Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

2012 ◽  
Vol 5 (12) ◽  
pp. 9857-9865 ◽  
Author(s):  
Zhengxu Gao ◽  
Hui Zhao ◽  
Zhimin Li ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Carbon Dioxide ◽  
Genetically Engineered ◽  
Photosynthetic Production

scholarly journals Correction: Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria

2016 ◽  
Vol 9 (3) ◽  
pp. 1113-1113 ◽  
Author(s):  
Zhengxu Gao ◽  
Hui Zhao ◽  
Zhimin Li ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Carbon Dioxide ◽  
Genetically Engineered ◽  
Photosynthetic Production ◽  
Energy Environ

Correction for ‘Photosynthetic production of ethanol from carbon dioxide in genetically engineered cyanobacteria’ by Zhengxu Gao et al., Energy Environ. Sci., 2012, 5, 9857–9865.

Photosynthetic Production of Ethanol Using Genetically Engineered Cyanobacteria

2020 ◽  
pp. 99-113
Author(s):  
F. P. De Andrade ◽  
M. L. F. De Sá Filho ◽  
R. R. L. Araújo ◽  
T. R. M. Ribeiro ◽  
A. E. Silva ◽  
...  
Keyword(s):  
Genetically Engineered ◽  
Photosynthetic Production

scholarly journals Enhancing Bioethanol Production by Deleting Phosphoenolpyruvate Synthase and ADP-Glucose Pyrophosphorylase, and Shunting Tricarboxylic Acid Cycle In Synechocystis Sp. PCC 6803

2021 ◽  
Author(s):  
E-Bin Gao ◽  
Penglin Ye ◽  
Haiyan Qiu ◽  
Junhua Wu ◽  
Huayou Chen
Keyword(s):  
Carbon Dioxide ◽  
Ethanol Production ◽  
Atmospheric Carbon Dioxide ◽  
Metabolic Flux ◽  
Tricarboxylic Acid Cycle ◽  
Tricarboxylic Acid ◽  
Acid Cycle ◽  
Photosynthetic Production ◽  
Engineered Strain ◽  
Pcc 6803

Abstract Background: The outstanding ability of directly assimilating carbon dioxide and sunlight to produce biofuels and chemicals impels photosynthetic cyanobacteria to become attractive organisms for the solution to the global warming crises and the world energy growth. The cyanobacteria-based method for ethanol production has been increasingly regarded as alternatives to food biomass-based fermentation and traditional petroleum-based production. Therefore, we engineered the model cyanobacterium Synechocystis sp. PCC 6803 to synthesize ethanol and optimized the biosynthetic pathways for improving ethanol production under photoautotrophic conditions.Results: In this study, we successfully achieved the photosynthetic production of ethanol from atmospheric carbon dioxide by an engineered mutant Synechocystis sp. PCC 6803 with over-expressing the heterologous genes encoding Zymomonas mobilis pyruvate decarboxylase (PDC) and Escherichia coli NADPH-dependent alcohol dehydrogenase (YqhD). The engineered strain was further optimized by an alternative engineering approach to improve cell growth, and increase the intracellular supply of the precursor pyruvate for ethanol production under photoautotrophic conditions. This approach includes blocking phosphoenolpyruvate synthetic pathway from pyruvate, removing glycogen storage, and shunting carbon metabolic flux of tricarboxylic acid cycle. Through redirecting and optimizing the metabolic carbon flux of Synechocystis, a high ethanol-producing efficiency was achieved (248 mg L-1 day-1) under photoautotrophic conditions with atmospheric CO2 as the sole carbon source. Conclusions: The engineered strain SYN009 (∆slr0301/pdc-yqhD, ∆slr1176/maeB) would become a valuable biosystem for photosynthetic production of ethanol and for expanding our knowledge of exploiting cyanobacteria to produce value chemicals directly from atmospheric CO2.

scholarly journals Effects of temperature frequency trends on projected japonica rice (Oryza sativa L.) yield and dry matter distribution with elevated carbon dioxide

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e11027
Author(s):  
Zeyu Zhou ◽  
Jiming Jin ◽  
Libing Song ◽  
Ling Yan
Keyword(s):  
Carbon Dioxide ◽  
Oryza Sativa ◽  
Dry Matter ◽  
Japonica Rice ◽  
Extreme Temperatures ◽  
Matter Distribution ◽  
Dry Matter Distribution ◽  
Rice Growth ◽  
Photosynthetic Production ◽  
Effects Of Temperature

In this study, we investigated the effects of temperature frequency trends on the projected yield and dry matter distribution of japonica rice (Oryza sativa L.) with elevated carbon dioxide (CO2) under future climate change scenarios in northwestern China. The Crop Environment Resource Synthesis (CERES)-Rice model was forced with the outputs from three general circulation models (GCMs) to project the rice growth and yield. Future temperature trends had the most significant impact on rice growth, and the frequency of higher than optimal temperatures (∼24–28 oC) for rice growth showed a marked increase in the future, which greatly restricted photosynthesis. The frequency of extreme temperatures (>35 oC) also increased, exerting a strong impact on rice fertilization and producing a significantly reduced yield. Although the increased temperature suppressed photosynthetic production, the elevated CO2 stimulated this production; therefore, the net result was determined by the dominant process. The aboveground biomass at harvest trended downward when temperature became the major factor in photosynthetic production and trended upward when CO2-fertilization dominated the process. The trends for the leaf and stem dry matter at harvest were affected not only by changes in photosynthesis but also by the dry matter distribution to the panicles. The trends for the rice panicle dry matter at harvest were closely related to the effects of temperature and CO2 on photosynthetic production, and extreme temperatures also remarkably affected these trends by reducing the number of fertilized spikelets. The trends of rice yield were very similar to those of panicle dry matter because the panicle dry matter is mostly composed of grain weight (yield). This study provides a better understanding of the japonica rice processes, particularly under extreme climate scenarios, which will likely become more frequent in the future.

A tubular bioreactor for photosynthetic production of biomass from carbon dioxide: Design and performance

2008 ◽  
Vol 33 (1) ◽  
pp. 35-58 ◽  
Author(s):  
S. John Pirt ◽  
Yuan Kun Lee ◽  
Marek R. Walach ◽  
Margaret Watts Pirt ◽  
Hushang H. M. Balyuzi ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Photosynthetic Production ◽  
Tubular Bioreactor ◽  
And Performance

Photosynthetic production of Dunaliella biomass from natural salt water and carbon dioxide

2008 ◽  
Vol 34 (4) ◽  
pp. 291-295 ◽  
Author(s):  
Teresa I. Cortiñas ◽  
Humberto J. Silva ◽  
Rodolfo J. Ertola
Keyword(s):  
Carbon Dioxide ◽  
Salt Water ◽  
Photosynthetic Production

scholarly journals Enhancing photosynthetic production of ethylene in genetically engineered Synechocystis sp. PCC 6803

2015 ◽  
Vol 17 (1) ◽  
pp. 421-434 ◽  
Author(s):  
Tao Zhu ◽  
Xiaoman Xie ◽  
Zhimin Li ◽  
Xiaoming Tan ◽  
Xuefeng Lu
Keyword(s):  
Ethylene Production ◽  
Genetically Engineered ◽  
Genetic Modifications ◽  
Photosynthetic Production ◽  
Synechocystis Sp ◽  
Pcc 6803

The enhanced ethylene production (9.7 mL L−1h−1) was achieved by genetic modifications and improved cultivation ofSynechocystissp. PCC 6803.

Ecologically significant effects of Pseudomonas putida PPO301(pRO103), genetically engineered to degrade 2,4-dichlorophenoxyacetate, on microbial populations and processes in soil

1991 ◽  
Vol 37 (9) ◽  
pp. 682-691 ◽  
Author(s):  
Jack D. Doyle ◽  
Kevin A. Short ◽  
Guenther Stotzky ◽  
Rick J. King ◽  
Ramon J. Seidler ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Pseudomonas Putida ◽  
Dehydrogenase Activity ◽  
Parental Strain ◽  
Genetically Engineered ◽  
Microbial Populations ◽  
Rate Of Evolution ◽  
Genetically Engineered Microorganisms ◽  
Fungal Propagules ◽  
Genetically Engineered Microorganism

Pseudomonas putida PPO301(pRO103), genetically engineered to degrade 2,4-dichlorophenoxyacetate, affected microbial populations and processes in a nonsterile xeric soil. In soil amended with 2,4-dichlorophenoxyacetate (500 μg/g soil) and inoculated with PPO301(pRO103), the rate of evolution of carbon dioxide was retarded for approximately 35 days; there was a transient increase in dehydrogenase activity; and the number of fungal propagules decreased below detection after 18 days. In unamended soil inoculated with PPO301(pRO103), the rate of evolution of carbon dioxide and the dehydrogenase activity were unaffected, and the numbers of fungal propagules were reduced by about two orders of magnitude. The numbers of total, spore-forming, and chitin-utilizing bacteria were reduced transiently in soil either amended or unamended with 2,4-dichlorophenoxyacetate and inoculated with PPO301(pRO103). The activities of arylsulfatases and phosphatases in soil were not affected by the presence of PPO301(pRO103), either in the presence or absence of 2,4-dichlorophenoxyacetate. In soil amended with 2,4-dichlorophenoxyacetate and inoculated with the parental strain (PPO301) or not inoculated, the evolution of carbon dioxide, the numbers of fungal propagules and of total, spore-forming, and chitin-utilizing bacteria, and the dehydrogenase activity were not affected as in soil inoculated with PPO301(pRO103). These results demonstrated that a genetically engineered microorganism, in the presence of the substrate on which its novel genes can function, is capable of inducing measurable ecological effects in soil. Key words: genetically engineered microorganisms, soil, ecology, 2,4-dichlorophenoxyacetate, Pseudomonas putida.

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