Hydrodynamic thermal confinement: creating thermo-chemical microenvironments on surfaces

We present a new, general concept termed Hydrodynamic Thermal Confinement (HTC), and its implementation for the creation of microscale dynamic thermo-chemical microenvironments on biological surfaces. We demonstrate selective DNA denaturation of single spots on a microarray using a 15% formamide solution at 60 °C.

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Legal regulation of the digitalization process of Ukrainian archive space

Visnyk of Kharkiv State Academy of Culture ◽

10.31516/2410-5333.060.07 ◽

2021 ◽

pp. 79-88

Author(s):

D. Vasylenko ◽

L. Butko

Keyword(s):

Legal Regulation ◽

General Concept ◽

State Regulation ◽

Digital Format ◽

Analysis And Synthesis ◽

Scientific Principles ◽

Systematic Development ◽

The Creation ◽

Theoretical Foundations ◽

State Regional

The problem statement. Archival sphere can fit organically into the general concept of digital transformation of Ukraine’s economy. The creation of digital format of archival institutions should be based on identical branch standards and rules for the creation, preserving, accounting, description, using of digital copies in order to ensure a single collaboration algorithm between state, regional and municipal authority levels. The purpose of the research is to analyze the new trends of the management system of the archival sphere from the perspective of regulatory support for the implementation of digitalization tools of Ukrainian archival institutions. The methodology. To resolve the tasks of the research were used traditional complex of common scientific principles (determinism, imaging, unity of opposites) and methods (analysis and synthesis, systematic and structural, questionnaire, content-analysis, observation, statistical). The results. The article analyzes the legal tools as an element of state regulation of digitalization of the Ukrainian archival space. The results of the study were confirmed by a sociological survey on the topic “Do we need to adjust the regulatory basis the archival branch to the digitalization contexts?”. The scientific novelty of the research is to develop the theoretical foundations in the branch of archival sphere management due to context of regulatory support for provision of digitalization issue, including methods of analysis, synthesis and systematization, to generate the problem of introducing the modernizational legislation to manage the digitalization of archival sphere. Conclusions. It is reviewed in the research the influence of the state regulation on the task of systematic development of Ukrainian branch standards and other legal documentation, which is created to regulate the requirements of creation, accounting, preserving, and use of electronic document copies, as well as the collaboration between the archival institutions with stakeholders within a single online platform.

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The features of the contractual form of protection of subjective intellectual property rights for a cinematographic work under the laws of Ukraine and the countries of the European Union

Law Review of Kyiv University of Law ◽

10.36695/2219-5521.4.2020.57 ◽

2020 ◽

pp. 323-328

Author(s):

Kateryna Poturai

Keyword(s):

European Union ◽

Intellectual Property ◽

Property Rights ◽

Intellectual Property Rights ◽

Legal Regulation ◽

General Concept ◽

The European Union ◽

Modern Development ◽

The Creation ◽

Civil Contract

The article reveals the features of the contractual form of subjective intellectual property rights to a cinematographic work underthe laws of Ukraine and the European Union as the main form of protection of cinematographic works.The author emphasizes that in modern development of copyright relations, civil law has abandoned the legislative consolidationof the concept of copyright agreement, yet only lists and discloses the sense of its types.Thus, at the legislative and theoretical level, the problem of classifying a copyright agreement on the creation of a cinematogra -phic work as an agreement on the alienation of exclusive rights or a license agreement still remains unresolved. At the present stage,the legal regulation of the creation and use of films is further complicated by the fact that many film studios instead of copyright agreementsenter into refit contracts on the creation of films.The author draws attention to the fact that the French legal doctrine does not see any difference between the subject of the contractand the content of the obligation and uses such concepts as synonyms. The French legislator does not give a general concept, whichwould reveal the main features of the copyright agreement. However, the provisions of the French Intellectual Property Code directlyindicate the possibility of assignment of the author’s property rights (succession in a certain part of copyright) on the basis of the cont -ract. In the French literature and in the practice of courts it is unanimously recognized that from the point of view of general civil lawthe assignment of exclusive property copyrights is in principle a civil contract of sale of property, in turn a license is a civil contract ofproperty lease.The author also emphasizes that there is a necessity to regulate the conclusion of copyright agreements with all subjects of a cinematographicwork, which may have intellectual property rights in connection with the creation of such a work.

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Placemaking as an approach of sustainable urban facilities management

Facilities ◽

10.1108/f-04-2020-0055 ◽

2020 ◽

Vol 38 (11/12) ◽

pp. 801-818 ◽

Cited By ~ 2

Author(s):

Milena Vukmirovic ◽

Suzana Gavrilović

Keyword(s):

Public Space ◽

Hot Spot ◽

General Concept ◽

Management Tool ◽

Facilities Management ◽

Content Type ◽

Stakeholder Identification ◽

Space Transformation ◽

The Creation ◽

Practical Implications

Purpose This paper aims to present the potential of placemaking as an approach of sustainable urban facilities management and its impacts on the improvement of the planning procedures which was aimed at involving citizens in the process itself. The study is based on the general concept of placemaking represented as an “overarching idea and a hands-on approach for improving a neighbourhood, city or region”, that serves as a process that “inspires people to collectively reimagine and reinvent public spaces as the heart of every community” (PPS, 2007). Design/methodology/approach The study used placemaking (onsite analysis, stakeholder identification, citizen survey and emotional mapping) and public participation geographic information systems (The Kernel Density tool in ArcGIS and hot spot analysis) methodologies to map problems and preferences identified by stakeholders related to particular spaces within the move formed by Maršala Birjuzova and Sremska streets in Belgrade. The research covered two-day stakeholders’ workshops including four groups of users participated in the workshop – pupils of local private high school, street residents, students of the Faculty of Forestry and the Faculty of Architecture and owners of local shops and businesses. Findings Research has shown that different stakeholders can offer very rational observations on the quality of a particular space and provide clear suggestions on its improvement and transformation. These proposals can be organised in the form of visions of the future appearance and functioning of the space, thus recognising the potential in the function of a sustainable urban facilities management tool in the form of creating a common idea, which will result in the creation of a common space. Research limitations/implications The research covered only part of the process that resulted in the creation of an idea of future public space transformation. Continued research should be conducted after the intervention, which would give a more comprehensive picture of the effects of the approach. Practical implications Practical implications include the presentation of the ways different groups of users perceive the actual and future transformation of the street to make this place more user-friendly and sustainable, i.e. practical example of the co-design process. Originality/value This paper provide an overview of the possibilities of placemaking approach seen from the perspective of sustainable urban facilities management.

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The role of affect in feelings of obligation

Behavioral and Brain Sciences ◽

10.1017/s0140525x19002449 ◽

2020 ◽

Vol 43 ◽

Author(s):

Stefen Beeler-Duden ◽

Meltem Yucel ◽

Amrisha Vaish

Keyword(s):

Positive Emotions ◽

The Creation ◽

Sense Of Obligation

Abstract Tomasello offers a compelling account of the emergence of humans’ sense of obligation. We suggest that more needs to be said about the role of affect in the creation of obligations. We also argue that positive emotions such as gratitude evolved to encourage individuals to fulfill cooperative obligations without the negative quality that Tomasello proposes is inherent in obligations.

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Replication of Internal Biological Surfaces

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094802 ◽

1972 ◽

Vol 30 ◽

pp. 308-309

Author(s):

J. A. Nowell ◽

J. Pangborn ◽

W. S. Tyler

Keyword(s):

Da Vinci ◽

Leonardo Da Vinci ◽

Rat Lung ◽

Tubular Structures ◽

Cerebral Ventricles ◽

Internal Surfaces ◽

Biological Surfaces ◽

Dog Kidney ◽

Lung And Kidney ◽

Scanning Electron

Leonardo da Vinci in the 16th century, used injection replica techniques to study internal surfaces of the cerebral ventricles. Developments in replicating media have made it possible for modern morphologists to examine injection replicas of lung and kidney with the scanning electron microscope (SEM). Deeply concave surfaces and interrelationships to tubular structures are difficult to examine with the SEM. Injection replicas convert concavities to convexities and tubes to rods, overcoming these difficulties.Batson's plastic was injected into the renal artery of a horse kidney. Latex was injected into the pulmonary artery and cementex in the trachea of a cat. Following polymerization the tissues were removed by digestion in concentrated HCl. Slices of dog kidney were aldehyde fixed by immersion. Rat lung was aldehyde fixed by perfusion via the trachea at 30 cm H2O. Pieces of tissue 10 x 10 x 2 mm were critical point dried using CO2. Selected areas of replicas and tissues were coated with silver and gold and examined with the SEM.

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Fusion and Fission Processes in Exocytosis: Possible Roles for Synexin and Osmotic Lysis in the Two Events

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s1431927600002634 ◽

1980 ◽

Vol 38 ◽

pp. 594-597

Author(s):

H.B. Pollard ◽

C.E. Creutz ◽

C.J. Pazoles ◽

J.H. Scott

Keyword(s):

Small Molecules ◽

Chromaffin Cells ◽

Adrenal Glands ◽

General Concept ◽

Extracellular Calcium ◽

Large Protein ◽

Granule Membrane ◽

Osmotic Lysis ◽

Per Se ◽

Chemical Evidence

Exocytosis is a general concept describing secretion of enzymes, hormones and transmitters that are otherwise sequestered in intracellular granules. Chemical evidence for this concept was first gathered from studies on chromaffin cells in perfused adrenal glands, in which it was found that granule contents, including both large protein and small molecules such as adrenaline and ATP, were released together while the granule membrane was retained in the cell. A number of exhaustive reviews of this early work have been published and are summarized in Reference 1. The critical experiments demonstrating the importance of extracellular calcium for exocytosis per se were also first performed in this system (2,3), further indicating the substantial service given by chromaffin cells to those interested in secretory phenomena over the years.

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