Side chain thiol-functionalized poly(ethylene glycol) by post-polymerization modification of hydroxyl groups: synthesis, crosslinking and inkjet printing

2014 ◽  
Vol 5 (18) ◽  
pp. 5350-5359 ◽  
Author(s):  
Alexander Southan ◽  
Eva Hoch ◽  
Veronika Schönhaar ◽  
Kirsten Borchers ◽  
Christian Schuh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Inkjet Printing ◽  
Functionalized Polymers ◽  
Side Chain ◽  
Hydroxyl Groups ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Thiol functionalized PEG-based polymers were synthesized by post polymerization reactions of hydroxyl functionalized polymers. Applications of the polymers in cell culture and inkjet printing were demonstrated.

The Rapid Fabrication of Hydrogel Microfluidic Chip for Cell Capture Culture and Metabolites Detection

2013 ◽  
Vol 562-565 ◽  
pp. 632-636
Author(s):  
Ke Jing Fang ◽  
Chang Jun Hou ◽  
Cheng Hong Huang ◽  
Xiao Gang Luo ◽  
Su Yi Zhang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Microfluidic Chip ◽  
Cell Biology ◽  
Large Scale ◽  
Polymer Network ◽  
Hydroxyl Groups ◽  
Cell Capture ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

The microfluidic chip with well-defined structure is an important platform for cell research. The existing techniques for chip fabrication especially in cell biology and tissue engineering have many defects, for example, poor processing precision, high processing cost, as well as sophisticated manufacturing procedure. Thus, fabrication of simple and practicable microfluidic chip with highly efficient cell control ability and low-cost is turned to be the main target for bioengineering application. Poly(ethylene glycol) (PEG) is a hydrophilic polymer. Substituting terminal hydroxyl groups with acrylates, forming poly(ethylene glycol) diacrylate (PEGDA), allows the polymer to be cross-linked to form a three-dimensional polymer network. Meanwhile the use of photopolymerization can realize precise and temporal control of polymerization for formation of complex shapes. Herein, we utilize PEGDA hydrogel’s highly tunable characteristic, using photopolymerization method to obtain desirable micro-structure. Each chip has four of uniform micro-structures, which can carry multiple parallel experiments at the same time. We also add 2-Hydroxyethyl Methacrylate (HEMA) to the PEGDA prepolymer in order to increase the cell adhesion capacity of the microchip surface for cell culture. The experimental results showed that this method can achieve double-layer cell culture with short time treatment. Cells can be well captured and cultured in the hydrogel microfluidic chip with excellent activity. The hydrogel microfluidic chip has the potential of practicable application once large-scale preparation is accomplished.

Preparation and characterization of methoxy-poly(ethylene glycol) side chain grafted onto chitosan as a wound dressing film

2015 ◽  
Vol 132 (31) ◽  
pp. n/a-n/a ◽  
Author(s):  
Cheng-Han Yang ◽  
Szu-Hsien Chen ◽  
Yun-Wen Pan ◽  
Ching-Nan Chuang ◽  
Wen-Chi Chao ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Wound Dressing ◽  
Side Chain ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Tailorable Cell Culture Platforms from Enzymatically Cross-Linked Multifunctional Poly(ethylene glycol)-Based Hydrogels

2013 ◽  
Vol 14 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Donna J. Menzies ◽  
Andrew Cameron ◽  
Trent Munro ◽  
Ernst Wolvetang ◽  
Lisbeth Grøndahl ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

Interfacial polycondensation-derived side-chain poly(ethylene glycol)-containing water-soluble polysulfide weak-link polymers as stabilizer for gold nanoparticles

2017 ◽  
Vol 115 ◽  
pp. 10-17 ◽  
Author(s):  
Ujjal Haldar ◽  
Kapil Dev Sayala ◽  
Kannan Sivaprakasam ◽  
Latha Ramakrishnan ◽  
Priyadarsi De
Keyword(s):  
Gold Nanoparticles ◽  
Ethylene Glycol ◽  
Weak Link ◽  
Water Soluble ◽  
Side Chain ◽  
Interfacial Polycondensation ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Biofunctionalization of Metal Surface by Immobilization of Poly(Ethylene Glycol) Terminated Amine

2007 ◽  
Vol 26-28 ◽  
pp. 765-768
Author(s):  
Yuta Tanaka ◽  
Yuh Matsuo ◽  
Haruka Saito ◽  
Yusuke Tsutsumi ◽  
Hisashi Doi ◽  
...  
Keyword(s):  
Ethylene Glycol ◽  
Metal Surface ◽  
Photoelectron Spectroscopy ◽  
Blood Compatibility ◽  
Pure Titanium ◽  
Hydroxyl Groups ◽  
Commercially Pure Titanium ◽  
Cobalt Chromium ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

In many biomedical devices such as catheters and diagnostic sensors, blood compatibility is required. The best way to control this property is to prevent or drastically reduce the adsorption of proteins. Poly(ethylene glycol) terminated amine at both terminals, NH2-PEG-NH2, is immobilized on a commercially pure titanium, a 316L austenitic stainless steel, and a cobalt-chromium-molybdenum alloy with immersion or electrodeposition. Chemical bonding states at the interface and orientation of PEG molecules were characterized using X-ray photoelectron spectroscopy, glow discharge optical emission spectroscopy, and Fourie-transformed infrared spectrometer with a reflection absorption spectrometer. As a result, NH2-PEG-NH2 was immobilized onto metal surface as a U-shape mainly with stable NHO bonding in electrodeposition. In the case of electrodepostion, the concentration of active surface hydroxyl groups on surface oxide film played an important role in the immobilization.

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