Phosphorylation and dephosphorylation of polyhydroxy compounds by class A bacterial acid phosphatases

2003 ◽  
Vol 1 (16) ◽  
pp. 2833 ◽  
Author(s):  
Naoko Tanaka ◽  
Zulfiqar Hasan ◽  
Aloysius F. Hartog ◽  
Teunie van Herk ◽  
Ron Wever
Keyword(s):  
Acid Phosphatases ◽  
Polyhydroxy Compounds ◽  
Class A

scholarly journals Isolation, Cloning, and Expression of an Acid Phosphatase Containing Phosphotyrosyl Phosphatase Activity fromPrevotella intermedia

1999 ◽  
Vol 181 (22) ◽  
pp. 7107-7114 ◽  
Author(s):  
Xiaochi Chen ◽  
Toshihiro Ansai ◽  
Shuji Awano ◽  
Toshiya Iida ◽  
Sailen Barik ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Amino Acid ◽  
Growth Factor Receptor ◽  
Amino Acid Sequences ◽  
Cloning And Expression ◽  
Striking Similarity ◽  
Sequence Motif ◽  
Acid Phosphatases ◽  
Class A ◽  
Lipid Phosphatases

ABSTRACT A novel acid phosphatase containing phosphotyrosyl phosphatase (PTPase) activity, designated PiACP, from Prevotella intermedia ATCC 25611, an anaerobe implicated in progressive periodontal disease, has been purified and characterized. PiACP, a monomer with an apparent molecular mass of 30 kDa, did not require divalent metal cations for activity and was sensitive to orthovanadate but highly resistant to okadaic acid. The enzyme exhibited substantial activity against tyrosine phosphate-containing peptides derived from the epidermal growth factor receptor. On the basis of N-terminal and internal amino acid sequences of purified PiACP, the gene coding for PiACP was isolated and sequenced. The PiACP gene consisted of 792 bp and coded for a basic protein with an M r of 29,164. The deduced amino acid sequence exhibited striking similarity (25 to 64%) to those of members of class A bacterial acid phosphatases, including PhoC of Morganella morganii, and involved a conserved phosphatase sequence motif that is shared among several lipid phosphatases and the mammalian glucose-6-phosphatases. The highly conservative motif HCXAGXXR in the active domain of PTPase was not found in PiACP. Mutagenesis of recombinant PiACP showed that His-170 and His-209 were essential for activity. Thus, the class A bacterial acid phosphatases including PiACP may function as atypical PTPases, the biological functions of which remain to be determined.

Localization of acid phosphatases in root cap of rice plant

1991 ◽  
Vol 49 ◽  
pp. 224-225
Author(s):  
Y. R. Chen ◽  
Y. F. Huang ◽  
W. S. Chen
Keyword(s):  
Rice Plant ◽  
Developmental Stages ◽  
Uranyl Acetate ◽  
Root Cap ◽  
Plant Tissues ◽  
Acid Phosphatases ◽  
Root Tips ◽  
Buffer Solution ◽  
Cacodylate Buffer ◽  
Ethanol Series

Acid phosphatases are widely distributed in different tisssues of various plants. Studies on subcellular localization of acid phosphatases show they might be present in cell wall, plasma lemma, mitochondria, plastid, vacuole and nucleus. However, their localization in rice cell varies with developmental stages of cells and plant tissues. In present study, acid phosphatases occurring in root cap are examined.Sliced root tips of ten-day-old rice(Oryza sativa) seedlings were fixed in 0.1M cacodylate buffer containing 2.5% glutaraldehyde for 2h, washed overnight in same buffer solution, incubated in Gomori's solution at 37° C for 90min, post-fixed in OsO4, dehydrated in ethanol series and finally embeded in Spurr's resin. Sections were doubly stained with uranyl acetate and lead citrate, and observed under Hitachi H-600 at 75 KV.

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

The role of acid phosphatases in plant phosphorus metabolism

1994 ◽  
Vol 90 (4) ◽  
pp. 791-800 ◽  
Author(s):  
Stephen M. G. Duff ◽  
Gautam Sarath ◽  
William C. Plaxton
Keyword(s):  
Phosphorus Metabolism ◽  
Acid Phosphatases

Important Compounds in the Cocaine Class: A Synthesis Overview

1991 ◽  
Author(s):  
F.I. Carroll ◽  
◽  
Anita H. Lewin
Keyword(s):  
Class A

DISTRIBUIÇÃO DA EVAPORAÇÃO EM ESTUFA PLÁSTICA NA PRIMAVERA

Irriga ◽  
2001 ◽  
Vol 6 (3) ◽  
pp. 120-127
Author(s):  
Reginaldo Ferreira Santos ◽  
Antonio Evaldo Klar
Keyword(s):  
Spatial Variability ◽  
Pan Evaporation ◽  
Spring Season ◽  
Class A ◽  
Agricultural Sciences ◽  
E Mail ◽  
Center Area ◽  
Class A Pan

DISTRIBUIÇÃO DA EVAPORAÇÃO EM ESTUFA PLÁSTICA NA PRIMAVERA  Reginaldo Ferreira SantosCentro de Ciências Exatas e Tecnológica da UNIOESTE- CP 711CEP 858114-110, Cascavel, PR - Fone: 0XX45 2203155.  E-mail: [email protected] Evaldo KlarDepartamento de Engenharia Rural - Faculdade de Ciências Agronômica- UNESP - CEP 18603-970 - Botucatu, SP. CP: 237.  E-mail:  [email protected]  1  RESUMO O presente trabalho teve como objetivo avaliar a distribuição da evaporação no interior de uma estufa plástica, com uma cultura de pimentão, através da variabilidade espacial e comparar a evaporação dos microevaporímetros com os valores do Tanque classe "A". O experimento foi conduzido no Campus da Universidade Estadual Paulista - FCA/UNESP, no período de primavera, em estufa plástica de polietileno de baixa densidade (PEBD). Na distribuição da evaporação em estufa com orientação norte/sul, verificou-se que as maiores evaporações ocorreram nas extremidades sul e norte tendente ao lado oeste. Já as menores evaporações localizaram-se no centro. No período de primavera, a evaporação média nos microevaporímetros superestimou em 55% a evaporação determinada no Tanque classe "A". UNITERMOS: evaporação, geoestatística, estufa.  SANTOS, R.F, KLAR, A.E.  EVAPORATION DISTRIBUTION INSIDE A PLASTIC TUNNEL IN THE SPRING SEASON  2  ABSTRACT                 The main aim of this study was to verify the evaporation distribution inside a plastic tunnel, with pepper crop, oriented to north/south, through spatial variability and to compare Class A Pan evaporation to punctual evaporations of 40 equidistant microevaporimeters placed from 50cm the soil. The study was carried out at the College of Agricultural Sciences/UNESP, Botucatu – SP in the spring season.  The highest evaporation occurred next to north and to south sides of the tunnel, with tendency to west. Consequently, the lowest evaporations occurred at the center area. The microevaporimeter evaporations were 55% higher than those obtained from Class A Pan. KEYWORDS: evaporation distribution, microevaporimeter.

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