Colorimetric detection of influenza A virus using antibody-functionalized gold nanoparticles

The Analyst ◽  
2015 ◽  
Vol 140 (12) ◽  
pp. 3989-3995 ◽  
Author(s):  
Yuanjian Liu ◽  
Linqun Zhang ◽  
Wei Wei ◽  
Hongyu Zhao ◽  
Zhenxian Zhou ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Influenza A Virus ◽  
Clinical Diagnosis ◽  
Specific Antibody ◽  
Influenza A ◽  
Colorimetric Detection ◽  
Viral Pathogen ◽  
Virus Surface ◽  
Functionalized Gold Nanoparticles

A colorimetric immunosensor for IAV based on AuNPs modified with mAb is developed. This assay depends on an ordered AuNPs structure covering the virus surface and can be applied to any viral pathogen by incorporating the appropriate pathogen-specific antibody, giving the proposed method a broad prospect in clinical diagnosis applications.

scholarly journals Multivalent Sialyllactose-Levan-Conjugated Gold Nanoparticles for Efficient Interaction with and Colorimetric Detection of Influenza A Virus

Chemosensors ◽  
2021 ◽  
Vol 9 (7) ◽  
pp. 186
Author(s):  
Sun-Jung Kim ◽  
Pan Kee Bae ◽  
Yong-Beom Shin
Keyword(s):  
Gold Nanoparticles ◽  
Influenza Virus ◽  
Influenza A Virus ◽  
Influenza A ◽  
Limit Of Detection ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
H1n1 Influenza ◽  
Human Influenza ◽  
Human Influenza Virus

We report a colorimetric assay to detect influenza A virus using sialyllactose-levan-conjugated gold nanoparticles (AuNPs). We successfully conjugated 2, 3- and 2, 6-sialyllactose to levan and synthesized sialyllactose-levan-conjugated AuNPs. Each sialyllactose-conjugated levan specifically interacted with a recognizable lectin. Synthesized sialyllactose-conjugated levan acted as reducing and coating agents during the formation of AuNPs. Human influenza A virus specifically bound to 2, 6-sialyllactose-levan-conjugated AuNPs. Moreover, 2, 6-sialyllactose-conjugated levan AuNPs rapidly changed color from red to blue after incubation with human influenza virus. For detecting avian influenza virus, 2, 3-sialyllactose-levan-conjugated AuNPs were more effective than 2, 6-sialyllactose-levan-conjugated AuNPs. Therefore, the efficient targeting and diagnosis of influenza virus according to origin was possible. The deployment of sialyllactose-levan-conjugated particles for the detection of influenza virus is simple and quick. The limit of detection (L.O.D) of H1N1 influenza virus was 7.4 × 103 pfu using 2, 6-siallylactose-levan-conjugated AuNPs and H5N2 influenza virus was 4.2 × 103 pfu using 2, 3-siallylactose-levan- conjugated AuNPs.

Rapid colorimetric detection of arsenic (III) by glutathione functionalized gold nanoparticles based on RGB extracting system

2021 ◽  
Vol 133 ◽  
pp. 106522
Author(s):  
Biao Zheng ◽  
Jing Li ◽  
Zhongkai Zheng ◽  
Cheng Zhang ◽  
Chunlei Huang ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Functionalized Gold Nanoparticles

L-cysteine functionalized gold nanoparticles for the colorimetric detection of Hg2+induced by ultraviolet light

2009 ◽  
Vol 21 (2) ◽  
pp. 025501 ◽  
Author(s):  
Fang Chai ◽  
Chungang Wang ◽  
Tingting Wang ◽  
Zhanfang Ma ◽  
Zhongmin Su
Keyword(s):  
Gold Nanoparticles ◽  
Ultraviolet Light ◽  
Colorimetric Detection ◽  
Functionalized Gold Nanoparticles

Peptide functionalized gold nanoparticles for colorimetric detection of matrilysin (MMP-7) activity

Nanoscale ◽  
2013 ◽  
Vol 5 (19) ◽  
pp. 8973 ◽  
Author(s):  
Peng Chen ◽  
Robert Selegård ◽  
Daniel Aili ◽  
Bo Liedberg
Keyword(s):  
Gold Nanoparticles ◽  
Colorimetric Detection ◽  
Functionalized Gold Nanoparticles

scholarly journals Role of Memory B Cells in Hemagglutinin-Specific Antibody Production Following Human Influenza A Virus Infection

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 167 ◽  
Author(s):  
Mark Y. Sangster ◽  
Phuong Q. T. Nguyen ◽  
David J. Topham
Keyword(s):  
B Cells ◽  
Virus Infection ◽  
Antibody Response ◽  
Influenza A Virus ◽  
Antibody Production ◽  
Specific Antibody ◽  
Germinal Center ◽  
Influenza A ◽  
Influenza Virus Infection

When influenza A virus infects an immune individual, preexisting memory B cell (MBC) activation and rapid anamnestic antibody production plays a key role in viral clearance. The most effective neutralizing antibodies target the antigenically variable head of the viral hemagglutinin (HA); antibodies against the conserved HA stalk provide broader but less potent protection. In this review, we provide a comprehensive picture of an adult’s HA-specific antibody response to influenza virus infection. The process is followed from preexisting HA-specific MBC activation and rapid production of anti-HA antibodies, through to germinal center seeding and adaptation of the response to novel features of the HA. A major focus of the review is the role of competition between preexisting MBCs in determining the character of the HA-reactive antibody response. HA novelty modifies this competition and can shift the response from the immunodominant head to the stalk. We suggest that antibodies resulting from preexisting MBC activation are important regulators of anti-HA antibody production and play a role in positive selection of germinal center B cells reactive to novel HA epitopes. Our review also considers the role of MBCs in the effects of early-life imprinting on HA head- and stalk-specific antibody responses to influenza infection. An understanding of the processes described in this review will guide development of vaccination strategies that provide broadly effective protection.

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