A graphene oxide-based AIE biosensor with high selectivity toward bovine serum albumin

2011 ◽  
Vol 47 (45) ◽  
pp. 12385 ◽  
Author(s):  
Xiujuan Xu ◽  
Jing Huang ◽  
Jijun Li ◽  
Jiawei Yan ◽  
Jingui Qin ◽  
...  
Author(s):  
Kunal Biswas ◽  
Avik Sett ◽  
Monojit Mondal ◽  
Srijeet Tripathy ◽  
Jaya Bandyopadhyay ◽  
...  

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 792
Author(s):  
Febri Baskoro ◽  
Selvaraj Rajesh Kumar ◽  
Shingjiang Jessie Lue

This study investigates the permeance and rejection efficiencies of different dyes (Rhodamine B and methyl orange), folic acid and a protein (bovine serum albumin) using graphene oxide composite membrane. The ultrathin separation layer of graphene oxide (thickness of 380 nm) was successfully deposited onto porous polyvinylidene fluoride-polyacrylic acid intermediate layer on nonwoven support layer using vacuum filtration. The graphene oxide addition in the composite membrane caused an increased hydrophilicity and negative surface charge than those of the membrane without graphene oxide. In the filtration process using a graphene oxide composite membrane, the permeance values of pure water, dyes, folic acid and bovine serum albumin molecules were more severely decreased (by two orders of magnitude) than those of the nonwoven/polyvinylidene fluoride-polyacrylic acid composite membrane. However, the rejection efficiency of the graphene oxide composite was significantly improved in cationic Rhodamine B (from 9% to 80.3%) and anionic methyl orange (from 28.3% to 86.6%) feed solutions. The folic acid and bovine serum albumin were nearly completely rejected from solutions using either nonwoven/polyvinylidene fluoride-polyacrylic acid or nonwoven/polyvinylidene fluoride-polyacrylic acid/graphene oxide composite membrane, but the latter possessed anti-fouling property against the protein molecules. The separation mechanism in nonwoven/polyvinylidene fluoride-polyacrylic acid membrane includes the Donnan exclusion effect (for smaller-than-pore-size solutes) and sieving mechanism (for larger solutes). The sieving mechanism governs the filtration behavior in the nonwoven/polyvinylidene fluoride-polyacrylic acid/graphene oxide composite membrane.


2017 ◽  
Vol 53 (48) ◽  
pp. 6432-6435 ◽  
Author(s):  
Qian Sun ◽  
Weisi Wang ◽  
Zhaoyang Chen ◽  
Yuhua Yao ◽  
Weibing Zhang ◽  
...  

A reaction-based florescence probe CBF for serum albumin (SA) was proposed by connecting a dioxaborine unit with environment-sensitive coumarin fluorophore. CBF exhibits high selectivity and sensitivity toward SA over other biologically relevant species and has potential of detecting SA in biosamples.


2015 ◽  
Vol 356 ◽  
pp. 844-851 ◽  
Author(s):  
Bing Cai ◽  
Kebang Hu ◽  
Chunming Li ◽  
Jing Jin ◽  
Yuexin Hu

2016 ◽  
Vol 71 (4) ◽  
pp. 277-282 ◽  
Author(s):  
Chien-Hsing Hsu ◽  
Ya-Wei Hsu ◽  
Yu-Ching Weng

AbstractWe have presented a potentiometric urea sensor using an urease/bovine serum albumin (BSA)-poly(3,4-ethylenedioxythiophene)(PEDOT)/Pt electrode. A urea detection sensitivity of 15.2 mV/decade (order of magnitude) has been achieved. BSA trapped in the PEDOT matrix was employed to bond urease molecules on the surface of a BSA-PEDOT/Pt electrode via amide bonds formed between the carboxyl functional groups on the enzyme and the amines on the BSA. The effects of PEDOT thickness, pH value of the urea solution, urease concentration, and temperature on the urea detection sensitivity were also studied. The lifetime of the sensor was studied for a period of 10 weeks, and the average sensing degradation rate was about 9 % per week. This sensor displayed a high selectivity to urea over glucose, KCl, and NaCl.


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