽  
Organic Compounds ◽  
Inflammatory Conditions ◽  
Volatile Organic
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