scholarly journals Controlling colloidal stability of silica nanoparticles during bioconjugation reactions with proteins and improving their longer-term stability, handling and storage

2015 ◽  
Vol 3 (10) ◽  
pp. 2043-2055 ◽  
Author(s):  
C. J. Moore ◽  
H. Montón ◽  
R. O'Kennedy ◽  
D. E. Williams ◽  
C. Nogués ◽  
...  
Keyword(s):  
Silica Nanoparticles ◽  
Colloidal Stability ◽  
Improved Method ◽  
Term Stability ◽  
Long Term Stability ◽  
And Storage

Robust protocols for antibody-nanoparticle (Ab-NP) conjugation, and improved method for long-term stability and storage of Ab-NPs using cryoprotectants.

Preparation of Glucose Sensor Using Polydimethylsiloxane / Polypyrrole Complex

2003 ◽  
Vol 17 (08n09) ◽  
pp. 1217-1222 ◽  
Author(s):  
Mikito Yasuzawa ◽  
Shigeru Inoue ◽  
Shinji Imai
Keyword(s):  
Glucose Sensor ◽  
Platelet Rich Plasma ◽  
Glucose Sensors ◽  
Wire Electrode ◽  
Term Stability ◽  
Long Term Stability ◽  
Response Current ◽  
And Storage ◽  
Storage Temperatures

New glucose oxidase (GOD) immobilized glucose sensors were prepared by the electropolymerization of 1-(6-D-gluconamidohexyl) pyrrole (GHP) on the platinum wire electrode precoated with the mixture solution of pyrrole derivative GHP, polydimethylsiloxane (PDS) and Nafion. The addition of Nafion into the precoating mixture solution was essential to obtain suitable sensor sensitivity. However, the sensitivity was about the half of that of the electrode without PDS precoating. Although, the introduction of Nafion was effective to improve the long-term stability of the enzyme-immobilized electrode, the electrode prepared using Nafion, PDS and GHP performed excellent long-term stability even at the measurement and storage temperatures of 40°C. Relatively constant response current was obtained over 30 days under the condition of 40°C and over 9 months measured at 25°C. Moreover, the GOD-immobilized GHP polymer film prepared on the electrode precoated with GHP, PDS and Nafion solution, was found to have excellent hemocompatibility from the result of platelet rich plasma contacting test.

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