Rats exhibit age-related mosaic loss of chromosome Y

AbstractMosaic loss of the Y chromosome (LOY) is the most frequent chromosomal aberration in aging men and is strongly correlated with mortality and disease. To date, studies of LOY have only been performed in humans, and so it is unclear whether LOY is a natural consequence of our relatively long lifespan or due to exposure to human-specific external stressors. Here, we explored whether LOY could be detected in rats. We applied a locus-specific PCR and target sequencing approach that we used as a proxy to estimate LOY in 339 samples covering eleven tissues from young and old individuals. We detected LOY in four tissues of older rats. To confirm the results from the PCR screening, we re-sequenced 60 full genomes from old rats, which revealed that the Y chromosome is the sole chromosome with low copy numbers. Finally, our results suggest that LOY is associated with other structural aberrations on the Y chromosome and possibly linked to the mosaic loss of the X chromosome. This is the first report, to our knowledge, demonstrating that the patterns of LOY observed in aging men are also present in a rodent, and conclude that LOY may be a natural process in placental mammals.

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Age-Related Attenuation of Aerobic Exercise Training Adaptation in 24-Week Old Rats

Diabetes ◽

10.2337/db18-1916-p ◽

2018 ◽

Vol 67 (Supplement 1) ◽

pp. 1916-P

Author(s):

REBECCA L. SCALZO ◽

GRAHAME F. EVANS ◽

SARA E. HULL ◽

LESLIE KNAUB ◽

LORI A. WALKER ◽

...

Keyword(s):

Exercise Training ◽

Aerobic Exercise ◽

Aerobic Exercise Training ◽

Training Adaptation ◽

Age Related ◽

Old Rats

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Effects of trenbolone acetate and testosterone on growth and on plasma concentrations of corticosterone and ACTH in rats

Journal of Endocrinology ◽

10.1677/joe.0.1260461 ◽

1990 ◽

Vol 126 (3) ◽

pp. 461-466 ◽

Cited By ~ 8

Author(s):

M. N. Sillence ◽

R. G. Rodway

Keyword(s):

Weight Gain ◽

Growth Response ◽

Plasma Concentrations ◽

Female Rats ◽

Male Rats ◽

Adrenal Weight ◽

Trenbolone Acetate ◽

Male And Female Rats ◽

Age Related ◽

Old Rats

ABSTRACT The effects of trenbolone acetate (TBA) on growth and on plasma concentrations of corticosterone were examined in male and female rats. At 5 weeks of age, rats were injected with TBA (0·8 mg/kg) dissolved in peanut oil, or with oil alone, daily for 10 days. In female rats, TBA caused an increase in weight gain (20–38%), a reduction in adrenal weight (19%) and a reduction in plasma concentrations of corticosterone (55%). In contrast, TBA-treated male rats showed no significant increase in weight gain, no significant change in adrenal weight and no reduction in plasma concentrations of corticosterone. The mechanism by which adrenal activity was suppressed in TBA-treated female rats was examined and the response compared with that to testosterone. Female rats (8 weeks old) were injected daily either with oil vehicle, TBA (0·8 mg/kg) or testosterone propionate (0·8 mg/kg). Testosterone increased weight gain (24%), but the growth response to TBA treatment was significantly greater (97%). A reduction in plasma concentrations of corticosterone (45%) was again observed in response to TBA. However, testosterone increased plasma concentrations of corticosterone (52%) above those of control values. Neither androgen affected plasma concentrations of ACTH. Finally, the effects of TBA were examined in 6-week-old female rats, to characterize further the apparent age-related increase in responsiveness. The growth response of 6-week-old rats (60–74%) was intermediate between that seen in 5- and 8-week-old animals. It is concluded that part of the anabolic activity of TBA may be related to a reduction in circulating concentrations of corticosterone. The effect of TBA on corticosterone concentrations differs from that of the natural androgen, testosterone, and does not appear to be mediated by a reduction in plasma concentrations of ACTH. Journal of Endocrinology (1990) 126, 461–466

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Modulation of Age-Related Biochemical Changes and Oxidative Stress by Vitamin C and Glutathione Supplementation in Old Rats

Annals of Nutrition and Metabolism ◽

10.1159/000065402 ◽

2002 ◽

Vol 46 (5) ◽

pp. 165-168 ◽

Cited By ~ 11

Author(s):

M.A. Amer

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Biochemical Changes ◽

Age Related ◽

Old Rats ◽

And Oxidative Stress

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The virome of German bats: comparing virus discovery approaches

Scientific Reports ◽

10.1038/s41598-021-86435-4 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Claudia Kohl ◽

Annika Brinkmann ◽

Aleksandar Radonić ◽

Piotr Wojtek Dabrowski ◽

Kristin Mühldorfer ◽

...

Keyword(s):

Virus Detection ◽

Detection Methods ◽

Single Type ◽

Metagenomic Sequencing ◽

Virus Discovery ◽

Highly Pathogenic ◽

Pcr Screening ◽

Specific Pcr ◽

Pathogenic Viruses ◽

Viral Sequences

AbstractBats are known to be reservoirs of several highly pathogenic viruses. Hence, the interest in bat virus discovery has been increasing rapidly over the last decade. So far, most studies have focused on a single type of virus detection method, either PCR, virus isolation or virome sequencing. Here we present a comprehensive approach in virus discovery, using all three discovery methods on samples from the same bats. By family-specific PCR screening we found sequences of paramyxoviruses, adenoviruses, herpesviruses and one coronavirus. By cell culture we isolated a novel bat adenovirus and bat orthoreovirus. Virome sequencing revealed viral sequences of ten different virus families and orders: three bat nairoviruses, three phenuiviruses, one orbivirus, one rotavirus, one orthoreovirus, one mononegavirus, five parvoviruses, seven picornaviruses, three retroviruses, one totivirus and two thymoviruses were discovered. Of all viruses identified by family-specific PCR in the original samples, none was found by metagenomic sequencing. Vice versa, none of the viruses found by the metagenomic virome approach was detected by family-specific PCRs targeting the same family. The discrepancy of detected viruses by different detection approaches suggests that a combined approach using different detection methods is necessary for virus discovery studies.

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Loss of the Y Chromosome: An Age-Related or Clonal Phenomenon in Acute Myelogenous Leukemia/Myelodysplastic Syndrome?

Archives of Pathology & Laboratory Medicine ◽

10.5858/2008-132-1329-lotyca ◽

2008 ◽

Vol 132 (8) ◽

pp. 1329-1332

Author(s):

Anna K. Wong ◽

Belle Fang ◽

Ling Zhang ◽

Xiuqing Guo ◽

Stephen Lee ◽

...

Keyword(s):

Bone Marrow ◽

Myelodysplastic Syndrome ◽

Y Chromosome ◽

Acute Myelogenous Leukemia ◽

Myelogenous Leukemia ◽

Related Phenomenon ◽

Patients Âgés ◽

Age Related ◽

Acute Myelogenous ◽

Bone Marrow Analysis

Abstract Context.—The clinical association between loss of the Y chromosome and acute myelogenous leukemia and myelodysplastic syndrome (AML/MDS) has been debated because both phenomena are related to aging. A prior publication suggests that loss of the Y chromosome in more than 75% of cells may indicate a clonal phenomenon that could be a marker for hematologic disease. Objective.—To evaluate the relationship between loss of the Y chromosome and AML/MDS. Design.—A retrospective review of cytogenetic reports of 2896 male patients ascertained from 1996 to 2007 was performed. Results were stratified based on the percentage of cells missing the Y chromosome and were correlated with patients' ages and bone marrow biopsy reports through logistic regression analysis with adjustment for age. Results.—Loss of the Y chromosome was found in 142 patients. Of these, 16 patients demonstrated myeloid disease, with 2 cases of AML and 14 cases of MDS. An increased incidence (P < .05) of AML/MDS was seen only in the group composed of 8 patients with complete loss of the Y chromosome in all karyotyped cells (1 case of AML and 7 cases of MDS). Conclusion.—Loss of the Y chromosome appears to be primarily an age-related phenomenon. However, in individuals in which all cells on cytogenetic analysis showed loss of the Y chromosome, there was a statistically significant increase in AML/MDS, suggesting that the absence of any normal-dividing cells in a bone marrow analysis may be indicative of AML/MDS.

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Pituitary-adrenal response to bacterial endotoxin in developing rats

AJP Endocrinology and Metabolism ◽

10.1152/ajpendo.1988.255.4.e525 ◽

1988 ◽

Vol 255 (4) ◽

pp. E525-E530 ◽

Cited By ~ 8

Author(s):

L. Witek-Janusek

Keyword(s):

Plasma Corticosterone ◽

Neonatal Rat ◽

Bacterial Endotoxin ◽

Plasma Acth ◽

Adult Rats ◽

Age Related ◽

Corticosterone Response ◽

Adrenal Response ◽

Old Rats ◽

Related Pattern

The neonatal rat is very sensitive to the lethal effects of bacterial endotoxin. Because of the adaptive importance of pituitary-adrenal secretions to stress, this study examined the ontogeny of the plasma corticosterone and adrenocorticotropic hormone (ACTH) responses to endotoxin. The lethal sensitivity of young rats to endotoxin ranged from 0.5 to 30 mg/kg (ip) in the 1- to 21-day-old rat. After endotoxin treatment, the 1- and 2-day-old rat showed marked elevations of corticosterone similar in magnitude to that seen in 21-day-old and adult rats; however, significantly depressed corticosterone increments were observed in the 5-, 10-, and 14-day-old rats. This age-related pattern of adrenocortical secretion was correlated with the developing rat's corticosterone response to exogenous ACTH. In contrast, endotoxin administered to 5-, 10-, and 14-day-old rats resulted in increments of plasma ACTH similar to those observed in the 21-day-old and adult rats. Although plasma ACTH levels increased by 84-127% in the 1- and 2-day-old rats, these increases were significantly less than those of rats at all other ages tested. Thus the newborn rat mounts an effective corticosterone response to endotoxin, loses this ability between ages 5-14 days, and regains this response at 21 days of age. Because the hyporesponsive ages exhibit a marked increase in ACTH secretion, the loss of the adrenocortical response to endotoxin appears to be a result of a depressed responsiveness of the adrenal cortex to ACTH.

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Acetyl-l-Carnitine Supplementation to Old Rats Partially Reverts the Age-Related Mitochondrial Decay of Soleus Muscle by Activating Peroxisome Proliferator-Activated Receptor γ Coactivator-1α–Dependent Mitochondrial Biogenesis

Rejuvenation Research ◽

10.1089/rej.2009.0955 ◽

2010 ◽

Vol 13 (2-3) ◽

pp. 148-151 ◽

Cited By ~ 19

Author(s):

Vito Pesce ◽

Flavio Fracasso ◽

Pierluigi Cassano ◽

Angela Maria Serena Lezza ◽

Palmiro Cantatore ◽

...

Keyword(s):

Soleus Muscle ◽

Mitochondrial Biogenesis ◽

Peroxisome Proliferator ◽

Carnitine Supplementation ◽

Peroxisome Proliferator Activated Receptor ◽

Age Related ◽

Old Rats

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Age-related changes in endothelial permeability and distribution volume of albumin in rat aorta

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1993.264.3.h679 ◽

1993 ◽

Vol 264 (3) ◽

pp. H679-H685 ◽

Cited By ~ 7

Author(s):

J. Belmin ◽

B. Corman ◽

R. Merval ◽

A. Tedgui

Keyword(s):

Arterial Wall ◽

Relative Concentration ◽

Endothelial Permeability ◽

Distribution Volume ◽

Albumin Concentration ◽

Macromolecular Transport ◽

Age Related ◽

The Media ◽

Old Rats ◽

Age Related Changes

Age-related changes in macromolecular transport across the arterial wall were investigated in 10-, 20-, and 30-mo-old WAG/Rij rats. Animals were injected with 125I- and 131I-labeled albumin, 90 and 5 min before they were killed, respectively. The transmural distribution of relative concentration of tracers in the aortic wall was obtained using en face serial sectioning technique. The apparent endothelial permeability to albumin calculated from the distribution of 5-min 131I-labeled albumin concentrations was significantly enhanced in 20- and 30-mo-old rats compared with 10-mo-old rats. The apparent distribution volume of albumin within the media, estimated as the mean medial 125I-labeled albumin concentration, was not significantly changed in 20-mo-old rats but was significantly decreased in the 30-mo-old animals. These age-related changes in the macromolecular transport suggest that the entry of plasma macromolecules in the aged arterial wall might be enhanced, whereas the efflux through the media may be impeded, possibly contributing to their trapping in the subendothelium.

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Are skeletally mature female rats a suitable model to study osteoporosis?

Arquivos Brasileiros de Endocrinologia & Metabologia ◽

10.1590/s0004-27302012000400007 ◽

2012 ◽

Vol 56 (4) ◽

pp. 259-264 ◽

Cited By ~ 4

Author(s):

Claudia Cardoso Netto ◽

Vivian Cristine Correia Vieira ◽

Lizanka Paola Figueiredo Marinheiro ◽

Sherry Agellon ◽

Hope Weiler ◽

...

Keyword(s):

Bone Metabolism ◽

Type I Collagen ◽

Biomechanical Properties ◽

Mature Female ◽

Female Rats ◽

Suitable Model ◽

Type I ◽

Age Related ◽

Female Wistar Rats ◽

Old Rats

OBJECTIVE: To analyze if female Wistar rats at 56 weeks of age are a suitable model to study osteoporosis. MATERIALS AND METHODS: Female rats with 6 and 36 weeks of age (n = 8 per group) were kept over a 20-week period and fed a diet for mature rodents complete in terms of Ca, phosphorous, and vitamin D. Excised femurs were measured for bone mass using dual-energy x-ray absorptiometry, morphometry, and biomechanical properties. The following serum mar-kers of bone metabolism were analyzed: parathyroid hormone (PTH), osteocalcin (OC), osteoprotegerin (OPG), receptor activator of nuclear factor Κappa B ligand (RANKL), C-terminal peptides of type I collagen (CTX-I), total calcium, and alkaline phosphatase (ALP) activity. RESULTS: Rats at 56 weeks of age showed important bone metabolism differences when compared with the younger group, such as, highest diaphysis energy to failure, lowest levels of OC, CTX-I, and ALP, and elevated PTH, even with adequate dietary Ca. CONCLUSION: Rats at 26-week-old rats may be too young to study age-related bone loss, whereas the 56-week-old rats may be good models to represent the early stages of age-related changes in bone metabolism.

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