scholarly journals Engineering a nanolab for the determination of lysosomal nitric oxide by the rational design of a pH-activatable fluorescent probe

2016 ◽  
Vol 7 (3) ◽  
pp. 1920-1925 ◽  
Author(s):  
Yinhui Li ◽  
Wei Wu ◽  
Jinfeng Yang ◽  
Lin Yuan ◽  
Changhui Liu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Rational Design ◽  
Living Cells

A pH-activatable fluorescent probe, Rhod-H-NO, was designed and synthesized for the determination of lysosomal NO in living cells and in vivo.

scholarly journals Dihydropyridine-coumarin-based fluorescent probe for imaging nitric oxide in living cells

2020 ◽  
Vol 19 (9) ◽  
pp. 1230-1235
Author(s):  
Sufang Ma ◽  
Xueyi Sun ◽  
Qiang Yu ◽  
Rui Liu ◽  
Zhonglin Lu ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Fluorescent Probe ◽  
Living Cells

A new fluorescent probe for nitric oxide (NO) was prepared, which shows great potential for understanding the biological roles of NO in vivo.

Carbon dots derived from Fusobacterium nucleatum for intracellular determination of Fe3+ and bioimaging both in vitro and in vivo

2021 ◽  
Author(s):  
Lijuan Liu ◽  
Shengting Zhang ◽  
Xiaodan Zheng ◽  
Hongmei Li ◽  
Qi Chen ◽  
...  
Keyword(s):  
Carbon Dots ◽  
Fusobacterium Nucleatum ◽  
Living Cells ◽  
First Time ◽  
Fluorescent Carbon Dots ◽  
Fluorescent Carbon

Fusobacterium nucleatum has been employed for the first time to synthesize fluorescent carbon dots which could be applied for the determination of Fe3+ ions in living cells and bioimaging in vitro and in vivo with excellent biocompatibility.

A Simple Silicon Based Nitric Oxide Sensor

1999 ◽  
Author(s):  
Marcelo Bariatto ◽  
Rogerio Furlan ◽  
Koiti Arakai ◽  
Jorge J. Santiago-Aviles
Keyword(s):  
Nitric Oxide ◽  
Amperometric Detection ◽  
Cellular Level ◽  
High Yield ◽  
Sensor Calibration ◽  
Nitric Oxide Sensor ◽  
Sensor Configuration

Abstract Nitric oxide (NO) is known to mediate many beneficial physiology processes, motivating its detection in vivo as well as in vitro. Electrochemical detection provides the required cellular level determination of NO among several other techniques. In this work, electrochemical micro-sensors for both types of detection, in vivo and in vitro, were developed, exploring the silicon planar technology, which presents high yield and reliability and also permits batch fabrication. The developed in vitro sensor features eight detection sites (10 μm × 10 μm microelectrodes), for determination of nitric oxide spatial distribution or multi-species analysis. Different electrochemical methods were applied to provide sensor calibration and chemical reproducibility. For in vivo analysis, the designed structures have a needle shape (40 μm thick) and they were silicon micro-machined by using plasma etching or etch stop techniques. Different configurations were designed and implemented, containing a number of detection microelectrodes that vary from 2 to 10. The amperometric detection of both nitric oxide and nitride (NO2−) — a molecule that causes an interference — were investigated by using the in vitro micro-sensor configuration. The need of a cationic exchanger (Nafion) was demonstrated in order to provide selectivity to NO for low concentrations. Also, the developed sensor has a sensitivity of 500 A/M.cm2 and a detection limit of 10 μM.

A lysosome targetable fluorescent probe for endogenous imaging of hydrogen peroxide in living cells

2017 ◽  
Vol 53 (26) ◽  
pp. 3701-3704 ◽  
Author(s):  
Shahi Imam Reja ◽  
Muskan Gupta ◽  
Neha Gupta ◽  
Vandana Bhalla ◽  
Puja Ohri ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Fluorescent Probe ◽  
Living Cells

A lysosome targetable naphthalimide based fluorescent probe has been designed and synthesized for selective imaging of H2O2 in different cells, tissues and in vivo models.

A novel ratiometric AIE-based fluorescent probe for specific detection of Hcy/Cys and imaging of living cells in vivo

2021 ◽  
Author(s):  
Hong-Min Lv ◽  
Zi-Ke Jiang ◽  
Jing Wang ◽  
Tao Wang ◽  
Xiao-Nan Zhang ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Condensation Reaction ◽  
Living Cells ◽  
Specific Detection ◽  
Sensing Mechanism

The sensing mechanism toward Hcy/Cys is realized based on the condensation reaction, which breaks CN to form a thiazolidine adduct.

The development of a hemicyanine-based ratiometric CO fluorescent probe with a long emission wavelength and its applications for imaging CO in vitro and in vivo

2020 ◽  
Vol 44 (28) ◽  
pp. 12107-12112 ◽  
Author(s):  
Yunyan Zhang ◽  
Xiuqi Kong ◽  
Yonghe Tang ◽  
Min Li ◽  
Yaguang Yin ◽  
...  
Keyword(s):  
Fluorescent Probe ◽  
Living Cells ◽  
Emission Wavelength ◽  
Long Wavelength ◽  
Wavelength Emission ◽  
Ratiometric Fluorescent Probe

A novel ratiometric fluorescent probe, Hcy-CO, with long-wavelength emission was developed for visualizing CO in living cells and zebrafish.

Rational Design of a Reversible Fluorescent Probe for Sensing Sulfur Dioxide/Formaldehyde in Living Cells, Zebrafish, and Living Mice

2019 ◽  
Vol 91 (16) ◽  
pp. 10723-10730 ◽  
Author(s):  
Yanyan Ma ◽  
Yonghe Tang ◽  
Yuping Zhao ◽  
Weiying Lin
Keyword(s):  
Sulfur Dioxide ◽  
Fluorescent Probe ◽  
Rational Design ◽  
Living Cells

Rational design of stable near-infrared cyanine-based probe with remarkable large Stokes Shift for monitoring Carbon monoxide in living cells and in vivo

2019 ◽  
Vol 171 ◽  
pp. 107753 ◽  
Author(s):  
Wenda Zhang ◽  
Yu Wang ◽  
Junqiang Dong ◽  
Yonggao Zhang ◽  
Jiawen Zhu ◽  
...  
Keyword(s):  
Carbon Monoxide ◽  
Rational Design ◽  
Near Infrared ◽  
Stokes Shift ◽  
Living Cells ◽  
Large Stokes Shift
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