Capillary electrophoresis with UV detection, on-line stacking and off-line dispersive liquid–liquid microextraction for determination of verapamil enantiomers in plasma

2015 ◽  
Vol 7 (14) ◽  
pp. 5820-5829 ◽  
Author(s):  
Samin Hamidi ◽  
Abolghasem Jouyban
Keyword(s):  
Capillary Electrophoresis ◽  
Human Plasma ◽  
Array Detector ◽  
Diode Array ◽  
Plasma Samples ◽  
Diode Array Detector ◽  
On Line ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

A rapid and reliable dispersive liquid–liquid microextraction (DLLME) method was coupled with field-amplified sample injection (FASI) in capillary electrophoresis with a diode array detector for the quantification of verapamil enantiomers in human plasma samples.

scholarly journals Development and Validation of Sibutramine Determination in Drug Products by Capillary Electrophoresis

2020 ◽  
Vol 9 (4) ◽  
pp. 141-145
Author(s):  
A. M. Sukhanova ◽  
I. B. Perova ◽  
K. I. Eller ◽  
G. M. Rodioinova ◽  
S. V. Chernova ◽  
...  
Keyword(s):  
Capillary Electrophoresis ◽  
Quantitative Determination ◽  
Limit Of Detection ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Limit Of Quantification ◽  
Drug Products ◽  
Development And Validation

Introduction. Recently, there has been a growing trend in the number of obese and overweight patients. To date, sibutramine is the most effective drug for treating obesity and overweight. The drug is an inhibitor of the reuptake of serotonin and norepinephrine, which leads to a decrease in hunger, and therefore, to weight loss.Aim. To develop and validate a methodology for the determination of sibutramine in drugs by capillary electrophoresis (CE) using an ultraviolet diode array detector.Materials and methods. Quantitative determination of sibutramine in drugs was carried out using the CE method with an ultraviolet diode array detector. A solution of phosphate buffer 50 mmol pH = 7.0 was used as a solvent and working electrolyte; to separate the peaks – quartz capillary 56 cm, 50 μm.Results and discussion. The developed method was validated according to the following parameters: specificity, linearity, correctness, precision, limit of detection and limit of quantification.Conclusion. A method for the quantitative determination of sibutramine in drugs by the CE method using an ultraviolet diode array detector has been developed and validated. This method meets all the requirements of General Pharmacopoeia Monograph 1.1.0012.15 «Validation of the analytical method» and can be used to control the quality of drugs, the active pharmaceutical substance of which is sibutramine.

Ultrasound-Assisted Dispersive Liquid–Liquid Microextraction and Capillary Electrophoretic Determination of Tramadol in Human Plasma

2020 ◽  
Vol 16 ◽  
Author(s):  
Paria Habibollahi ◽  
Azam Samadi ◽  
Alireza Garjani ◽  
Samad Shams Vahdati ◽  
Hamid-Reza Sargazi ◽  
...  
Keyword(s):  
Human Plasma ◽  
Limit Of Detection ◽  
Plasma Samples ◽  
Extraction Solvent ◽  
Human Plasma Samples ◽  
Good Repeatability ◽  
Ultrasound Assisted ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

BACKGROUND: Tramadol, (±)-trans-2-[(dimethylamino) methyl]-1-(3-methoxyphenyl) cyclohexanol, is a synthetic centrally acting analgesic used in the treatment of moderate to chronic pain. Tramadol like other narcotic drugs is used for the treatment of pain and also may be abused. Its overdose can cause adverse effects such as dizziness, vomiting, and nausea. The aim of this paper is to develop a sample preparation method for the determination of tramadol in human plasma samples followed by CE analysis. METHODS: Ultrasound assisted-dispersive liquid–liquid microextraction using binary mixed extractant solvent (chloroform and ethyl acetate) was used for extraction of one hundred microliters of tramadol spiked human plasma samples and in real human plasma samples obtained from the patients with abuse of tramadol. After evaporation the extractant solvent, the residue was reconstituted in 100 µL deionized water and subsequently analyzed by CE-UV. RESULTS: The developed method has remarkable characteristics including simplicity, good repeatability and appreciable accuracy. Under the best extraction conditions, low limit of detection at 7.0 µg per liter level with good linearity in the range of 0.02–10 µg mL‒1 was obtained. CONCLUSION: UA-DLLME using a binary mixed extraction solvent was established for the determination of tramadol in human plasma samples via CE method with UV-detection. In addition, the analysis of tramadol in some plasma samples of patients with abuse of tramadol indicated that the method has acceptable performance for determination of tramadol in plasma samples which indicate that the method is suitable for clinical applications.

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