Hydroxyl Safflower Yellow Pigment A (HSYA) Improves Vascular Endothelial Injury Induced by Lipopolysaccharide In Vitro Study

2021 ◽  
Vol 11 (9) ◽  
pp. 1792-1798
Author(s):  
Li Yan ◽  
Ge Jingping ◽  
Yin Yuanyuan ◽  
Li Xiaomei ◽  
Zhao Boxiang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Treated Group ◽  
Yellow Pigment ◽  
Endothelial Injury ◽  
Vitro Study ◽  
Vascular Endothelial ◽  
Treatment Groups ◽  
Dose Dependent

Aim: This research was to investigate the effects and mechanisms of HSYA in vascular endothelial injury by vitro study. Methods: Dividing HUVECs as Normal Control (NC), Model (LPS treated) group, HSYA-L, HSYA-M and HSYA-H groups. Cells in the HSYA treatment groups were treated with LPS, followed by 40 mg/ml, 80 mg/ml, and 120 mg/ml HSYA intervention (HSYA-L, HSYA-M, and HSYA -H groups), respectively. Measuring the cell proliferation, apoptosis, relative proteins and mRNA (TLR4, MyD88 and NF-κB(p65)) expressions by MTT, Flow cytometry, WB and RT-qPCR assay. Using cellular immunofluorescence to evaluate NF-κB(p65) nuclear volume of difference groups. Results: With HSYA supplement, the cell proliferation rates were significantly up-regulation with cell apoptosis significantly down-regulation with TLR4 relatived mRNA and proteins and NF-κB(p65) nuclear significantly depressed with dose-dependent (P <0.05, respectively). Conclusion: HSYA improved vascular endothelial injury induced by LPS via TLR4 pathway In Vitro.

scholarly journals Alleviation of Hyperglycemia Induced Vascular Endothelial Injury by Exenatide Might Be Related to the Reduction of Nitrooxidative Stress

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Qian Zhao ◽  
Chun-ling Xu ◽  
Hai-yan Xiong ◽  
Wen Huang ◽  
Mei Zhang ◽  
...  
Keyword(s):  
High Glucose ◽  
Low Dose ◽  
Protective Action ◽  
In Vitro Study ◽  
Endothelial Injury ◽  
Vitro Study ◽  
Control Group ◽  
High Dose ◽  
Vascular Endothelial

We will investigate the effects of exenatide on vascular endothelial injury and nitrooxidative stress in hyperglycemia both in vivo and in vitro and explore the role of nitrooxidative stress in endothelium-protective action of exenatide. Healthy male Wistar rats were randomly divided into 4 groups: control, diabetes mellitus (DM) model, low dose of exenatide treatment, and high dose of exenatide treatment. In vitro study showed that, compared with control group, the DM rats exhibited a lowered endothelium-dependent relaxation and damaged structural integrity of thoracic aortas, and there was a significant increase in plasma nitrotyrosine concentration. These parameters were improved after treatment with either low dose or high dose of exenatide for 45 days. In vitro study showed that exendin-4 (the active ingredient of exenatide) attenuated HUVECs injury induced by high glucose, with improving cell viability and attenuating cell apoptosis. Exendin-4 also significantly alleviated the increased malondialdehyde (MDA), nitrotyrosine content, and inducible nitric oxide synthase (iNOS) expression induced by high glucose in HUVECs. In conclusion, this study demonstrates that exenatide treatment can alleviate the vascular endothelial injury, as well as attenuating the nitrooxidative stress in hyperglycemia, implying that the endothelium-protective effect of exenatide might be related to the reduction of nitrooxidative stress.

Acute phase injury of vascular endothelial cells under extra-cellular talaporfin sodium existence: In vitro study

2015 ◽  
Vol 12 (3) ◽  
pp. 351
Author(s):  
Risa Hamada ◽  
Ryota Matsuzaki ◽  
Hiromi Takenoya ◽  
Mariko Kurotsu ◽  
Emiyu Ogawa ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Acute Phase ◽  
Vascular Endothelial Cells ◽  
In Vitro Study ◽  
Vitro Study ◽  
Vascular Endothelial ◽  
Talaporfin Sodium

scholarly journals BC nanofibres: In vitro study of genotoxicity and cell proliferation

2009 ◽  
Vol 189 (3) ◽  
pp. 235-241 ◽  
Author(s):  
Susana Moreira ◽  
Naisandra Bezerra Silva ◽  
Jailma Almeida-Lima ◽  
Hugo Alexandre Oliveira Rocha ◽  
Silvia Regina Batistuzzo Medeiros ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study

Dehydration inhibits matrix synthesis and cell proliferation: An in vitro study of rabbit flexor tendons

1991 ◽  
Vol 62 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Sven-Olof Abrahamsson ◽  
L. Stefan Lohmander ◽  
Göran Lundborg
Keyword(s):  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Matrix Synthesis ◽  
Flexor Tendons

Evaluation of Osteoblast Responses to Silver-Hydroxyapatite/Titania Nanoparticles In Vitro

2007 ◽  
Vol 330-332 ◽  
pp. 447-450 ◽  
Author(s):  
Jing Chao Zhang ◽  
Juan Liao ◽  
An Chun Mo ◽  
Hong Kun Wu ◽  
Yu Bao Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Proliferation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Titania Nanoparticles ◽  
Respiration Rates ◽  
The Difference

In the present in vitro study, osteoblasts proliferation, vitality and ultrastructure were investigated when cultured in the presence of silver-hydroxyapatite/titania nanoparticles (nAg_HA/TiO2) compared to HA nanoparticles (nHA) at various concentrations and cell culture without nanoparticles for up to 120 hours. Results confirmed the detrimental influences of both nAg_HA/TiO2 and nHA on osteoblast growth.Cell vitality was slightly higher during the earlier 24h, but after that was inhibited. Both cell proliferation and vitality by addition of nanoparticles were restricted with concentrations of nanoparticles increasing. However, the respiration rates by addition of nanoparticles were showed higher than that of the cell culture without nanoparticles. No remarkable ultrastructure changes were showed in the osteoblasts exposed nanoparticles. The difference in cell proliferation, vitality and ultrastructure between nAg_HA/TiO2 and nHA were insignificant. It was demonstrated that biocompatibility of nAg_HA/TiO2 is almost the same as nHA.

Sign in / Sign up

Export Citation Format

Share Document