Determination of cocaine and its main adulterants in seized drugs from Rio Grande do Sul, Brazil, by a Doehlert optimized LC-DAD method

2016 ◽  
Vol 8 (26) ◽  
pp. 5212-5217 ◽  
Author(s):  
M. C. A. Marcelo ◽  
T. R. Fiorentin ◽  
K. C. Mariotti ◽  
R. S. Ortiz ◽  
R. P. Limberger ◽  
...  
Keyword(s):  
Liquid Chromatography ◽  
Rio Grande ◽  
The State ◽  
Array Detector ◽  
Diode Array ◽  
Rio Grande Do Sul ◽  
Diode Array Detector

A liquid chromatography with a diode array detector (LC-DAD) method was developed through Doehlert optimization for analysis of cocaine seized in the state of Rio Grande do Sul, Brazil.

scholarly journals Determination of Patulin in Apple Juice by Liquid Chromatography

2006 ◽  
Vol 89 (1) ◽  
pp. 139-143 ◽  
Author(s):  
Maria Helena Iha ◽  
Myrna Sabino
Keyword(s):  
Liquid Chromatography ◽  
Standard Deviation ◽  
Mobile Phase ◽  
Apple Juice ◽  
Array Detector ◽  
Diode Array ◽  
Diode Array Detector ◽  
Relative Standard ◽  
Precision Study

Abstract A method was developed and validated in-house for the determination of patulin (PAT), a toxic mold metabolite, in apple juice. The sample was extracted with ethyl acetatehexane and analyzed by liquid chromatography equipped with a C18 column and diode array detector. The mobile phase used for the quantification was waterethanol, at a flow rate of 0.5 mL/min. The method showed a mean recovery of 84.8%, the relative standard deviation obtained in the precision study was <7.7%, the quantification and detection limits were 7 and 3 μg/L, respectively, and the linear range for PAT in apple juice was 2.6650 μg/L. The ruggedness was evaluated by an intralaboratory experiment, in which 5 factors were studied, and only one was found to influence the observed results. The developed method is fast, practical, and simple; the solvents (except hexane) and reagents used were nontoxic. The results of the validation confirmed the efficiency of the method, which is sensitive enough to be used in studies required to quantify PAT in apple juice.

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