A novel ionic liquid-based monolithic column and its application in the efficient separation of proteins and small molecules by high-performance liquid chromatography

2015 ◽  
Vol 7 (1) ◽  
pp. 218-225 ◽  
Author(s):  
Junxiao Qin ◽  
Ligai Bai ◽  
Jiafei Wang ◽  
Yamin Ma ◽  
Haiyan Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Small Molecules ◽  
High Performance ◽  
Monolithic Column ◽  
High Specific Surface Area ◽  
Excellent Performance ◽  
Efficient Separation ◽  
Porous Monolith ◽  
Separation Of Proteins

Ionic liquid was used to prepare a monolith. The resulting porous monolith had a high specific surface area and was used as the stationary phase for HPLC, showing excellent performance to separate small molecules and protein.

Preparation of a porous polymer monolithic column with an ionic liquid as a porogen and its applications for the separation of small molecules in high performance liquid chromatography

2015 ◽  
Vol 7 (18) ◽  
pp. 7879-7888 ◽  
Author(s):  
Jiafei Wang ◽  
Xiaoya Jiang ◽  
Hang Zhang ◽  
Sha Liu ◽  
Ligai Bai ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Small Molecules ◽  
Small Molecule ◽  
High Performance ◽  
Monolithic Column ◽  
Porous Polymer ◽  
Column Efficiency ◽  
Polymer Monolithic Column

A monolith based on an ionic liquid as a porogen was prepared to enhance the column efficiency of small molecule separation in HPLC.

Simultaneous analysis of nanoparticles and small molecules by high-performance liquid chromatography using a silica monolithic column

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4453-4457 ◽  
Author(s):  
Naoki Itoh ◽  
Akira Sano ◽  
Tomofumi Santa ◽  
Masaru Kato
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Small Molecules ◽  
High Performance ◽  
Monolithic Column ◽  
Simultaneous Analysis ◽  
Bimodal Structure ◽  
Simultaneous Separation

The bimodal structure of the monolithic column is promising for the simultaneous separation, because nanoparticles and small molecules were separated by micrometer- and nanometer-sized pores, respectively.

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