Egg shell membrane – a potential natural scaffold for human meniscal tissue engineering: an in vitro study

RSC Advances ◽  
2015 ◽  
Vol 5 (93) ◽  
pp. 76019-76025 ◽  
Author(s):  
Mamatha M. Pillai ◽  
T. R. Akshaya ◽  
V. Elakkiya ◽  
J. Gopinathan ◽  
K. Santosh Sahanand ◽  
...  

Enhanced human primary meniscal cell proliferation in autoclaved egg shell membrane.

2021 ◽  
Vol 11 (9) ◽  
pp. 1792-1798
Author(s):  
Li Yan ◽  
Ge Jingping ◽  
Yin Yuanyuan ◽  
Li Xiaomei ◽  
Zhao Boxiang ◽  
...  

Aim: This research was to investigate the effects and mechanisms of HSYA in vascular endothelial injury by vitro study. Methods: Dividing HUVECs as Normal Control (NC), Model (LPS treated) group, HSYA-L, HSYA-M and HSYA-H groups. Cells in the HSYA treatment groups were treated with LPS, followed by 40 mg/ml, 80 mg/ml, and 120 mg/ml HSYA intervention (HSYA-L, HSYA-M, and HSYA -H groups), respectively. Measuring the cell proliferation, apoptosis, relative proteins and mRNA (TLR4, MyD88 and NF-κB(p65)) expressions by MTT, Flow cytometry, WB and RT-qPCR assay. Using cellular immunofluorescence to evaluate NF-κB(p65) nuclear volume of difference groups. Results: With HSYA supplement, the cell proliferation rates were significantly up-regulation with cell apoptosis significantly down-regulation with TLR4 relatived mRNA and proteins and NF-κB(p65) nuclear significantly depressed with dose-dependent (P <0.05, respectively). Conclusion: HSYA improved vascular endothelial injury induced by LPS via TLR4 pathway In Vitro.


2015 ◽  
Vol 11 (1) ◽  
pp. 204
Author(s):  
Nithya Jagannathan ◽  
AbilashaRamasubra manian ◽  
Pratibha Ramani ◽  
Priya Premkumar ◽  
Anuja Natesan ◽  
...  

2012 ◽  
Vol 70 (3) ◽  
pp. 647-656 ◽  
Author(s):  
Basel Sharaf ◽  
Caroline B. Faris ◽  
Harutsugi Abukawa ◽  
Srinivas M. Susarla ◽  
Joseph P. Vacanti ◽  
...  

2009 ◽  
Vol 189 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Susana Moreira ◽  
Naisandra Bezerra Silva ◽  
Jailma Almeida-Lima ◽  
Hugo Alexandre Oliveira Rocha ◽  
Silvia Regina Batistuzzo Medeiros ◽  
...  

1991 ◽  
Vol 62 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Sven-Olof Abrahamsson ◽  
L. Stefan Lohmander ◽  
Göran Lundborg

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