A review of traditional and emerging methods to characterize lipid–protein interactions in biological membranes

2015 ◽  
Vol 7 (17) ◽  
pp. 7076-7094 ◽  
Author(s):  
Chih-Yun Hsia ◽  
Mark J. Richards ◽  
Susan Daniel
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Protein Interactions ◽  
Protein Structures ◽  
Biological Membranes ◽  
Biological Functions ◽  
Cell Plasma Membrane ◽  
Lipid Interactions ◽  
Cell Plasma ◽  
Lipid Protein Interactions

Lipid–protein interactions are essential for modulating membrane protein structures and biological functions in the cell plasma membrane. In this review we describe the salient features of classical and emerging methodologies for studying protein–lipid interactions and their limitations.

scholarly journals Lipid-Protein Interactions in Fiber Cell Plasma Membrane Isolated From Human and Porcine Eye Lenses

2017 ◽  
Vol 112 (3) ◽  
pp. 319a
Author(s):  
Marija Raguz ◽  
Laxman Mainali ◽  
William J. O‘Brien ◽  
Witold Karol Subczynski
Keyword(s):  
Plasma Membrane ◽  
Protein Interactions ◽  
Fiber Cell ◽  
Cell Plasma Membrane ◽  
Cell Plasma ◽  
Lipid Protein Interactions

scholarly journals Lipid–protein interactions in virus assembly and budding from the host cell plasma membrane

2021 ◽  
Author(s):  
Balindile B. Motsa ◽  
Robert V. Stahelin
Keyword(s):  
Plasma Membrane ◽  
Host Cell ◽  
Lipid Bilayer ◽  
Protein Interactions ◽  
Current Knowledge ◽  
Virus Assembly ◽  
Cell Plasma Membrane ◽  
New Host ◽  
Cell Plasma ◽  
Lipid Protein Interactions

Lipid enveloped viruses contain a lipid bilayer coat that protects their genome to help facilitate entry into the new host cell. This lipid bilayer comes from the host cell which they infect. After viral replication, the mature virion hijacks the host cell plasma membrane where it is then released to infect new cells. This process is facilitated by the interaction between phospholipids that make up the plasma membrane and specialized viral matrix proteins. This step in the viral lifecycle may represent a viable therapeutic strategy for small molecules that aim to block enveloped virus spread. In this review, we summarize the current knowledge on the role of plasma membrane lipid–protein interactions on viral assembly and budding.

Membrane Protein Mobility and Orientation Preserved in Supported Bilayers Created Directly from Cell Plasma Membrane Blebs

Langmuir ◽  
2016 ◽  
Vol 32 (12) ◽  
pp. 2963-2974 ◽  
Author(s):  
Mark J. Richards ◽  
Chih-Yun Hsia ◽  
Rohit R. Singh ◽  
Huma Haider ◽  
Julia Kumpf ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Protein ◽  
Cell Plasma Membrane ◽  
Supported Bilayers ◽  
Protein Mobility ◽  
Cell Plasma ◽  
Membrane Blebs

Membrane Protein–Lipid Interactions Probed Using Mass Spectrometry

2019 ◽  
Vol 88 (1) ◽  
pp. 85-111 ◽  
Author(s):  
Jani Reddy Bolla ◽  
Mark T. Agasid ◽  
Shahid Mehmood ◽  
Carol V. Robinson
Keyword(s):  
Mass Spectrometry ◽  
Membrane Protein ◽  
Lipid Bilayers ◽  
Protein Interactions ◽  
Native Mass Spectrometry ◽  
Lipid Interactions ◽  
X Ray Crystallography ◽  
Native Ms ◽  
Molecular Aspects ◽  
Lipid Protein Interactions

Membrane proteins that exist in lipid bilayers are not isolated molecular entities. The lipid molecules that surround them play crucial roles in maintaining their full structural and functional integrity. Research directed at investigating these critical lipid–protein interactions is developing rapidly. Advancements in both instrumentation and software, as well as in key biophysical and biochemical techniques, are accelerating the field. In this review, we provide a brief outline of structural techniques used to probe protein–lipid interactions and focus on the molecular aspects of these interactions obtained from native mass spectrometry (native MS). We highlight examples in which lipids have been shown to modulate membrane protein structure and show how native MS has emerged as a complementary technique to X-ray crystallography and cryo–electron microscopy. We conclude with a short perspective on future developments that aim to better understand protein–lipid interactions in the native environment.

scholarly journals Temporal resolution of protein–protein interactions in the live-cell plasma membrane

2010 ◽  
Vol 397 (8) ◽  
pp. 3339-3347 ◽  
Author(s):  
Julian Weghuber ◽  
Stefan Sunzenauer ◽  
Birgit Plochberger ◽  
Mario Brameshuber ◽  
Thomas Haselgrübler ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Temporal Resolution ◽  
Protein Interactions ◽  
Live Cell ◽  
Protein Protein Interactions ◽  
Cell Plasma Membrane ◽  
Cell Plasma
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