Nuclear stiffening and chromatin softening with progerin expression leads to an attenuated nuclear response to force

Progerin, a mutant form of the nuclear protein lamin A, is associated with the premature aging disorder Hutchinson-Gilford progeria syndrome. Progerin expression leads to a variety of changes in nuclear structure, mechanics and mechano-responsiveness.

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Towards a Drosophila model of Hutchinson–Gilford progeria syndrome

Biochemical Society Transactions ◽

10.1042/bst0361389 ◽

2008 ◽

Vol 36 (6) ◽

pp. 1389-1392 ◽

Cited By ~ 6

Author(s):

Gemma S. Beard ◽

Joanna M. Bridger ◽

Ian R. Kill ◽

David R.P. Tree

Keyword(s):

Drosophila Melanogaster ◽

Premature Aging ◽

Lamin A ◽

Wild Type ◽

Drosophila Model ◽

Numerous Cell ◽

Cellular Abnormalities ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome ◽

Adult Drosophila

The laminopathy Hutchinson–Gilford progeria syndrome (HGPS) is caused by the mutant lamin A protein progerin and leads to premature aging of affected children. Despite numerous cell biological and biochemical insights into the basis for the cellular abnormalities seen in HGPS, the mechanism linking progerin to the organismal phenotype is not fully understood. To begin to address the mechanism behind HGPS using Drosophila melanogaster, we have ectopically expressed progerin and lamin A. We found that ectopic progerin and lamin A phenocopy several effects of laminopathies in developing and adult Drosophila, but that progerin causes a stronger phenotype than wild-type lamin A.

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A pathway linking oxidative stress and the Ran GTPase system in progeria

Molecular Biology of the Cell ◽

10.1091/mbc.e13-07-0430 ◽

2014 ◽

Vol 25 (8) ◽

pp. 1202-1215 ◽

Cited By ~ 26

Author(s):

Sutirtha Datta ◽

Chelsi J. Snow ◽

Bryce M. Paschal

Keyword(s):

Oxidative Stress ◽

Nuclear Membrane ◽

Nucleocytoplasmic Transport ◽

Lamin A ◽

Mutant Form ◽

Ran Gtpase ◽

Nuclear Levels ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome ◽

The Relationship

Maintaining the Ran GTPase at a proper concentration in the nucleus is important for nucleocytoplasmic transport. Previously we found that nuclear levels of Ran are reduced in cells from patients with Hutchinson–Gilford progeria syndrome (HGPS), a disease caused by constitutive attachment of a mutant form of lamin A (termed progerin) to the nuclear membrane. Here we explore the relationship between progerin, the Ran GTPase, and oxidative stress. Stable attachment of progerin to the nuclear membrane disrupts the Ran gradient and results in cytoplasmic localization of Ubc9, a Ran-dependent import cargo. Ran and Ubc9 disruption can be induced reversibly with H2O2. CHO cells preadapted to oxidative stress resist the effects of progerin on Ran and Ubc9. Given that HGPS-patient fibroblasts display elevated ROS, these data suggest that progerin inhibits nuclear transport via oxidative stress. A drug that inhibits pre–lamin A cleavage mimics the effects of progerin by disrupting the Ran gradient, but the effects on Ran are observed before a substantial ROS increase. Moreover, reducing the nuclear concentration of Ran is sufficient to induce ROS irrespective of progerin. We speculate that oxidative stress caused by progerin may occur upstream or downstream of Ran, depending on the cell type and physiological setting.

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Neuropeptide Y Enhances Progerin Clearance and Ameliorates the Senescent Phenotype of Human Hutchinson-Gilford Progeria Syndrome Cells

The Journals of Gerontology Series A ◽

10.1093/gerona/glz280 ◽

2020 ◽

Vol 75 (6) ◽

pp. 1073-1078 ◽

Cited By ~ 3

Author(s):

Célia A Aveleira ◽

Marisa Ferreira-Marques ◽

Luísa Cortes ◽

Jorge Valero ◽

Dina Pereira ◽

...

Keyword(s):

Neuropeptide Y ◽

Caloric Restriction ◽

Cellular Senescence ◽

Dermal Fibroblasts ◽

Cellular Aging ◽

Premature Aging ◽

Whole Body ◽

Lamin A ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome

Abstract Hutchinson-Gilford progeria syndrome (HGPS, or classical progeria) is a rare genetic disorder, characterized by premature aging, and caused by a de novo point mutation (C608G) within the lamin A/C gene (LMNA), producing an abnormal lamin A protein, termed progerin. Accumulation of progerin causes nuclear abnormalities and cell cycle arrest ultimately leading to cellular senescence. Autophagy impairment is a hallmark of cellular aging, and the rescue of this proteostasis mechanism delays aging progression in HGPS cells. We have previously shown that the endogenous Neuropeptide Y (NPY) increases autophagy in hypothalamus, a brain area already identified as a central regulator of whole-body aging. We also showed that NPY mediates caloric restriction-induced autophagy. These results are in accordance with other studies suggesting that NPY may act as a caloric restriction mimetic and plays a role as a lifespan and aging regulator. The aim of the present study was, therefore, to investigate if NPY could delay HGPS premature aging phenotype. Herein, we report that NPY increases autophagic flux and progerin clearance in primary cultures of human dermal fibroblasts from HGPS patients. NPY also rescues nuclear morphology and decreases the number of dysmorphic nuclei, a hallmark of HGPS cells. In addition, NPY decreases other hallmarks of aging as DNA damage and cellular senescence. Altogether, these results show that NPY rescues several hallmarks of cellular aging in HGPS cells, suggesting that NPY can be considered a promising strategy to delay or block the premature aging of HGPS.

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Hutchinson–Gilford Progeria Syndrome (Hgps) And Application Of Gene Therapy Based Crispr/Cas Technology As A Promising Innovative Treatment Approach

Recent Patents on Biotechnology ◽

10.2174/1872208315666210928114720 ◽

2021 ◽

Vol 15 ◽

Author(s):

Mekha Rajeev ◽

Chameli Ratan ◽

Karthik Krishnan ◽

Meenu Vijayan

Keyword(s):

De Novo ◽

Treatment Approach ◽

Genetic Disorder ◽

Symptomatic Relief ◽

Dominant Negative ◽

Premature Aging ◽

Lamin A ◽

Promising Therapeutic Approach ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome

Background: Hutchinson–Gilford progeria syndrome (HGPS) also known as progeria of childhood or progeria is a rare, rapid, autosomal dominant genetic disorder characterized by premature aging which occurs shortly after birth. HGPS occurs as a result of de novo point mutation in the gene recognized as LMNA gene that encodes two proteins Lamin A protein and Lamin C protein which are the structural components of the nuclear envelope. Mutations in the gene trigger abnormal splicing and induce internal deletion of 50 amino acids leading to the development of a truncated form of Lamin A protein known as Progerin. Progerin generation can be considered as the crucial step in HGPS since the protein is highly toxic to human cells, permanently farnesylated, and exhibits variation in several biochemical and structural properties within the individual. HGPS also produces complications such as skin alterations, growth failure, atherosclerosis, hair and fat loss, and bone and joint diseases. We have also revised all relevant patents relating to Hutchinson-gilford progeria syndrome and its therapy in the current article. Method: The goal of the present review article is to provide information about Hutchinson–Gilford progeria syndrome (HGPS) and the use of CRISPR/Cas technology as a promising treatment approach in the treatment of the disease. The review also discusses about different pharmacological and non-pharmacological methods of treatment currently used for HGPS. Results : The main limitation associated with progeria is the lack of a definitive cure. The existing treatment modality provides only symptomatic relief. Therefore, it is high time to develop a therapeutic method that hastens premature aging in such patients. Conclusion: CRISPR/Cas technology is a novel gene-editing tool that allows genome editing at specific loci, and is found to be a promising therapeutic approach for the treatment of genetic disorders such as HGPS where dominant-negative mutations take place.

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Emerging candidate treatment strategies for Hutchinson-Gilford progeria syndrome

Biochemical Society Transactions ◽

10.1042/bst20170141 ◽

2017 ◽

Vol 45 (6) ◽

pp. 1279-1293 ◽

Cited By ~ 6

Author(s):

Charlotte Strandgren ◽

Gwladys Revêchon ◽

Agustín Sola Carvajal ◽

Maria Eriksson

Keyword(s):

De Novo ◽

Disease Incidence ◽

Treatment Strategies ◽

Premature Aging ◽

Lamin A ◽

Lmna Gene ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome

Hutchinson-Gilford progeria syndrome (HGPS, progeria) is an extremely rare premature aging disorder affecting children, with a disease incidence of ∼1 in 18 million individuals. HGPS is usually caused by a de novo point mutation in exon 11 of the LMNA gene (c.1824C>T, p.G608G), resulting in the increased usage of a cryptic splice site and production of a truncated unprocessed lamin A protein named progerin. Since the genetic cause for HGPS was published in 2003, numerous potential treatment options have rapidly emerged. Strategies to interfere with the post-translational processing of lamin A, to enhance progerin clearance, or directly target the HGPS mutation to reduce the progerin-producing alternative splicing of the LMNA gene have been developed. Here, we give an up-to-date resume of the contributions made by our and other research groups to the growing list of different candidate treatment strategies that have been tested, both in vitro, in vivo in mouse models for HGPS and in clinical trials in HGPS patients.

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Importance of molecular cell biology investigations in human medicine in the story of the Hutchinson-Gilford progeria syndrome

Interdisciplinary Toxicology ◽

10.2478/v10102-010-0018-y ◽

2010 ◽

Vol 3 (3) ◽

pp. 89-93 ◽

Cited By ~ 1

Author(s):

Ivan Raška

Keyword(s):

Cell Biology ◽

Basic Mechanism ◽

Human Medicine ◽

Premature Aging ◽

Lamin A ◽

Nucleotide Position ◽

Molecular Cell Biology ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome ◽

Precursor Mrna

Importance of molecular cell biology investigations in human medicine in the story of the Hutchinson-Gilford progeria syndromeRanged among laminopathies, Hutchinson-Gilford progeria syndrome is a syndrome that involves premature aging, leading usually to death at the age between 10 to 14 years predominatly due to a myocardial infarction or a stroke. In the lecture I shall overview the importance of molecular cell biology investigations that led to the discovery of the basic mechanism standing behind this rare syndrome. The genetic basis in most cases is a mutation at the nucleotide position 1824 of the lamin A gene. At this position, cytosine is substituted for thymine so that a cryptic splice site within the precursor mRNA for lamin A is generated. This results in a production of abnormal lamin A, termed progerin, its presence in cells having a deleterious dominant effect. Depending on the cell type and tissue, progerin induces a pleiotropy of defects that vary in different tissues. The present endeavour how to challenge this terrible disease will be also mentioned.

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DNA-damage accumulation and replicative arrest in Hutchinson–Gilford progeria syndrome

Biochemical Society Transactions ◽

10.1042/bst20110687 ◽

2011 ◽

Vol 39 (6) ◽

pp. 1764-1769 ◽

Cited By ~ 46

Author(s):

Phillip R. Musich ◽

Yue Zou

Keyword(s):

Dna Damage ◽

Cell Cycle Progression ◽

Severe Form ◽

Premature Aging ◽

Lamin A ◽

Repair System ◽

Group A ◽

Fork Stalling ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome

A common feature of progeria syndromes is a premature aging phenotype and an enhanced accumulation of DNA damage arising from a compromised repair system. HGPS (Hutchinson–Gilford progeria syndrome) is a severe form of progeria in which patients accumulate progerin, a mutant lamin A protein derived from a splicing variant of the lamin A/C gene (LMNA). Progerin causes chromatin perturbations which result in the formation of DSBs (double-strand breaks) and abnormal DDR (DNA-damage response). In the present article, we review recent findings which resolve some mechanistic details of how progerin may disrupt DDR pathways in HGPS cells. We propose that progerin accumulation results in disruption of functions of some replication and repair factors, causing the mislocalization of XPA (xeroderma pigmentosum group A) protein to the replication forks, replication fork stalling and, subsequently, DNA DSBs. The binding of XPA to the stalled forks excludes normal binding by repair proteins, leading to DSB accumulation, which activates ATM (ataxia telangiectasia mutated) and ATR (ATM- and Rad3-related) checkpoints, and arresting cell-cycle progression.

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Hutchinson-Gilford Progeria Syndrome: An Overview of the Molecular Mechanism, Pathophysiology and Therapeutic Approach

Current Gene Therapy ◽

10.2174/1566523221666210303100805 ◽

2021 ◽

Vol 21 ◽

Author(s):

Md. Mominur Rahman ◽

Kazi Sayma Ferdous ◽

Muniruddin Ahmed ◽

Mohammad Touhidul Islam ◽

Md. Robin Khan ◽

...

Keyword(s):

Molecular Mechanism ◽

Nuclear Structure ◽

Early Stage ◽

Treatment Options ◽

Lamin A ◽

Lmna Gene ◽

Inherited Disorders ◽

Appropriate Treatment ◽

Progeria Syndrome ◽

Hutchinson Gilford Progeria Syndrome

: Lamin A/C encoded by LMNA gene is an important component for the maintenance of the nuclear structure. Mutation in the lamin A/C leads to a group of inherited disorders is known as laminopathies. In the human body, there are several mutations in the LMNA gene have been identified. It can affect diverse organs or tissues or can be systemic, causing different diseases. In this review, we mainly focused on one of the most severe laminopathies, Hutchinson-Gilford progeria syndrome (HGPS). HGPS is an immensely uncommon, deadly, metameric ill-timed laminopathies caused by the abnormal splicing of the LMNA gene and production of an aberrant protein known as progerin. Here, we also presented the currently available data on the molecular mechanism, pathophysiology, available treatment, and future approaches of this deadly disease. Due to the production of progerin an abnormal protein leads to an abnormality in nuclear structure, defects in DNA repair, shortening of telomere, impairment in gene regulation which ultimately results in aging in the early stage of life. Now some treatment options are available for this disease but a proper understanding of the molecular mechanism of this disease will help to develop a more appropriate treatment which makes it an emerging area of research.

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