Preparation of a porous polymer monolithic column with an ionic liquid as a porogen and its applications for the separation of small molecules in high performance liquid chromatography

2015 ◽  
Vol 7 (18) ◽  
pp. 7879-7888 ◽  
Author(s):  
Jiafei Wang ◽  
Xiaoya Jiang ◽  
Hang Zhang ◽  
Sha Liu ◽  
Ligai Bai ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Small Molecules ◽  
Small Molecule ◽  
High Performance ◽  
Monolithic Column ◽  
Porous Polymer ◽  
Column Efficiency ◽  
Polymer Monolithic Column

A monolith based on an ionic liquid as a porogen was prepared to enhance the column efficiency of small molecule separation in HPLC.

Simultaneous analysis of nanoparticles and small molecules by high-performance liquid chromatography using a silica monolithic column

The Analyst ◽  
2014 ◽  
Vol 139 (18) ◽  
pp. 4453-4457 ◽  
Author(s):  
Naoki Itoh ◽  
Akira Sano ◽  
Tomofumi Santa ◽  
Masaru Kato
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Small Molecules ◽  
High Performance ◽  
Monolithic Column ◽  
Simultaneous Analysis ◽  
Bimodal Structure ◽  
Simultaneous Separation

The bimodal structure of the monolithic column is promising for the simultaneous separation, because nanoparticles and small molecules were separated by micrometer- and nanometer-sized pores, respectively.

A novel ionic liquid-based monolithic column and its application in the efficient separation of proteins and small molecules by high-performance liquid chromatography

2015 ◽  
Vol 7 (1) ◽  
pp. 218-225 ◽  
Author(s):  
Junxiao Qin ◽  
Ligai Bai ◽  
Jiafei Wang ◽  
Yamin Ma ◽  
Haiyan Liu ◽  
...  
Keyword(s):  
Ionic Liquid ◽  
High Performance Liquid Chromatography ◽  
Small Molecules ◽  
High Performance ◽  
Monolithic Column ◽  
High Specific Surface Area ◽  
Excellent Performance ◽  
Efficient Separation ◽  
Porous Monolith ◽  
Separation Of Proteins

Ionic liquid was used to prepare a monolith. The resulting porous monolith had a high specific surface area and was used as the stationary phase for HPLC, showing excellent performance to separate small molecules and protein.

scholarly journals Preparation of Poly-(GMA-EDA-β-CD-co-TMPTMA) Monolith as High Performance Liquid Chromatography Chiral Stationary Phase Column

2019 ◽  
Author(s):  
Stevin Carolius Angga ◽  
Dias Septiana ◽  
Suci Amalia ◽  
Warsito Warsito ◽  
Elvina Dhiaul Iftitah ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Stationary Phase ◽  
High Performance ◽  
Chiral Stationary Phase ◽  
Monolithic Column ◽  
Organic Polymer ◽  
Polymer Monolithic Column ◽  
Chiral Atom ◽  
Phase Column

An enantiomer molecule consisted of the chiral atom has different structure conformations, which exhibit different activities as well. Yet, its separation considerably difficult since ordinary separation could not separate both molecules. One of the popular enantioseparations which are often used was using organic polymer monolithic column modified by ethylenediamine-β-cyclodextrin (EDA-β-CD) as the enantioseparations site. The aim of this research was to produce chiral stationary phase column for enantioseparations of (±)-citronellal. It was conducted by preparing monolithic column using monomer glycidyl methacrylate (GMA), trimethylolpropane trimethacrylate (TMPTMA) as crosslinker, 1-propanol, 1,4-butanediol, and water as pore-forming agents (porogens) in the presence of α,α'-azoisobutyronitrile (AIBN) as radical initiator inside polyetheretherketone (PEEK) tubing. It was then modified with EDA-β-CD synthesized from β-CD. Finally, it was installed as a high-performance liquid chromatography column. The result shows the produced chiral stationary phase column could separate (±)-citronellal at a retention time of 44.76 and 45.71 min.

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