Visual detection of serum asialohaptoglobin by plasmonic sandwich ELLSA – a new platform for cirrhosis diagnosis

The Analyst ◽  
2016 ◽  
Vol 141 (1) ◽  
pp. 76-84 ◽  
Author(s):  
Partha Pratim Bose ◽  
Gautam Mandal ◽  
Dharmendra Kumar ◽  
Ajay Duseja ◽  
Bishnu Pada Chatterjee
Keyword(s):  
Liver Cirrhosis ◽  
High Throughput ◽  
Visual Detection ◽  
Point Of Care ◽  
Visual Assay

A cheap, fast, point-of-care and high throughput visual assay platform for estimation of asialo-Haptoglobin to monitor liver cirrhosis.

scholarly journals INSIGHT: A population-scale COVID-19 testing strategy combining point-of-care diagnosis with centralized high-throughput sequencing

2021 ◽  
Vol 7 (7) ◽  
pp. eabe5054
Author(s):  
Qianxin Wu ◽  
Chenqu Suo ◽  
Tom Brown ◽  
Tengyao Wang ◽  
Sarah A. Teichmann ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Sequencing ◽  
Limit Of Detection ◽  
Point Of Care ◽  
Population Level ◽  
Reaction Products ◽  
Testing Strategy ◽  
Asymptomatic Patients ◽  
Testing Stage ◽  
Population Scale

We present INSIGHT [isothermal NASBA (nucleic acid sequence–based amplification) sequencing–based high-throughput test], a two-stage coronavirus disease 2019 testing strategy, using a barcoded isothermal NASBA reaction. It combines point-of-care diagnosis with next-generation sequencing, aiming to achieve population-scale testing. Stage 1 allows a quick decentralized readout for early isolation of presymptomatic or asymptomatic patients. It gives results within 1 to 2 hours, using either fluorescence detection or a lateral flow readout, while simultaneously incorporating sample-specific barcodes. The same reaction products from potentially hundreds of thousands of samples can then be pooled and used in a highly multiplexed sequencing–based assay in stage 2. This second stage confirms the near-patient testing results and facilitates centralized data collection. The 95% limit of detection is <50 copies of viral RNA per reaction. INSIGHT is suitable for further development into a rapid home-based, point-of-care assay and is potentially scalable to the population level.

scholarly journals High-throughput roll-to-roll production of polymer biochips for multiplexed DNA detection in point-of-care diagnostics

Lab on a Chip ◽  
2020 ◽  
Vol 20 (22) ◽  
pp. 4106-4117
Author(s):  
Pelin Toren ◽  
Martin Smolka ◽  
Anja Haase ◽  
Ursula Palfinger ◽  
Dieter Nees ◽  
...  
Keyword(s):  
High Throughput ◽  
Nanoimprint Lithography ◽  
Dna Detection ◽  
Point Of Care ◽  
Roll To Roll ◽  
Flexible Polymer ◽  
Nano Structures ◽  
Point Of Care Diagnostics ◽  
Polymer Foils

Roll-to-roll UV nanoimprint lithography has superior advantages for high-throughput manufacturing of micro- or nano-structures on flexible polymer foils with various geometries and configurations.

scholarly journals Paper-Based SERS Platform for One-Step Screening of Tetracycline in Milk

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ana Marques ◽  
Bruno Veigas ◽  
Andreia Araújo ◽  
Beatriz Pagará ◽  
Pedro Viana Baptista ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Point Of Care ◽  
Dairy Industry ◽  
Principal Component ◽  
Surface Enhanced Raman Spectroscopy ◽  
Economic Losses ◽  
Screening Methods ◽  
Sers Substrate ◽  
Molecular Signatures

AbstractThroughout the last decade, the expansion of food testing has been gradually moving towards ordinary high throughput screening methods performed on-site. The demand for point-of-care testing, able to distinguish molecular signatures with high accuracy, sensitivity and specificity has been significantly increasing. This new requirement relies on the on-site detection and monitorization of molecular signatures suitable for the surveillance of food production and processing. The widespread use of antibiotics has contributed to disease control of livestock but has also created problems for the dairy industry and consumers. Its therapeutic and subtherapeutic use has increased the risk of contamination in milk in enough concentrations to cause economic losses to the dairy industry and have a health impact in highly sensitive individuals. This study focuses on the development of a simple Surface-Enhanced Raman Spectroscopy (SERS) method for fast high throughput screening of tetracycline (TET) in milk. For this, we integrate a paper-based low-cost, fully recyclable and highly stable SERS platform, with a minimal sample preparation protocol. A two-microliter sample of milk solutions spiked with TET (from 0.01 to 1000 ppm) is dried on a silver nanoparticle coated cardboard substrate and measured via a Raman spectrophotometer. The SERS substrate showed to be extremely stable with a shelf life of several months. A global spectrum principal component analysis approach was used to test all the detected vibrational modes and their correlation with TET concentration. Peak intensity ratios (455 cm−1/1280 cm−1 and 874 cm−1/1397 cm−1) were found to be correlated with TET concentrations in milk, achieving a sensitivity as low as 0.1 ppm. Results indicate that this SERS method combined with portable Raman spectrometer is a potential tool that can be used on-site for the monitoring of TET residues and other antibiotics.

scholarly journals Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection

2020 ◽  
Vol 169 ◽  
pp. 112642
Author(s):  
Yanju Chen ◽  
Ya Shi ◽  
Yin Chen ◽  
Zhangnv Yang ◽  
Hui Wu ◽  
...  
Keyword(s):  
Visual Detection ◽  
Point Of Care ◽  
Promising Method ◽  
Point Of Care Detection

Towards Point of Care Diagnostics: Visual Detection of Meningitis Pathogens Directly from Cerebrospinal Fluid

2020 ◽  
Vol 5 (46) ◽  
pp. 14572-14577
Author(s):  
Evgeny A. Kovtunov ◽  
Liubov A. Shkodenko ◽  
Ekaterina A. Goncharova ◽  
Daria D. Nedorezova ◽  
Sergey V. Sidorenko ◽  
...  
Keyword(s):  
Cerebrospinal Fluid ◽  
Visual Detection ◽  
Point Of Care ◽  
Point Of Care Diagnostics

Fast and Ultrasensitive Visual Detection of Exosomes in Body Fluids for Point-of-Care Disease Diagnosis

2021 ◽  
Author(s):  
Shan Chen ◽  
Tao Jiang ◽  
Hao Lin ◽  
Junyan Chen ◽  
Shuangli Yang ◽  
...  
Keyword(s):  
Visual Detection ◽  
Point Of Care ◽  
Disease Diagnosis ◽  
Body Fluids

scholarly journals Oximetry-detected pulsus paradoxus predicts for severity in paediatric asthma

2020 ◽  
Vol 105 (6) ◽  
pp. 533-538 ◽  
Author(s):  
Sandhya G Krishnan ◽  
Hung Chew Wong ◽  
Sashikumar Ganapathy ◽  
Gene Yong-Kwang Ong
Keyword(s):  
Visual Detection ◽  
Point Of Care ◽  
Severe Respiratory Distress ◽  
Initial Management ◽  
Paediatric Asthma ◽  
Pulsus Paradoxus ◽  
Relative Risks ◽  
High Dependency ◽  
Before And After ◽  
Outcomes For Children

ObjectiveTo evaluate if qualitative visual detection of pulsus paradoxus (PP) on the pulse oximeter plethysmograph can predict outcomes for children with moderate to severe respiratory distress in a paediatric emergency department (ED).DesignProspective cohort study.SettingPaediatric ED of a tertiary paediatrics hospital in Singapore.PatientsChildren managed for moderate to severe wheezing in the resuscitation bay of the ED.InterventionsPatients were assessed for the presence of PP based on visual detection of oximeter plethysmograph before and after initial inhaled bronchodilator therapy.Main outcome measuresThese include the need for adjunct medications such as aminophylline or magnesium sulfate, the need for supplementary ventilation and the need for admission to the high dependency unit (HDU) or intensive care unit (ICU).ResultsThere were 285 patients included in the study, of whom 78 (27.4%) had PP at ED presentation. There were 40 (14.0%) who had PP after initial management. Children who had PP after initial management had significantly relative risks (RR) of requiring adjunct medications (RR 12.5, 95% CI 4.0 to 38.6), need for supplementary ventilation (RR 5.6, 95% CI 1.2 to 26.5) and admission to the HDU/ICU (RR 5.6, 95% CI 3.0 to 10.4).ConclusionQualitative detection of PP on pulse oximetry can be used as a potential point-of-care tool to help in the assessment of response to initial treatment in paediatric patients with acute moderate to severe asthma exacerbations. Future studies are needed to assess and validate its role in guiding ED management of acute paediatric asthma.

scholarly journals Direct Comparison of SARS-CoV-2 Analytical Limits of Detection across Seven Molecular Assays

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Becky Fung ◽  
Allan Gopez ◽  
Venice Servellita ◽  
Shaun Arevalo ◽  
Coral Ho ◽  
...  
Keyword(s):  
High Throughput ◽  
Extraction Method ◽  
Point Of Care ◽  
Digital Pcr ◽  
Droplet Digital Pcr ◽  
Analytical Sensitivity ◽  
Limits Of Detection ◽  
Molecular Assays ◽  
Performance Metric ◽  
Roche Cobas

ABSTRACT Analytical sensitivity for SARS-CoV-2 detection is a key performance metric for the evaluation of viral detection assays. We determined analytical limits of detection for seven SARS-CoV-2 assays using serial dilutions of pooled patient material quantified with droplet digital PCR. Limits of detection ranged from ≤10 to 74 copies/ml for commercial high-throughput laboratory analyzers (Roche Cobas, Abbott m2000, and Hologic Panther Fusion) and 167 to 511 copies/ml for sample-to-answer (DiaSorin Simplexa, GenMark ePlex) and point-of-care instruments (Abbott ID NOW). The CDC assay yielded limits of detection ranging from 85 to 499 copies/ml, depending on the extraction method and thermocycler used. These results can help to inform the assay choice for testing approaches to manage the current COVID-19 outbreak.

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