scholarly journals An electrochemical biosensor for rapid detection of E. coli O157:H7 with highly efficient bi-functional glucose oxidase-polydopamine nanocomposites and Prussian blue modified screen-printed interdigitated electrodes

The Analyst ◽  
2016 ◽  
Vol 141 (18) ◽  
pp. 5441-5449 ◽  
Author(s):  
Meng Xu ◽  
Ronghui Wang ◽  
Yanbin Li
Keyword(s):  
Prussian Blue ◽  
Glucose Oxidase ◽  
Rapid Detection ◽  
Electrochemical Biosensor ◽  
Interdigitated Electrodes ◽  
E Coli ◽  
Highly Efficient ◽  
Screen Printed

An electrochemical biosensor was developed based on the bifunctional ABs/GOxext/AuNPs/MBs-GOx@PDA magnetic PMNCs that can rapidly and sensitively detect E. coli O157:H7.

scholarly journals A Label-Free Impedance Immunosensor Using Screen-Printed Interdigitated Electrodes and Magnetic Nanobeads for the Detection of E. coli O157:H7

Biosensors ◽  
2015 ◽  
Vol 5 (4) ◽  
pp. 791-803 ◽  
Author(s):  
Ronghui Wang ◽  
Jacob Lum ◽  
Zach Callaway ◽  
Jianhan Lin ◽  
Walter Bottje ◽  
...  
Keyword(s):  
Label Free ◽  
Interdigitated Electrodes ◽  
E Coli ◽  
Magnetic Nanobeads ◽  
Impedance Immunosensor ◽  
Screen Printed

COMPOSITE GLUCOSE BIOSENSOR BASED ON SCREEN-PRINTED ELECTRODES BULK MODIFIED WITH PRUSSIAN BLUE AND GLUCOSE OXIDASE

2002 ◽  
Vol 35 (6) ◽  
pp. 959-970 ◽  
Author(s):  
Miloslav Pravda ◽  
Maryann P. O'Halloran ◽  
Mark P. Kreuzer ◽  
George G. Guilbault
Keyword(s):  
Prussian Blue ◽  
Glucose Oxidase ◽  
Glucose Biosensor ◽  
Screen Printed Electrodes ◽  
Screen Printed

Study on electrochemical biosensor based on screen-printed electrode

2018 ◽  
Vol 32 (34n36) ◽  
pp. 1840061
Author(s):  
Quan Zou ◽  
Gong Cheng ◽  
Yu Zhang
Keyword(s):  
Glucose Oxidase ◽  
Redox Reaction ◽  
Electrochemical Biosensor ◽  
Scanning Speed ◽  
Potassium Ferrocyanide ◽  
Screen Printed Electrode ◽  
Three Solutions ◽  
The Relationship ◽  
Screen Printed

It is known that redox reaction can take place among the solutions of potassium ferrocyanide (K4[Fe(CN)6]), glucose (C6H[Formula: see text]O6) and glucose oxidase (Glucose Oxidase, GOD). In this work, the method of electrochemical biosensor detection based on screen printed electrode was used to observe the redox reaction among these solutions. The relationship between redox reaction and parameters was studied by examining the effects of concentration and scanning speed of three solutions.

scholarly journals Screen Printed Based Impedimetric Immunosensor for Rapid Detection of Escherichia coli in Drinking Water

Sensors ◽  
2020 ◽  
Vol 20 (1) ◽  
pp. 274 ◽  
Author(s):  
Martina Cimafonte ◽  
Andrea Fulgione ◽  
Rosa Gaglione ◽  
Marina Papaianni ◽  
Rosanna Capparelli ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Drinking Water ◽  
Rapid Detection ◽  
Electrochemical Impedance ◽  
Limit Of Detection ◽  
Phosphate Buffer Solution ◽  
Relevant Parameter ◽  
Transfer Resistance ◽  
E Coli ◽  
Screen Printed

The development of a simple and low cost electrochemical impedance immunosensor based on screen printed gold electrode for rapid detection of Escherichia coli in water is reported. The immunosensor is fabricated by immobilizing anti-E. coli antibodies onto a gold surface in a covalent way by the photochemical immobilization technique, a simple procedure able to bind antibodies upright onto gold surfaces. Impedance spectra are recorded in 0.01 M phosphate buffer solution (PBS) containing 10 mM Fe(CN)63−/Fe(CN)64− as redox probe. The Nyquist plots can be modelled with a modified Randles circuit, identifying the charge transfer resistance Rct as the relevant parameter after the immobilization of antibodies, the blocking with BSA and the binding of E. coli. The introduction of a standard amplification procedure leads to a significant enhancement of the impedance increase, which allows one to measure E. coli in drinking water with a limit of detection of 3 × 101 CFU mL−1 while preserving the rapidity of the method that requires only 1 h to provide a “yes/no” response. Additionally, by applying the Langmuir adsorption model, we are able to describe the change of Rct in terms of the “effective” electrode, which is modified by the detection of the analyte whose microscopic conducting properties can be quantified.

DECONTAMINATION OF VETERINARY SURVEILLANCE OBJECTS BY NEW GENERATION MEDICINE

1970 ◽  
pp. 64-67
Author(s):  
М. S. Saypullaev ◽  
А. U. Koychuev ◽  
Т. B. Mirzoeva
Keyword(s):  
Staphylococcus Aureus ◽  
Inhalation Exposure ◽  
Gram Negative Bacteria ◽  
Hazard Class ◽  
E Coli ◽  
Highly Efficient ◽  
Mucous Membranes ◽  
Environmentally Safe ◽  
New Generation ◽  
And Staphylococcus Aureus

The successful conduct of disinfection measures largely depends on the availability of veterinary practice a highly efficient, environmentally safe disinfectants. In this regard, finding new highly efficient disinfectant remains relevant. Studies found that the "Polied" (OOO "Razvitie XXI Vek, Russia) can be attributed to the highly efficient and environmentally friendly means. Solutions "Polied" have a high disinfectant activity against smooth and rough surfaces in the laboratory against gram-positive, gram-negative bacteria, mycobacteria and spores of microorganisms. Studies have established that solutions should be "Polied" obezzarajivatmi E. coli (EA 1257) concentrations of 0.1% on smooth surfaces and Staphylococcus aureus concentration of 0.05% in 1 hour from the calculation of 0.25-0.3 litres/m2. Disinfection of rough test surfaces against Escherichia coli and Staphylococcus aureus occurred after treatment with 0,3% solution of 3-hour exposure, at a rate of 0.5 l/m2. It was also found that 1.0% solution "Polied" fully obezzarazhivatel test the surface of mycobacteria (PCs-5) and at double the 0.6% concentration for 24 hours. Disinfection of rough test surfaces contaminated with spores of B. cereus (PCs 96) was achieved with a 4.0% solution at twice the irrigation rate of 0.5 l/m2 at an exposure of 24 hours. Toxicity solutions of the drug "Polied" refer to "moderate" threat (hazard class 3) and low-hazard substances (4 hazard class) when applied to the skin, mucous membranes of the eyes, and inhalation exposure on the respiratory system.

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

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