scholarly journals Possible Involvement of F1F0-ATP synthase and Intracellular ATP in Keratinocyte Differentiation in normal skin and skin lesions

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Xie Xiaoyun ◽  
Han Chaofei ◽  
Zeng Weiqi ◽  
Chen Chen ◽  
Lu Lixia ◽  
...  
Keyword(s):  
Atp Synthase ◽  
Normal Skin ◽  
Skin Lesions ◽  
Keratinocyte Differentiation ◽  
Intracellular Atp ◽  
F1f0 Atp Synthase

scholarly journals miR-155 Contributes to Normal Keratinocyte Differentiation and Is Upregulated in the Epidermis of Psoriatic Skin Lesions

2020 ◽  
Vol 21 (23) ◽  
pp. 9288
Author(s):  
Lucian Beer ◽  
Polina Kalinina ◽  
Martin Köcher ◽  
Maria Laggner ◽  
Markus Jeitler ◽  
...  
Keyword(s):  
Skin Diseases ◽  
Normal Skin ◽  
Skin Lesions ◽  
Keratinocyte Differentiation ◽  
Psoriatic Skin ◽  
Bioinformatics Analyses ◽  
Epidermal Barrier ◽  
Barrier Formation

The role of microRNAs (miRNAs) during keratinocyte (KC) differentiation and in skin diseases with epidermal phenotypes has attracted strong interest over the past few years. However, combined mRNA and miRNA expression analyses to elucidate the intricate mRNA–miRNA networks of KCs at different stages of differentiation have not been performed yet. In the present study, we investigated the dynamics of miRNA and mRNA expression during KC differentiation in vitro and in normal and psoriatic epidermis. While we identified comparable numbers of up- and downregulated mRNAs (49% and 51%, respectively), miRNAs were predominantly upregulated (76% vs 24%) during KC differentiation. Further bioinformatics analyses suggested an important inhibitory role for miR-155 in KC differentiation, as it was repressed during KC differentiation in normal skin but strongly upregulated in the epidermis of psoriatic skin lesions. Mimicking the inflammatory milieu of psoriatic skin in vitro, we could show that the pro-inflammatory cytokines IL17, IL1β and INFγ synergistically upregulated miR-155 expression in KCs. Forced over-expression of miR-155 in human in vitro skin models specifically reduced the expression of loricrin (LOR) in KCs, indicating that miR-155 interferes with the establishment of a normal epidermal barrier. Together, our data indicate that downregulation of miR-155 during KC differentiation is a crucial step for epidermal barrier formation. Furthermore, its strong upregulation in psoriatic lesions suggests a contributing role of miR-155 in the altered keratinocyte differentiation observed in psoriasis. Therefore, miR-155 represents as a potential target for treating psoriatic skin lesions.

scholarly journals Inhibition of Hippo Signaling Improves Skin Lesions in a Rosacea-Like Mouse Model

2021 ◽  
Vol 22 (2) ◽  
pp. 931
Author(s):  
Jihyun Lee ◽  
Yujin Jung ◽  
Seo won Jeong ◽  
Ga Hee Jeong ◽  
Gue Tae Moon ◽  
...  
Keyword(s):  
Mouse Model ◽  
Signaling Pathway ◽  
Normal Skin ◽  
Skin Lesions ◽  
Hippo Signaling ◽  
Vascular Endothelial ◽  
Expression Levels ◽  
Hippo Signaling Pathway ◽  
Endothelial Growth Factor

The Hippo signaling pathway plays a key role in regulating organ size and tissue homeostasis. Hippo and two of its main effectors, yes-associated protein (YAP) and WWTR1 (WW domain-containing transcription regulator 1, commonly listed as TAZ), play critical roles in angiogenesis. This study investigated the role of the Hippo signaling pathway in the pathogenesis of rosacea. We performed immunohistochemical analyses to compare the expression levels of YAP and TAZ between rosacea skin and normal skin in humans. Furthermore, we used a rosacea-like BALB/c mouse model induced by LL-37 injections to determine the roles of YAP and TAZ in rosacea in vivo. We found that the expression levels of YAP and TAZ were upregulated in patients with rosacea. In the rosacea-like mouse model, we observed that the clinical features of rosacea, including telangiectasia and erythema, improved after the injection of a YAP/TAZ inhibitor. Additionally, treatment with a YAP/TAZ inhibitor reduced the expression levels of YAP and TAZ and diminished vascular endothelial growth factor (VEGF) immunoreactivity in the rosacea-like mouse model. Our findings suggest that YAP/TAZ inhibitors can attenuate angiogenesis associated with the pathogenesis of rosacea and that both YAP and TAZ are potential therapeutic targets for patients with rosacea.

scholarly journals Mutagenesis of subunit delta from Escherichia coli F1F0-ATP synthase.

1994 ◽  
Vol 269 (1) ◽  
pp. 418-426
Author(s):  
A.L. Hazard ◽  
A.E. Senior
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
F1f0 Atp Synthase

scholarly journals Insertion scanning mutagenesis of subunit a of the F1F0 ATP synthase near His245and implications on gating of the proton channel.

1998 ◽  
Vol 273 (34) ◽  
pp. 22159
Author(s):  
Steven B. Vik ◽  
Annette Rochelle Patterson ◽  
Barbara J. Antonio
Keyword(s):  
Atp Synthase ◽  
Proton Channel ◽  
Subunit A ◽  
F1f0 Atp Synthase

scholarly journals Molecular cloning and characterization of cDNAs for the gamma- and epsilon-subunits of mitochondrial F1F0 ATP synthase from the sweet potato

1993 ◽  
Vol 268 (23) ◽  
pp. 17205-17210
Author(s):  
A. Morikami ◽  
G. Ehara ◽  
K. Yuuki ◽  
K. Nakamura
Keyword(s):  
Molecular Cloning ◽  
Sweet Potato ◽  
Atp Synthase ◽  
F1f0 Atp Synthase

scholarly journals Defective energy coupling in delta-subunit mutants of Escherichia coli F1F0-ATP synthase.

1994 ◽  
Vol 269 (1) ◽  
pp. 427-432 ◽  
Author(s):  
A.L. Hazard ◽  
A.E. Senior
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
Energy Coupling ◽  
F1f0 Atp Synthase ◽  
Delta Subunit

scholarly journals Mutations in the delta subunit influence the assembly of F1F0 ATP synthase in Escherichia coli.

1994 ◽  
Vol 176 (2) ◽  
pp. 540-542 ◽  
Author(s):  
A E Stack ◽  
B D Cain
Keyword(s):  
Escherichia Coli ◽  
Atp Synthase ◽  
F1f0 Atp Synthase ◽  
Delta Subunit
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