Tailorable Cell Culture Platforms from Enzymatically Cross-Linked Multifunctional Poly(ethylene glycol)-Based Hydrogels

2013 ◽  
Vol 14 (2) ◽  
pp. 413-423 ◽  
Author(s):  
Donna J. Menzies ◽  
Andrew Cameron ◽  
Trent Munro ◽  
Ernst Wolvetang ◽  
Lisbeth Grøndahl ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals A Non-Cytotoxic Resin for Micro-Stereolithography for Cell Cultures of HUVECs

Micromachines ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 246 ◽  
Author(s):  
Max Männel ◽  
Carolin Fischer ◽  
Julian Thiele
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Umbilical Vein ◽  
Polymer Materials ◽  
Poly Ethylene Glycol ◽  
Glycol Diacrylate ◽  
3D Printed ◽  
Poly Ethylene ◽  
The Impact

Three-dimensional (3D) printing of microfluidic devices continuously replaces conventional fabrication methods. A versatile tool for achieving microscopic feature sizes and short process times is micro-stereolithography (µSL). However, common resins for µSL lack biocompatibility and are cytotoxic. This work focuses on developing new photo-curable resins as a basis for µSL fabrication of polymer materials and surfaces for cell culture. Different acrylate- and methacrylate-based compositions are screened for material characteristics including wettability, surface roughness, and swelling behavior. For further understanding, the impact of photo-absorber and photo-initiator on the cytotoxicity of 3D-printed substrates is studied. Cell culture experiments with human umbilical vein endothelial cells (HUVECs) in standard polystyrene vessels are compared to 3D-printed parts made from our library of homemade resins. Among these, after optimizing material composition and post-processing, we identify selected mixtures of poly(ethylene glycol) diacrylate (PEGDA) and poly(ethylene glycol) methyl ethyl methacrylate (PEGMEMA) as most suitable to allow for fabricating cell culture platforms that retain both the viability and proliferation of HUVECs. Next, our PEGDA/PEGMEMA resins will be further optimized regarding minimal feature size and cell adhesion to fabricate microscopic (microfluidic) cell culture platforms, e.g., for studying vascularization of HUVECs in vitro.

Enzyme-mediated preparation of hydrogels composed of poly(ethylene glycol) and gelatin as cell culture platforms

RSC Advances ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 3070-3073 ◽  
Author(s):  
Kousuke Moriyama ◽  
Rie Wakabayashi ◽  
Masahiro Goto ◽  
Noriho Kamiya
Keyword(s):  
Cell Culture ◽  
Horseradish Peroxidase ◽  
Ethylene Glycol ◽  
Cell Sheet ◽  
Viable Cell ◽  
Poly Ethylene Glycol ◽  
Redox Responsive ◽  
Poly Ethylene ◽  
Responsive Hydrogels

Horseradish peroxidase-mediated preparation of redox-responsive hydrogels composed of thiolated poly(ethylene glycol) and gelatin allowed the proliferation of cells on the gel and the harvest as a viable cell sheet.

Side chain thiol-functionalized poly(ethylene glycol) by post-polymerization modification of hydroxyl groups: synthesis, crosslinking and inkjet printing

2014 ◽  
Vol 5 (18) ◽  
pp. 5350-5359 ◽  
Author(s):  
Alexander Southan ◽  
Eva Hoch ◽  
Veronika Schönhaar ◽  
Kirsten Borchers ◽  
Christian Schuh ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Inkjet Printing ◽  
Functionalized Polymers ◽  
Side Chain ◽  
Hydroxyl Groups ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

Thiol functionalized PEG-based polymers were synthesized by post polymerization reactions of hydroxyl functionalized polymers. Applications of the polymers in cell culture and inkjet printing were demonstrated.

Hydrophilic poly (ethylene glycol) coating on PDLLA/BCP bone scaffold for drug delivery and cell culture

2008 ◽  
Vol 28 (1) ◽  
pp. 141-149 ◽  
Author(s):  
Li Fang Zhang ◽  
Rui Sun ◽  
Liang Xu ◽  
Jian Du ◽  
Zuo Chun Xiong ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Cell Culture ◽  
Ethylene Glycol ◽  
Bone Scaffold ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

scholarly journals Novel Micropaterned Surface Fabricated from Heterobifunctional Poly(ethylene glycol)/polylactide Block Copolymers for Patterned Cell Culture.

2001 ◽  
Vol 14 (1) ◽  
pp. 101-104 ◽  
Author(s):  
Hidenori Otsuka ◽  
Yukio Nagasaki ◽  
Yasuhiro Horiike ◽  
Teruo Okano ◽  
Kazunori Kataoka
Keyword(s):  
Cell Culture ◽  
Block Copolymers ◽  
Ethylene Glycol ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

The Rapid Fabrication of Hydrogel Microfluidic Chip for Cell Capture Culture and Metabolites Detection

2013 ◽  
Vol 562-565 ◽  
pp. 632-636
Author(s):  
Ke Jing Fang ◽  
Chang Jun Hou ◽  
Cheng Hong Huang ◽  
Xiao Gang Luo ◽  
Su Yi Zhang ◽  
...  
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
Microfluidic Chip ◽  
Cell Biology ◽  
Large Scale ◽  
Polymer Network ◽  
Hydroxyl Groups ◽  
Cell Capture ◽  
Poly Ethylene Glycol ◽  
Poly Ethylene

The microfluidic chip with well-defined structure is an important platform for cell research. The existing techniques for chip fabrication especially in cell biology and tissue engineering have many defects, for example, poor processing precision, high processing cost, as well as sophisticated manufacturing procedure. Thus, fabrication of simple and practicable microfluidic chip with highly efficient cell control ability and low-cost is turned to be the main target for bioengineering application. Poly(ethylene glycol) (PEG) is a hydrophilic polymer. Substituting terminal hydroxyl groups with acrylates, forming poly(ethylene glycol) diacrylate (PEGDA), allows the polymer to be cross-linked to form a three-dimensional polymer network. Meanwhile the use of photopolymerization can realize precise and temporal control of polymerization for formation of complex shapes. Herein, we utilize PEGDA hydrogel’s highly tunable characteristic, using photopolymerization method to obtain desirable micro-structure. Each chip has four of uniform micro-structures, which can carry multiple parallel experiments at the same time. We also add 2-Hydroxyethyl Methacrylate (HEMA) to the PEGDA prepolymer in order to increase the cell adhesion capacity of the microchip surface for cell culture. The experimental results showed that this method can achieve double-layer cell culture with short time treatment. Cells can be well captured and cultured in the hydrogel microfluidic chip with excellent activity. The hydrogel microfluidic chip has the potential of practicable application once large-scale preparation is accomplished.

Enzymatically cross-linked hydrogels based on a linear poly(ethylene glycol) analogue for controlled protein release and 3D cell culture

2018 ◽  
Vol 6 (38) ◽  
pp. 6067-6079 ◽  
Author(s):  
Danni Wang ◽  
Xiaowei Yang ◽  
Qiong Liu ◽  
Lin Yu ◽  
Jiandong Ding
Keyword(s):  
Cell Culture ◽  
Ethylene Glycol ◽  
3D Cell Culture ◽  
Protein Release ◽  
Poly Ethylene Glycol ◽  
Delivery Platform ◽  
Linear Poly ◽  
Poly Ethylene

An injectable PEG-based hydrogel is designed using an enzyme as a catalyst, and its potential as a delivery platform for proteins and cells is demonstrated.

Degradation and cell culture studies on block copolymers prepared by ring opening polymerization of ?-caprolactone in the presence of poly(ethylene glycol)

2004 ◽  
Vol 69A (3) ◽  
pp. 417-427 ◽  
Author(s):  
Ming-Hsi Huang ◽  
Suming Li ◽  
Dietmar Werner Hutmacher ◽  
Jan-Thorsten Schantz ◽  
Charles Alfred Vacanti ◽  
...  
Keyword(s):  
Cell Culture ◽  
Block Copolymers ◽  
Ethylene Glycol ◽  
Ring Opening ◽  
Ring Opening Polymerization ◽  
Poly Ethylene Glycol ◽  
Culture Studies ◽  
Poly Ethylene
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