Live cell imaging analysis of the epigenetic regulation of the human endothelial cell migration at single-cell resolution

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3063 ◽  
Author(s):  
Chunhong Zheng ◽  
Zhilong Yu ◽  
Ying Zhou ◽  
Louis Tao ◽  
Yuhong Pang ◽  
...  
Keyword(s):  
Cell Migration ◽  
Endothelial Cell ◽  
Single Cell ◽  
Epigenetic Regulation ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Endothelial Cell Migration ◽  
Human Endothelial Cell ◽  
Imaging Analysis

scholarly journals Monitoring Cannabinoid CB2 -Receptor Mediated cAMP Dynamics by FRET-Based Live Cell Imaging

2020 ◽  
Vol 21 (21) ◽  
pp. 7880
Author(s):  
Leonore Mensching ◽  
Sebastian Rading ◽  
Viacheslav Nikolaev ◽  
Meliha Karsak
Keyword(s):  
Single Cell ◽  
G Protein ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Live Cell ◽  
Single Cell Level ◽  
Cb2 Receptor ◽  
Cell Level ◽  
Cb2 Receptors ◽  
G Protein Coupled

G-protein coupled cannabinoid CB2 receptor signaling and function is primarily mediated by its inhibitory effect on adenylate cyclase. The visualization and monitoring of agonist dependent dynamic 3′,5′-cyclic adenosine monophosphate (cAMP) signaling at the single cell level is still missing for CB2 receptors. This paper presents an application of a live cell imaging while using a Förster resonance energy transfer (FRET)-based biosensor, Epac1-camps, for quantification of cAMP. We established HEK293 cells stably co-expressing human CB2 and Epac1-camps and quantified cAMP responses upon Forskolin pre-stimulation, followed by treatment with the CB2 ligands JWH-133, HU308, β-caryophyllene, or 2-arachidonoylglycerol. We could identify cells showing either an agonist dependent CB2-response as expected, cells displaying no response, and cells with constitutive receptor activity. In Epac1-CB2-HEK293 responder cells, the terpenoid β-caryophyllene significantly modified the cAMP response through CB2. For all of the tested ligands, a relatively high proportion of cells with constitutively active CB2 receptors was identified. Our method enabled the visualization of intracellular dynamic cAMP responses to the stimuli at single cell level, providing insights into the nature of heterologous CB2 expression systems that contributes to the understanding of Gαi-mediated G-Protein coupled receptor (GPCR) signaling in living cells and opens up possibilities for future investigations of endogenous CB2 responses.

scholarly journals Transport of Ebolavirus Nucleocapsids Is Dependent on Actin Polymerization: Live-Cell Imaging Analysis of Ebolavirus-Infected Cells

2015 ◽  
Vol 212 (suppl 2) ◽  
pp. S160-S166 ◽  
Author(s):  
Gordian Schudt ◽  
Olga Dolnik ◽  
Larissa Kolesnikova ◽  
Nadine Biedenkopf ◽  
Astrid Herwig ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Actin Polymerization ◽  
Cell Imaging ◽  
Live Cell ◽  
Imaging Analysis ◽  
Infected Cells
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