scholarly journals Release Factor Inhibiting Antimicrobial Peptides Improve Nonstandard Amino Acid Incorporation in Wild-type Bacterial Cells

2020 ◽  
Vol 15 (7) ◽  
pp. 1852-1861 ◽  
Author(s):  
Erkin Kuru ◽  
Rosa-Maria Määttälä ◽  
Karen Noguera ◽  
Devon A. Stork ◽  
Kamesh Narasimhan ◽  
...  
Keyword(s):  
Amino Acid ◽  
Antimicrobial Peptides ◽  
Bacterial Cells ◽  
Release Factor ◽  
Wild Type ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals The effect of farnesol on amino acid incorporation by a wild-type and cell-wall variant strain ofCandida albicans

2005 ◽  
Vol 51 (8) ◽  
pp. 715-718 ◽  
Author(s):  
Phyllis C Braun
Keyword(s):  
Cell Wall ◽  
Candida Albicans ◽  
Amino Acid ◽  
Quorum Sensing ◽  
Cell Surface ◽  
Wild Type ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Ofcandida Albicans ◽  
Density Growth

Farnesol, a quorum sensing (QS) signal, is produced by Candida albicans during high density growth and has been found to inhibit morphogenesis. This QS auto-inducing signal was discovered to increase amino acid incorporation by C. albicans when concentrations of farnesol increased to 10 µg/mL in yeast nitrogen broth. Farnesol concentrations greater than 10 µg/mL abolished the enhanced incorporation, and the magnitude of the increased incorporation was dependent on cell-surface hydrophobicity.Key words: Candida albicans, farnesol, amino acid incorporation.

Performance of optimized noncanonical amino acid mutagenesis systems in the absence of release factor 1

2016 ◽  
Vol 12 (6) ◽  
pp. 1746-1749 ◽  
Author(s):  
Yunan Zheng ◽  
Marc J. Lajoie ◽  
James S. Italia ◽  
Melissa A. Chin ◽  
George M. Church ◽  
...  
Keyword(s):  
Amino Acid ◽  
Release Factor ◽  
Amino Acid Incorporation ◽  
E Coli ◽  
Acid Incorporation ◽  
Amino Acid Mutagenesis ◽  
Noncanonical Amino Acid

Unconditional deletion of RF1 in a genomically recoded E. coli enables multisite noncanonical amino acid incorporation by UAG suppression.

scholarly journals Transferability of N-terminal mutations of pyrrolysyl-tRNA synthetase in one species to that in another species on unnatural amino acid incorporation efficiency

Amino Acids ◽  
2020 ◽  
Author(s):  
Thomas L. Williams ◽  
Debra J. Iskandar ◽  
Alexander R. Nödling ◽  
Yurong Tan ◽  
Louis Y. P. Luk ◽  
...  
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Genetic Code ◽  
Unnatural Amino Acids ◽  
Trna Synthetase ◽  
Unnatural Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation ◽  
Genetic Code Expansion ◽  
Incorporation Efficiency

AbstractGenetic code expansion is a powerful technique for site-specific incorporation of an unnatural amino acid into a protein of interest. This technique relies on an orthogonal aminoacyl-tRNA synthetase/tRNA pair and has enabled incorporation of over 100 different unnatural amino acids into ribosomally synthesized proteins in cells. Pyrrolysyl-tRNA synthetase (PylRS) and its cognate tRNA from Methanosarcina species are arguably the most widely used orthogonal pair. Here, we investigated whether beneficial effect in unnatural amino acid incorporation caused by N-terminal mutations in PylRS of one species is transferable to PylRS of another species. It was shown that conserved mutations on the N-terminal domain of MmPylRS improved the unnatural amino acid incorporation efficiency up to five folds. As MbPylRS shares high sequence identity to MmPylRS, and the two homologs are often used interchangeably, we examined incorporation of five unnatural amino acids by four MbPylRS variants at two temperatures. Our results indicate that the beneficial N-terminal mutations in MmPylRS did not improve unnatural amino acid incorporation efficiency by MbPylRS. Knowledge from this work contributes to our understanding of PylRS homologs which are needed to improve the technique of genetic code expansion in the future.

scholarly journals FRUCTOSE-AMINO ACIDS IN LIVER: STIMULI OF AMINO ACID INCORPORATION IN VITRO

1955 ◽  
Vol 215 (1) ◽  
pp. 111-124 ◽  
Author(s):  
Henry Borsook ◽  
Adolph Abrams ◽  
Peter H. Lowy
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation

scholarly journals INDEPENDENT ANTAGONISM OF AMINO ACID INCORPORATION INTO PROTEIN

1954 ◽  
Vol 210 (2) ◽  
pp. 837-849 ◽  
Author(s):  
M. Rabinovitz ◽  
Margaret E. Olson ◽  
David M. Greenberg
Keyword(s):  
Amino Acid ◽  
Amino Acid Incorporation ◽  
Acid Incorporation
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