Long-Term Live Cell Imaging of Cell Migration: Effects of Pathogenic Fungi on Human Epithelial Cell Migration

Author(s):  
Torsten Wöllert ◽  
George M. Langford
2018 ◽  
Vol 99 ◽  
pp. 683
Author(s):  
Maria P. Walzik ◽  
Verena Vollmar ◽  
Theresa Lachnit ◽  
Helmut Dietz ◽  
Susanne Haug ◽  
...  

Lab on a Chip ◽  
2012 ◽  
Vol 12 (17) ◽  
pp. 3063 ◽  
Author(s):  
Chunhong Zheng ◽  
Zhilong Yu ◽  
Ying Zhou ◽  
Louis Tao ◽  
Yuhong Pang ◽  
...  

Polymer ◽  
2017 ◽  
Vol 118 ◽  
pp. 75-84 ◽  
Author(s):  
Xing Wang ◽  
Yanyu Yang ◽  
Fei Yang ◽  
Hong Shen ◽  
Decheng Wu

IEEE Access ◽  
2018 ◽  
Vol 6 ◽  
pp. 11063-11075 ◽  
Author(s):  
Dhanesh Kattipparambil Rajan ◽  
Joose Kreutzer ◽  
Hannu Valimaki ◽  
Mari Pekkanen-Mattila ◽  
Antti Ahola ◽  
...  

2020 ◽  
Vol 94 (10) ◽  
pp. 3553-3561
Author(s):  
Hauke Reimann ◽  
Helga Stopper ◽  
Henning Hintzsche

Abstract Micronuclei are small nuclear cellular structures containing whole chromosomes or chromosomal fragments. While there is a lot of information available about the origin and formation of micronuclei, less is known about the fate of micronuclei and micronucleated cells. Possible fates include extrusion, degradation, reincorporation and persistence. Live cell imaging was performed to quantitatively analyse the fates of micronuclei and micronucleated cells occurring in vitro. Imaging was conducted for up to 96 h in HeLa-H2B-GFP cells treated with 0.5, 1 and 2 µg/ml etoposide. While a minority of micronuclei was reincorporated into the main nucleus during mitosis, the majority of micronuclei persisted without any alterations. Degradation and extrusion were observed rarely or never. The presence of micronuclei affected the proliferation of the daughter cells and also had an influence on cell death rates. Mitotic errors were found to be clearly increased in micronucleus-containing cells. The results show that micronuclei and micronucleated cells can, although delayed in cell cycle, sustain for multiple divisions.


2005 ◽  
Vol 11 (S02) ◽  
Author(s):  
S Landry ◽  
J P H Th'ng ◽  
B Arjune ◽  
P L McGhee ◽  
W J Keeler

2015 ◽  
Vol 12 (11) ◽  
pp. vii-viii ◽  
Author(s):  
Philip Lee ◽  
Cindy S Y Chen ◽  
Terry Gaige ◽  
Paul J Hung

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